本文選題：蒙醫(yī) + 灸療法　； 參考：《北京中醫(yī)藥大學(xué)》2014年博士論文

【摘要】：研究目的 1.本課題根據(jù)蒙醫(yī)文獻記載及前期研究基礎(chǔ)上提出蒙醫(yī)灸療法可能對老年性癡呆模型AD大鼠有保護和改善腦神經(jīng)細(xì)胞損傷,改善腦神經(jīng)功能回復(fù)的假設(shè),采用蒙醫(yī)灸療法治療AD大鼠的實驗研究方法,闡明蒙醫(yī)灸療法對老年性癡呆病的治療作用及其機理。 2.采用大鼠皮下注射D-半乳糖和雙側(cè)海馬注射Aβ25-35建立大鼠老年性癡呆病理模型,以蒙醫(yī)“頂會穴”、“心穴”和“胃穴”為切入點,設(shè)置正常組、假手術(shù)組、模型組、灸療組和西藥組,觀察灸療法對AD大鼠的學(xué)習(xí)記憶能力、相關(guān)膽堿、氧化、自由基酶的含量,腦組織神經(jīng)細(xì)胞病理改變、腦組織相關(guān)基因的mRNA和蛋白表達的影響,闡明蒙醫(yī)灸療法治療AD的作用及其機理。 研究方法 1.動物選擇及篩選方法：選用體質(zhì)量220±20(g)、健康清潔級雄性SD大鼠,將大鼠分別進行MorriS水迷宮測試,剔除天生癡呆大鼠,選出合格大鼠70只。 2.大鼠分組及造模：將70只大鼠按體質(zhì)量隨機分為5組,正常組、假手術(shù)組、模型組、灸療組和西藥組,每組14只。前6周：模型組、灸療組和西藥組三組大鼠均頸背部皮下注射D-半乳糖(150mg/kg.d);假手術(shù)組頸部皮下注射生理鹽水(150mg/kg.d),每天一次,連續(xù)6周；正常組不予處理。第7周開始：除正常組外其余四組大鼠立體定位海馬,進行顱腦手術(shù),模型組、灸療組和西藥組三組大鼠將準(zhǔn)備好的聚集態(tài)Aβ25-35(濃度10ug/ul)緩慢注入雙側(cè)海馬,每側(cè)luL在5min內(nèi)注入。假手術(shù)組雙側(cè)海馬注入等劑量的無菌生理鹽水；正常組不予處理。 3.模型成功檢測方法：造模完成2天后進行水迷宮實驗,得出造模后大鼠的平均逃避潛伏期。凡每只大鼠的平均逃避潛伏期較造模前下降1/3者為造模成功。只有模型成功者才進入下一步實驗,若出現(xiàn)死亡或不符合條件的大鼠則予以剔除,并遵循隨機原則補齊動物。 4.治療方法：造模成功后,開始治療。灸療組大鼠取其“頂會穴”、“心穴”和“胃穴”,每日一穴,每次治療15mmin,自貼式艾柱施灸,三穴交替,10天為-療程,一療程后休一天,再治療,共治療2個療程：西藥治療組給予臨床治療老年性癡呆的藥物吡拉西坦片(腦復(fù)康)0.92mg/kg灌胃,共治療20天；模型組和假手術(shù)組大鼠與灸療組相同的抓取和固定,并與西藥組相同劑量的生理鹽水灌胃；正常組不給予任何處理。 5.取材與指標(biāo)檢測：治療結(jié)束后,全部大鼠進行迷宮實驗、取材和指標(biāo)檢測。(1)每組14只大鼠均采用Morris水迷宮實驗進行行為學(xué)測試,計算出每只大鼠平均逃避潛伏期、經(jīng)過平臺次數(shù)及經(jīng)過有效區(qū)域的比例；(2)每組4只大鼠進行心臟灌注固定后進行Nissl和TUNEL染色檢測海馬神經(jīng)元的變化,損傷情況及凋亡情況；(3)每組10只大鼠麻醉,置于冰盒上分離皮質(zhì)和左側(cè)海馬,稱重,凍存,采用紫外分光光度計,測定皮質(zhì)和海馬組織的MDA含量和SOD、ChAT、 AchE的活性含量。(4)每組6只大鼠麻醉,置于冰盒上分離右側(cè)海馬,稱重,凍存,采用實時定量RT-PCR方法檢測海馬組織Bcl-2、Bax的mRNA轉(zhuǎn)錄水平；采用Western Blot法測定海馬組織Bcl-2、Bax的蛋白表達。 6.統(tǒng)計學(xué)處理：采用SPSS19.0軟件進行統(tǒng)計學(xué)處理,數(shù)據(jù)以均數(shù)±標(biāo)準(zhǔn)差(x±s)表示,多組間比較用F檢驗,組間兩兩比較用LSD檢驗,P0.05為顯著性差異。 結(jié)果 1.蒙醫(yī)灸療法對AD大鼠學(xué)習(xí)記憶行為學(xué)的影響 (1)治療結(jié)束后,各組大鼠定位航行試驗,“1-5天平均潛伏期”和“經(jīng)過有效區(qū)域比例”比較,模型組與正常組比較“1-5天平均潛伏期”顯著延長(P0.05)、“經(jīng)過有效區(qū)域比例”顯著減少(P0.05),模型組與假手術(shù)組比較“1-5天平均潛伏期”顯著延長(P0.01)、“經(jīng)過有效區(qū)域比例”顯著減少(P0.01),表明AD模型制作成功；灸療組、西藥組與模型組比較,“1-5天平均潛伏期”均顯著縮短(P0.01)、灸療組與模型組比較,“經(jīng)過有效區(qū)域比例”顯著提高(P0.01),西藥組與模型組比較,“經(jīng)過有效區(qū)域比例”無顯著差異(P0.05),表明蒙醫(yī)灸療和西藥對AD學(xué)習(xí)記憶功能有提高作用。 (2)治療結(jié)束后,各組大鼠空間搜索試驗,“經(jīng)過平臺所在區(qū)的次數(shù)”、“經(jīng)過有效區(qū)域比例”比較,模型組與正常組比較“經(jīng)過平臺所在區(qū)的次數(shù)”和“經(jīng)過有效區(qū)域比例”均顯著減少(P0.05)、模型組與假手術(shù)組比較“經(jīng)過平臺所在區(qū)的次數(shù)”和“經(jīng)過有效區(qū)域比例”均顯著減少(P0.01),表明AD模型制作成功；灸療組與模型組比較,“經(jīng)過平臺所在區(qū)的次數(shù)”和“經(jīng)過有效區(qū)域比例”均顯著提高(P0.01),西藥組與模型組比較,“經(jīng)過平臺所在區(qū)的次數(shù)”顯著提高(P0.01),“經(jīng)過有效區(qū)域比例”無顯著差異(P0.05),灸療組與西藥組比較“經(jīng)過有效區(qū)域比例”有顯著增多(P0.05)。提示蒙醫(yī)灸療對AD記憶功能有顯著作用,且比西藥(吡拉西坦片)有略優(yōu)于的趨勢。 2.蒙醫(yī)灸療法對AD大鼠海馬神經(jīng)細(xì)胞的影響 (1) Nissl染色結(jié)果顯示：與正常組和假手術(shù)組相比,模型組大鼠大腦海馬區(qū)Nissl小體的數(shù)量明顯減少(P0.01),染色較淺,排列不齊,表明AD模型制作成功；經(jīng)吡拉西坦片治療后,Nissl染色形態(tài)學(xué)觀察指標(biāo)較模型組明顯改善(P0.05)；蒙醫(yī)灸療法治療后,大鼠海馬區(qū)Nissl染色較深,Nissl小體的數(shù)量明顯增加(P0.01),排列也較有序；蒙醫(yī)灸療法與西藥組比較,Nissl染色較深,Nissl小體的數(shù)量明顯比西藥組多(P0.05)。表明,蒙醫(yī)灸療法和西藥治療能減輕AD神經(jīng)元的損傷,并蒙醫(yī)灸療法作用優(yōu)于吡拉西坦。 (2) TUNEL染色結(jié)果顯示：正常組和假手術(shù)組大鼠海馬區(qū)可偶見TUNEL陽性細(xì)胞,而模型組大鼠海馬區(qū)存在較多的TUNEL陽性細(xì)胞(P0.01),表明,AD模型制作成功；經(jīng)吡拉西坦片治療后,大鼠海馬區(qū)陽性細(xì)胞較模型組有所減少(P0.01),蒙醫(yī)灸療組偶見陽性細(xì)胞,與模型組對照有明顯減少(P0.01),蒙醫(yī)灸療組與西藥組比較,TUNEL陽性細(xì)胞比西藥組明顯少(P0.05)。表明,蒙醫(yī)灸療法和西藥治療均能逆轉(zhuǎn)AD神經(jīng)細(xì)胞的凋亡,并蒙醫(yī)灸療法的作用優(yōu)于吡拉西坦片。 3.蒙醫(yī)灸療對AD大鼠的腦組織MDA、SOD和ChAT、AchE的影響 (1)各組大鼠大腦皮質(zhì)和海馬組織內(nèi)SOD、MDA的測定 ①各組大鼠皮質(zhì)和海馬組織的SOD活性比較,與正常組和假手術(shù)組比較,模型組皮質(zhì)和海馬SOD活性顯著降低(P0.01),灸療組、西藥組與模型組比較,皮質(zhì)和海馬SOD活性均有顯著提高(P0.01),西藥組與灸療組之間比較無顯著差異(P0.05)；假手術(shù)組與正常組比較,皮質(zhì)和海馬SOD活性有顯著提高(P0.01,P0.05)。 ②各組大鼠皮質(zhì)和海馬組織的MDA含量比較,與正常組和假手術(shù)組比較,模型組皮質(zhì)和海馬MDA含量均顯著提高(P0.01),灸療組、西藥組與模型組比較,皮質(zhì)和海馬MDA均有顯著降低(P0.01),西藥組與灸療組之間比較無顯著差異(P0.05)；假手術(shù)組與正常組比較,皮質(zhì)MDA含量有顯著減少(P0.01),海馬MDA含量無顯著差異(P0.05)。(2)各組大鼠大腦皮質(zhì)和海馬組織內(nèi)ChAT、AchE的測定 ①各組大鼠大腦皮質(zhì)和海馬組織的ChAT活性比較,與正常組和假手術(shù)組比較,模型組皮質(zhì)和海馬ChAT活性均顯著降低(P0.01),灸療組、西藥組與模型組比較,皮質(zhì)和海馬ChAT活性均有顯著提高(P0.01),西藥組與灸療組之間比較無顯著差異(P0.05)；假手術(shù)組與正常組比較無顯著差異(P0.05)：灸療組與正常組比較,皮質(zhì)ChAT活性有顯著差異(P0.05)。西藥組與正常組和假手術(shù)組比較,皮質(zhì)和海馬ChAT活性均有顯著降低(P0.01)。 ②各組大鼠大腦皮質(zhì)和海馬組織的AchE舌性比較,與正常組和假手術(shù)組比較,模型組皮質(zhì)和海馬AchE活性均顯著降低(P0.01),灸療組、西藥組與模型組比較,皮質(zhì)和海馬AchE活性均有顯著提高(P0.01),西藥組與灸療組之間比較無顯著差異(P0.05)；假手術(shù)組與正常組比較無顯著差異(P0.05)。 4.蒙醫(yī)灸療法對AD大鼠海馬組織Bcl-2、Bax的：mRNA和蛋白表達的影響 (1)各組大鼠海馬組織Bcl-2的mRNA和蛋白表達比較,與正常組和假手術(shù)組比較,模型組Bcl-2的mRNA和蛋白表達水平顯著降低(P0.01),灸療組、西藥組與模型組比較,Bcl-2的mRNA和蛋白表達水平有顯著提高(P0.01、P0.05),西藥組與灸療組之間比較無顯著差異(P0.05)；假手術(shù)組與正常組比較,無顯著差異(P0.05)。 (2)各組大鼠海馬組織Bax的mRNA和蛋白表達比較,與正常組和假手術(shù)組比較,模型組Bax的mRNA和蛋白表達水平顯著提高(P0.01),灸療組和西藥組與模型組比較,Bax的mRNA和蛋白表達水平均有顯著降低(P0.01),西藥組與灸療組之間比較無顯著差異(P0.05)；假手術(shù)組與正常組比較,無顯著差異(P0.05)。 結(jié)論 1.本研究采用大鼠皮下注射D-半乳糖和雙側(cè)海馬注射Aβ25-35建立大鼠老年性癡呆模型,能較好的模擬AD學(xué)習(xí)記憶障礙、神經(jīng)元損傷、神經(jīng)細(xì)胞凋亡等行為學(xué)和病理學(xué)方面的特征,更接近AD的病理表現(xiàn),具有較好的定位性和實用性。 2.蒙醫(yī)灸療法能改善AD大鼠的行為學(xué)功能,提高學(xué)習(xí)和記憶能力,對AD有防治作用。 3.蒙醫(yī)灸療法能降低AD大鼠大腦海馬組織神經(jīng)細(xì)胞的損傷,抑制神經(jīng)細(xì)胞的凋亡,對AD有防治作用。 4.蒙醫(yī)灸療法能提高AD大鼠腦組織的SOD活性,降低MDA含量,減輕脂質(zhì)過氧化反應(yīng),提高抗氧化酶活性,清除自由基,抗衰老,從而防治AD病的發(fā)生和發(fā)展。 5.蒙醫(yī)灸療法能提高AD大鼠腦組織的ChAT、AchE的活性,保護腦組織酶活性,改善腦組織能量代謝障礙,從而可能改善AD的膽堿能系統(tǒng)功能障礙。 6.蒙醫(yī)灸療法對AD大鼠海馬組織的Bcl-2的mRNA和蛋白有上調(diào)作用,對Bax的mRNA和蛋白有下調(diào)作用,對AD的腦神經(jīng)細(xì)胞凋亡有很好的抑制作用。 7.對AD大鼠的蒙醫(yī)“頂會穴”、“心穴”和“胃穴”實施蒙醫(yī)灸療法后,有改善學(xué)習(xí)記憶功能,減少大腦海馬神經(jīng)細(xì)胞的損傷,抑制神經(jīng)細(xì)胞凋亡等作用,其作用機理與提高大腦組織SOD活性、減少MDA含量,提高ChAT、AchE的活性和上調(diào)海馬組織中Bcl-2的mRNA和蛋白表達,下調(diào)Bax的mRNA和蛋白表達等有密切關(guān)系。 8.蒙醫(yī)灸療法與西藥(吡拉西坦片)對AD大鼠的治療作用比較,蒙醫(yī)灸療在改善學(xué)習(xí)記憶及海馬神經(jīng)細(xì)胞抗凋亡方面有較明顯的作用,證明蒙醫(yī)灸療法對AD的作用較優(yōu)于吡拉西坦片的趨勢。
[Abstract]:research objective
1. based on the literature of Mongolian medical literature and the previous study, the Mongolian medicine moxibustion therapy may protect and improve the brain nerve cell damage and improve the brain function recovery hypothesis, and the Mongolian medicine moxibustion therapy is used to treat AD rats, and the Mongolian medicine moxibustion therapy for Alzheimer's disease is clarified on the basis of the Mongolian medicine AD rats. The therapeutic effect and its mechanism.
2. the rats were injected subcutaneous injection of D- galactose and bilateral hippocampal injection of A beta 25-35 to establish the pathological model of Alzheimer's disease in rats. The normal group, sham operation group, model group, moxibustion group and Western medicine group were set up in the Mongolian medicine "top cavern", "heart point" and "gastric acupoint". The learning and memory ability of moxibustion therapy on AD rats was observed, choline and oxidation were observed. The effects of the content of free radical enzymes, the pathological changes of brain tissue, the mRNA and protein expression of brain tissue related genes, and the effect of Mongolian medicine moxibustion therapy on the treatment of AD and its mechanism.
research method
1. animal selection and screening methods: the body mass of 220 + 20 (g), healthy and clean male SD rats, the rats were tested by MorriS water maze, and the rats were eliminated, and 70 qualified rats were selected.
2. rats were divided into groups and models: 70 rats were randomly divided into 5 groups according to body mass, normal group, sham operation group, model group, moxibustion group and Western medicine group, 14 rats in each group. The model group, moxibustion group and Western medicine group were injected with D- galactose (150mg/kg.d) subcutaneously in the neck and back of the moxibustion group and the western medicine group, and the sham operation group was subcutaneously injected with saline (150mg/kg.d), daily neck saline (150mg/kg.d), daily neck subcutaneous injection (150mg/kg.d), every day. At one time for 6 weeks, the normal group did not handle the treatment. Seventh weeks began: the other four groups of rats, except the normal group, stereotaxic hippocampus, performed craniocerebral operation, model group, moxibustion group and Western medicine group three rats, the prepared A beta 25-35 (concentration 10ug/ul) was slowly injected into the bilateral hippocampus, each side luL was injected into the 5min. The bilateral hippocampus injection in the sham operation group was injected into the hippocampus. The rats in the normal group were not treated with equal dose of sterile saline.
3. model successful detection method: the water maze experiment was carried out after the model was completed after 2 days. The average escape latency of rats after the model was obtained. The average escape latency of each rat was more successful than that of 1/3 before the model. Only the model successes entered the next experiment, and the rats who died or did not conform to the conditions were eliminated, And follow random principles to complement animals.
4. treatment methods: after the success of the model, start the treatment. The moxibustion group took the "cacupoint point", "heart point" and "stomach point", one acupoint daily, each treatment 15mmin, the Three Acupoints alternately, 10 days as a course of treatment, one day after a course of treatment, and then treated for 2 courses of treatment: the western medicine treatment group was given clinical treatment of Alzheimer's disease. The drug Piracetam Tablets (brain Fukang) 0.92mg/kg was administered to the stomach for 20 days. The rats in the model group and the sham operation group were captured and fixed with the moxibustion group, and the same dose of saline was given to the western medicine group. The normal group did not give any treatment.
5. material and index test: after the treatment, all rats were tested by labyrinth experiment, material and index test. (1) 14 rats in each group were tested by Morris water maze test. The average escape latency of each rat was calculated, and the number of platform times and the proportion of effective areas were passed. (2) 4 rats in each group were perfused with heart perfusion. After fixation, Nissl and TUNEL staining were performed to detect the changes in hippocampal neurons, damage and apoptosis. (3) 10 rats in each group were anesthetized, placed on the ice box and left hippocampus, weighing, frozen, and using ultraviolet spectrophotometer to determine the MDA content of cortex and hippocampus and the activity of SOD, ChAT, AchE. (4) 6 rats in each group After anesthesia, the right hippocampus was separated on the ice box, weighed and frozen. The mRNA transcriptional level of hippocampal Bcl-2, Bax was detected by real-time quantitative RT-PCR, and the protein expression of Bcl-2 and Bax in hippocampus was measured by Western Blot.
6. statistical processing: SPSS19.0 software was used for statistical processing. The data were expressed with mean number + standard deviation (x + s). F test was used in multiple groups, and 22 of groups were compared with LSD test, and P0.05 was significant difference.
Result
1. the effect of Mongolian medicine moxibustion on learning and memory behavior of AD rats
(1) after the treatment, the navigation test of the rats in each group, the average incubation period of 1-5 days and the ratio of the effective area were compared. The model group compared with the normal group. The average incubation period of the 1-5 days was significantly prolonged (P0.05), and the "effective area ratio" was significantly reduced (P0.05). The model group was compared with the sham operation group for the average incubation period of 1-5 days. Significantly prolonged (P0.01), "through effective regional proportion" significantly reduced (P0.01), indicating the success of the AD model, moxibustion group, western medicine group and model group, "1-5 days average latency" significantly shortened (P0.01), moxibustion group and model group, "the effective area ratio" significantly improved (P0.01), western medicine group and model group, "the" There was no significant difference in the proportion of effective areas (P0.05), indicating that Mongolian medicine moxibustion and Western medicine could improve the learning and memory function of AD.
(2) after the treatment, the space search test of the rats in each group, "the number of the area in the platform", "through the effective area ratio", the model group and the normal group compared "the number of the platform area" and "the effective area ratio" significantly decreased (P0.05), the model group and the sham operation group "through the platform area" The number of times "and" the ratio of effective area "decreased significantly (P0.01), indicating that the AD model was successfully made. Compared with the model group, the number of the moxibustion group and the model group increased significantly (P0.01), and the number of the western medicine group and the model group was significantly higher (P0.01). There was no significant difference between the "effective area ratio" (P0.05). The moxibustion group and the western medicine group had a significant increase in the "effective area ratio" (P0.05). It suggested that moxibustion in Mongolian medicine has a significant effect on the memory function of AD, and is slightly better than the western medicine (Piracetam Tablets).
2. the effect of Mongolian medicine moxibustion on hippocampal neurons in AD rats
(1) the results of Nissl staining showed that compared with the normal group and the sham operation group, the number of Nissl corpuscles in the hippocampus of the model group was significantly reduced (P0.01), the staining was shallow, and the arrangement of the AD model was successful. After the Piracetam Tablets treatment, the morphological observation index of Nissl staining was significantly improved (P0.05), and the Mongolian medicine moxibustion therapy was better than that of the model group. After treatment, the Nissl staining in the hippocampus of the rats was deeper, the number of Nissl corpuscles increased obviously (P0.01) and the arrangement was more orderly. Compared with the western medicine group, the Mongolian medicine moxibustion therapy was much deeper than the western medicine group. The number of Nissl corpuscles was much more than that of the western medicine group (P0.05). It showed that the Mongolian medicine moxibustion and Western medicine treatment could reduce the injury of AD neurons, and the Mongolian medicine moxibustion therapy was superior to the moxibustion therapy of Mongolian medicine. In piracetam.
(2) the results of TUNEL staining showed that TUNEL positive cells could be seen in the hippocampus of the normal group and the sham operation group, while there were more TUNEL positive cells (P0.01) in the hippocampus of the model group, indicating that the AD model was successfully made. After the treatment of Piracetam Tablets, the positive cells in the hippocampus of the rats decreased (P0.01) and the moxibustion group in the Mongolian medicine group was even less than that of the model group (P0.01). Compared with the model group, the positive cells were significantly reduced (P0.01). Compared with the western medicine group, the moxibustion group in Mongolian medicine and the western medicine group were significantly less than the western medicine group (P0.05). It showed that the Mongolian medicine moxibustion therapy and the western medicine treatment could reverse the apoptosis of the AD nerve cells, and the effect of Mongolian medicine moxibustion was better than that of the Piracetam Tablets.
3. effect of Mongolian medicine moxibustion on MDA, SOD and ChAT, AchE in AD rats
(1) determination of SOD and MDA in cerebral cortex and hippocampus of rats in each group.
Compared with the normal group and the sham operation group, the activity of cortical and hippocampal SOD in the model group was significantly lower than that of the normal group and the sham group (P0.01). Compared with the model group, the activity of the cortex and hippocampus of the model group was significantly improved (P0.01), and there was no significant difference between the western medicine group and the moxibustion group (P0.05), and the sham operation between the western medicine group and the moxibustion group was not significant (P0.05), and the sham operation of the group was compared with the normal group and the sham operation group (P0.01). Compared with normal group, the activity of SOD in cortex and hippocampus increased significantly (P0.01, P0.05).
Compared with the normal group and the sham operation group, the content of the cortex and hippocampal MDA in the model group was significantly increased (P0.01). Compared with the model group, the cortex and hippocampus MDA were significantly decreased (P0.01), and there was no significant difference between the western medicine group and the moxibustion group (P0.05), and the sham operation group had no significant difference (P0.01). Compared with the normal group, the content of cortical MDA was significantly decreased (P0.01), and there was no significant difference in the content of MDA in the hippocampus (P0.05). (2) the determination of ChAT and AchE in the cerebral cortex and hippocampus of rats.
Compared with the normal group and the sham operation group, the activity of ChAT in the cortex and hippocampus of the model group was significantly lower than that of the normal group and the sham group (P0.01). Compared with the model group, the activity of the cortex and hippocampus of the model group was significantly increased (P0.01), and there was no significant difference between the western medicine group and the moxibustion group (P0.05), compared with the normal group and the sham operation group (P0.01). There was no significant difference between the sham operation group and the normal group (P0.05): compared with the normal group, the activity of cortex ChAT in the moxibustion group was significantly different (P0.05). Compared with the normal group and the sham operation group, the activity of ChAT in the cortex and hippocampus decreased significantly (P0.01).
Compared with the normal group and the sham operation group, the AchE activity of the cortex and hippocampus of the model group was significantly lower than that of the normal group and the sham group (P0.01). Compared with the model group, the activity of the cortex and hippocampus of the model group was significantly increased (P0.01), and there was no significant difference between the western medicine group and the moxibustion group (P0.05), compared with the normal group and the sham operation group (P0.01). There was no significant difference between the sham operation group and the normal group (P0.05).
4. effect of Mongolian medicine moxibustion on Bcl-2 and Bax: mRNA and protein expression in hippocampus of AD rats
(1) the mRNA and protein expression of Bcl-2 in hippocampus of rats in each group, compared with the normal group and sham operation group, the mRNA and protein expression level of Bcl-2 in the model group decreased significantly (P0.01). Compared with the model group, the level of mRNA and protein expression of Bcl-2 was significantly increased (P0.01, P0.05), and there was no significant difference between the western medicine group and the moxibustion group. Difference (P0.05); there was no significant difference between sham operation group and normal group (P0.05).
(2) compared with the normal group and the sham operation group, the expression of mRNA and protein of Bax in the model group was significantly higher than that of the normal group and the sham group (P0.01). The level of mRNA and protein expression of Bax in the moxibustion group and the western medicine group were significantly lower than that of the model group (P0.01). There was no significant difference between the western medicine group and the moxibustion group (P0.01). (2) the expression of mRNA and protein in the model group was significantly higher than that of the model group (P0.01). P0.05); there was no significant difference between the sham operation group and the normal group (P0.05).
conclusion
1. the rat model of Alzheimer's disease was established by subcutaneous injection of D- galactose and bilateral hippocampal injection of A beta 25-35 in rats. It could well simulate the behavioral and pathological features of AD learning and memory impairment, neuron injury, neural cell apoptosis and so on. It is closer to the pathological appearance of AD, and has good localization and practicability.
2. Mongolian medicine moxibustion therapy can improve the behavioral function of AD rats, improve learning and memory ability, and prevent and cure AD.
3. Mongolian medicine moxibustion therapy can reduce the damage of nerve cells in the hippocampus of AD rats, inhibit the apoptosis of nerve cells, and prevent and cure AD.
4. Mongolian medicine moxibustion can improve the activity of SOD in the brain tissue of AD rats, reduce the content of MDA, reduce the lipid peroxidation, improve the activity of antioxidant enzymes, remove the free radicals and resist senescence, thus preventing and developing the occurrence and development of AD disease.
5. Mongolian medicine moxibustion can improve the activity of ChAT and AchE in the brain tissue of AD rats, protect the activity of brain enzymes and improve the energy metabolism disorder of brain tissue, which may improve the dysfunction of the cholinergic system of AD.
6. Mongolian medicine moxibustion therapy has an up-regulated effect on the mRNA and protein of Bcl-2 in the hippocampus of AD rats. It has a downregulation effect on the mRNA and protein of Bax, and has a good inhibitory effect on the apoptosis of brain nerve cells of AD.
7. after the Mongolian medicine "Ding Hui point", "Xin point" and "stomach acupuncture point" in Mongolian AD, Mongolian medicine moxibustion therapy can improve learning and memory function.

【學(xué)位授予單位】：北京中醫(yī)藥大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2014
【分類號】：R29

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