本文選題：非酒精性脂肪肝　切入點(diǎn)：健脾清化方　出處：《河南中醫(yī)學(xué)院》2014年碩士論文

【摘要】：研究背景及目的：近年來，非酒精性脂肪性肝�。╪on-alcoholic fatty liverdisease， NAFLD）已成為一種影響我國人民健康的常見病。在肝細(xì)胞內(nèi)以甘油三酯為主的脂質(zhì)大量的堆積是本病的特征。人體內(nèi)中性脂肪分解產(chǎn)生的游離脂肪酸（free fatty acid，F(xiàn)FA）經(jīng)由肝臟攝取，在肝細(xì)胞內(nèi)參與脂質(zhì)的合成，各種原因引起的FFA含量增多，造成肝細(xì)胞內(nèi)脂質(zhì)過度沉積，從而引發(fā)NAFLD。本實(shí)驗(yàn)通過觀察健脾清化方對(duì)NAFLD大鼠血清游離脂肪酸（free fatty acid，F(xiàn)FA），肝內(nèi)甘油三酯（triglyceride，TG）的影響，研究健脾清化方“健脾清熱化濕”治法改善脂代謝、治療脂肪肝的確切療效。 方法：選用健康雄性SD大鼠50只，適應(yīng)性喂養(yǎng)一周后，采用隨機(jī)數(shù)字表。隨機(jī)抽取8只為正常組，，給予基礎(chǔ)飼料，飲用自來水；其余為高脂組，參考文獻(xiàn)[1]給予高脂飼料（84%普通飼料+10%豬油+5%蛋黃粉+1%膽固醇），飲用自來水。10周后，隨機(jī)從造模組和正常組各抽取2只，用水合氯醛麻醉，處死，取肝臟做病理切片，驗(yàn)證造模成功。再用隨機(jī)數(shù)字表法將高脂組分為模型對(duì)照組、健脾清化方高、低劑量組，東寶肝泰組，正常組和模型組灌服生理鹽水，各組共給藥8周。除正常組外，各組繼續(xù)以高脂飼料喂養(yǎng)。第18周處死各組大鼠，方法為隔夜禁食不禁水，次日稱量體重后，以水合氯醛腹腔注射麻醉，從腹主動(dòng)脈采血，測定血清谷丙轉(zhuǎn)氨酶（alanine aminotransferase，ALT）、谷草轉(zhuǎn)氨酶（abstract syntax tree，AST），取肝組織，稱重，制備肝勻漿及肝組織切片，檢測肝組織FFA、TG含量，光鏡下觀察肝組織病理變化。 結(jié)果：通過測定各組大鼠給藥后肝組織FFA、TG含量的變化，結(jié)果表明模型組大鼠肝臟FFA、TG均顯著高于空白組（P0.05,）；健脾清化方高、低劑量組及東寶肝泰組對(duì)于降低NAFLD大鼠肝內(nèi)游離脂肪酸(FFA)、TG水平與模型組相比，均有顯著差異（P0.05）；健脾清化方高、低劑量組與東寶肝泰組FFA、TG無顯著差異（P0.05） 結(jié)論：1.健脾清化方可以降低NAFLD模型大鼠肝組織中FFA、TG濃度，減輕肝細(xì)胞脂變程度； 2.健脾清化方可以降低NAFLD模型大鼠血清ALT、AST水平。說明健脾清化方有保肝降酶作用。
[Abstract]:Background and purpose: in recent years, Non-alcoholic fatty liver disease (NAFLDD) has become a common disease affecting the health of Chinese people. The accumulation of triglycerides in liver cells is the characteristic of the disease. Free fatty acido FFAs were taken through the liver. Taking part in the synthesis of lipids in hepatocytes, the content of FFA increased in various reasons, resulting in excessive deposition of lipids in hepatocytes. The effect of Jianpi Qinghua recipe on serum free fatty acid free fatty acidine FFAA and triglyceride triglyceride in liver of NAFLD rats was observed in order to study the effect of Jianpi Qinghua recipe on improving lipid metabolism and treating fatty liver by "invigorating spleen and clearing heat and removing dampness". Methods: fifty male Sprague-Dawley (SD) rats were selected, fed adaptively for one week, then randomly selected as normal group (n = 8), fed with basic feed and drinking tap water, the rest were treated with high fat group. Reference [1] was given a high-fat diet of 84% lard 5% egg yolk powder 1% cholesterol, drinking tap water for 10 weeks. Two rats were randomly selected from the model group and the normal group. The rats were anesthetized with chloral hydrate and killed, and the liver was taken for pathological section. Then the high fat group was divided into three groups: the model control group, the high and low dose group, the Dongbao Gantai group, the normal group and the model group. The rats were given normal saline for 8 weeks, except the normal group. The rats in each group were killed at the 18th week. The rats were killed by fasting overnight. After weighing their weight the next day, the rats were anesthetized by chloral hydrate intraperitoneally, and blood was collected from the abdominal aorta. Serum alanine aminotransferase (alanine aminotransferase) and syntax treedosum (ASTT) were measured, liver tissue was taken, weighed, liver homogenate and liver tissue sections were prepared, liver tissue FFA-TG content was detected and pathological changes of liver tissue were observed under light microscope. Results: the changes of FFA-TG content in liver tissue of rats in each group were determined. The results showed that the FFA-TG in the model group was significantly higher than that in the blank group (P 0.05), and the content of Jianpi Qinghua prescription was higher in the model group than that in the blank group. There was significant difference between the low dose group and the Dongbao Gantai group in reducing the level of FFAA TG in the liver of NAFLD rats compared with the model group (P 0.05), but there was no significant difference between the low dose group and the Dongbao Gantai group in the level of FFAF TG (P0.05), the high level of Jianpi Qinghua recipe, and the low dose group and the Dongbao Gantai group (P 0.05). Conclusion 1. Jianpi Qinghua prescription can reduce the concentration of FFA-TG in liver tissue of NAFLD model rats, and alleviate the degree of hepatocyte lipid change. 2. Jianpi Qinghua prescription can reduce the serum alt level of NAFLD model rats, which indicates that Jianpi Qinghua prescription has the effect of protecting liver and lowering enzyme.
【學(xué)位授予單位】：河南中醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R285.5

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