【摘要】：目的:基于PCR array技術(shù)觀察脾虛痰濁巴馬小型豬回腸線粒體能量代謝相關(guān)基因差異性表達(dá)及健脾祛痰化瘀方藥的干預(yù)作用。方法:隨機(jī)將9只健康的廣西巴馬小型豬分為正常組、模型組和中藥治療組,每組3只,正常組給予普通飼料飼喂,模型組和中藥治療組采用疲勞過度聯(lián)合高脂飲食飼喂的方法進(jìn)行脾虛痰濁動(dòng)脈粥樣硬化模型復(fù)制,造模24周后,中藥治療組給予健脾祛痰化瘀方藥,48周后,觀察巴馬小型豬行為學(xué)變化,呼吸機(jī)麻醉后取材,分離冠狀動(dòng)脈,應(yīng)用PCR array技術(shù)觀察巴馬小型豬回腸線粒體能量代謝相關(guān)基因變化。結(jié)果:造模24周后,巴馬小型豬出現(xiàn)食少且不欲飲食、神疲乏力、倦怠等明顯脾虛證候;PCR array結(jié)果顯示:與空白對照組比較,模型組變化水平在2倍以上,且經(jīng)中藥干預(yù)后表達(dá)水平異常被明顯糾正的基因共10個(gè),分別為:ATP4B、NDUFS2、NDUFS3、NDUFS4、PPA2、SDHB、SDHC、COX17、NDUFA9、NDUFB1。結(jié)論:健脾祛痰化瘀方藥對脾虛痰濁巴馬小型豬的干預(yù)作用可能通過對回腸ATP4B、NDUFS2、NDUFS3等與線粒體能量代謝相關(guān)基因?qū)崿F(xiàn)。
[Abstract]:Objective: to observe the differentially expressed genes related to energy metabolism in ileum mitochondria of spleen deficiency phlegm turbid Bama miniature pig and the intervention effect of invigorating spleen eliminating phlegm and removing blood stasis by PCR array technique. Methods: nine healthy Guangxi Bama miniature pigs were randomly divided into normal group, model group and traditional Chinese medicine treatment group with 3 pigs in each group. The model group and traditional Chinese medicine treatment group were fed with excessive fatigue combined with high fat diet to replicate the model of spleen deficiency and phlegm turbid artery atherosclerosis. After 24 weeks of making the model, the traditional Chinese medicine treatment group was given the prescription of invigorating spleen, removing phlegm and removing blood stasis for 48 weeks. The behavioral changes of Bama miniature pigs were observed, and the coronary arteries were isolated after ventilator anesthesia. The changes of genes related to mitochondrial energy metabolism in ileum of Bama miniature pigs were observed by PCR array technique. Results: after 24 weeks of model making, Bama mini-pigs showed obvious spleen deficiency syndrome, such as eating less and not wanting to eat, fatigue and burnout. The results showed that compared with the blank control group, the change level of the model group was more than 2 times. After intervention with Chinese medicine, there were 10 genes whose abnormal expression level was obviously corrected, namely: 1 ATP-4B, NDUFS2, NDUFS3, NDUFS4, NDUFS4PPA2, SDHBSDHCN, COX17NDUFA9, NDUFB1, and NDUFB1, NDUFA9 and NDUFB1 respectively. Conclusion: the intervention effect of invigorating spleen and removing phlegm and removing blood stasis on spleen deficiency and phlegm turbid miniature pig may be realized by ATP4B,NDUFS2,NDUFS3 and other genes related to mitochondrial energy metabolism.
【作者單位】： 遼寧中醫(yī)藥大學(xué)中醫(yī)臟象理論及應(yīng)用教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃(973計(jì)劃)項(xiàng)目(2013CB531704)
【分類號(hào)】：R285.5
，

本文編號(hào)：2256002