【摘要】：目的在了解相關(guān)動(dòng)物造模實(shí)驗(yàn)資料的前提下，通過(guò)白酒灌胃，并輔助長(zhǎng)期激怒的方法使大鼠酒精性肝炎肝腎陰虛證的動(dòng)物模型復(fù)制成功，檢測(cè)給不同劑量的一貫煎加味后各組大鼠的血清生化指標(biāo)，并觀察各組大鼠肝臟病理切片的變化，探討一貫煎加味對(duì)大鼠酒精性肝炎肝腎陰虛證的防治作用。 方法1.一貫煎加味預(yù)防大鼠酒精性肝炎肝腎陰虛證：40只200±10g雄性SD大鼠，飼養(yǎng)14天使其充分適應(yīng)環(huán)境，隨機(jī)分為5組，每組8只。包括對(duì)照組，模型組，一貫煎加味預(yù)防低劑量組，一貫煎加味預(yù)防中劑量組，一貫煎加味高劑量組。除對(duì)照組外，其余各組大鼠均雙后肢束縛，引起大鼠激怒，待激怒1小時(shí)后，，對(duì)照組按其習(xí)性給予相應(yīng)飼料，同時(shí)用符合比例的蒸餾水灌胃；其他組別給予飼料的同時(shí)，每天根據(jù)大鼠重量相應(yīng)給予9g/kg劑量的40。白酒進(jìn)行灌胃，直到42天，除對(duì)照組和模型組外，其它各組大鼠在酒精灌胃1h后，使用符合比例的濃縮中藥灌入大鼠體內(nèi)。模型組在酒精灌胃1h后使用符合比例的蒸餾水進(jìn)行灌胃，堅(jiān)持灌胃42天。最后一次灌胃后，將飼料和水從鼠籠撤掉約半天的時(shí)間，按比例將水合氯醛配置為6%，按照體重計(jì)算藥量，麻醉大鼠，從腹主動(dòng)脈取血，并把血分成兩部分，一部分血用于測(cè)定雌二醇（E2）、睪酮（T）以及肝功各項(xiàng)指標(biāo)；另一部分血用于測(cè)定環(huán)磷酸腺苷(cAMP)，并取肝臟在生理鹽水中反復(fù)沖洗，直至沒(méi)有淤血，將每組大鼠各自的肝臟取其中的一部分組織泡入福爾馬林溶液中固定做病理切片。 2.一貫煎加味治療大鼠酒精性肝炎肝腎陰虛證：將24只250±10g造模成功的雄性SD大鼠隨機(jī)分為3組，每組8只。包括一貫煎加味治療低劑量組、一貫煎加味治療中劑量組、一貫煎加味治療高劑量組。對(duì)照組和模型組按其習(xí)性給予相應(yīng)飼料和水，同時(shí)用符合比例的蒸餾水灌胃；其它組在給予飼料的同時(shí)，使用符合比例的濃縮中藥灌入大鼠體內(nèi)。堅(jiān)持灌胃42天。最后一次灌胃后，將飼料和水從鼠籠撤掉約半天的時(shí)間，按比例將水合氯醛配置為6%，腹主動(dòng)脈取血，將血分成兩部分，一部分血用于測(cè)定雌二醇（E2）、睪酮（T）以及肝功各項(xiàng)指標(biāo)；另一部分血用于測(cè)定環(huán)磷酸腺苷(cAMP)。并取肝臟在生理鹽水中反復(fù)沖洗，直至沒(méi)有淤血，將每組大鼠各自的肝臟取其中的一部分組織泡入福爾馬林溶液中固定做病理切片。 結(jié)果一貫煎加味能夠?qū)τ捎?0。白酒灌胃配合激怒法引起的大鼠飲食下降、精神欠佳等方面有一定的改善作用；對(duì)40。白酒灌胃引起的血清谷丙轉(zhuǎn)氨酶（ALT）、谷草轉(zhuǎn)氨酶（AST）、AST/ALT、總膽紅素(TBIL)、γ-谷氨酰轉(zhuǎn)移酶(γ-GT)、堿性磷酸酶（AKP）的升高有一定的防治作用；對(duì)激怒法引起的雌二醇（E2）、E2/T、環(huán)磷酸腺苷(cAMP)的升高有一定的防治作用；對(duì)40。白酒灌胃引起的總蛋白（TP）、白蛋白(Alb)的降低有防治作用；對(duì)激怒法引起的睪酮（T）的降低有防治作用；對(duì)大鼠因酒精灌胃導(dǎo)致肝細(xì)胞損傷有一定的防治作用。并且，一貫煎加味預(yù)防大鼠酒精性肝炎肝腎陰虛方面，中劑量和高劑量的預(yù)防效果較好；一貫煎加味治療大鼠酒精性肝炎肝腎陰虛方面，高劑量的治療效果較好。 結(jié)論大鼠酒精性肝炎肝腎陰虛證造模成功；一貫煎加味對(duì)酒精性肝炎肝腎陰虛證有一定的預(yù)防作用；一貫煎加味對(duì)大鼠酒精性肝炎肝腎陰虛證也有不錯(cuò)的治療效果。
[Abstract]:Objective To make the animal model of alcoholic hepatitis with Yin deficiency of liver and kidney succeed by intragastric administration of liquor and long-term irritation, and to detect the serum biochemical indexes of rats in each group after different doses of consistently decocting and flavoring, and to observe the changes of liver pathological sections of rats in each group. Objective: To investigate the preventive and therapeutic effects of Liwei Decoction on alcoholic liver hepatitis and yin deficiency syndrome in rats.
Methods 1. Gongguan Jiawei Decoction for Preventing Liver and Kidney Yin Deficiency Syndrome in Rats with Alcoholic Hepatitis: 40 male SD rats were fed for 14 days. They were randomly divided into 5 groups, 8 rats in each group. The rats in the other groups were restrained by hind limbs, which caused the rats to be angry. After one hour of anger, the control group was given the corresponding diet according to their habits and at the same time, the rats in the other groups were given the corresponding dosage of 40. The rats in the model group were fed with distilled water for 42 days after one hour of alcohol administration. After the last gastric administration, the feed and water were removed from the cage for about half a day. The chloral hydrate was proportionally allocated to 6%, and the rats in the model group were fed with distilled water for 42 days. The rats were anesthetized by calculating the dosage according to their body weight. The blood was taken from the abdominal aorta and divided into two parts. One part of the blood was used to measure estradiol (E2), testosterone (T) and liver function. The other part of the blood was used to measure cyclic adenosine monophosphate (cAMP) and the liver was washed repeatedly in normal saline until no congestion occurred. Some of the tissues were injected into formalin solution for pathological sections.
2. Treating alcoholic hepatitis of rats with liver and kidney yin deficiency syndrome by "Gongguan Jiawei": 24 male SD rats were randomly divided into 3 groups, 8 rats in each group, including low dose group treated by "Gongguan Jiawei", "Gongguan Jiawei", "Gongguan Jiawei", "Gongguan Jiawei" group treated by "Gongguan Jiawei", "Gongguan Jiawei" group treated by "Gongguan Jiawei". The control group and model group were The rats in the other groups were fed with the same proportion of concentrated Chinese herbs for 42 days. After the last feeding, the feed and water were removed from the cage for about half a day. Chloral hydrate was proportionally allocated to 6%. Blood was taken from the abdominal aorta and divided into two parts. One part of the blood was used to measure estradiol (E2), testosterone (T) and liver function. The other part of the blood was used to measure cyclic adenosine monophosphate (cAMP).
Results Yiguan Jiawei Decoction could improve the diet and mental health of rats caused by 40.Liquor-infusion combined with irritation; 40.Liquor-infused could increase serum alanine aminotransferase (ALT), glutamic oxaloacetic aminotransferase (AST), AST/ALT, total bilirubin (TBIL), gamma-glutamyltransferase (gamma-GT) and alkaline phosphatase (AKP). It has some preventive and therapeutic effects on the elevation of estradiol (E2), E2/T and cAMP induced by irritation, the decrease of total protein (TP) and albumin (Alb) induced by liquor gavage, the decrease of testosterone (T) induced by irritation, and the liver induced by alcohol gavage in rats. In addition, the medium dose and high dose of Gongguan decoction have better preventive effect on liver and kidney yin deficiency of alcoholic hepatitis in rats, and the high dose of Gongguan decoction has better therapeutic effect on liver and kidney yin deficiency of alcoholic hepatitis in rats.
Conclusion The model of liver-kidney Yin deficiency syndrome in rats with alcoholic hepatitis is successful, the modified decoction has a certain preventive effect on liver-kidney Yin deficiency syndrome in alcoholic hepatitis, and the modified decoction has a good therapeutic effect on liver-kidney Yin deficiency syndrome in rats with alcoholic hepatitis.
【學(xué)位授予單位】：山西中醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R285.5

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