本文選題：動(dòng)脈粥樣硬化　切入點(diǎn)：沙棘黃酮　出處：《青海大學(xué)》2014年碩士論文

【摘要】：目的：通過(guò)復(fù)制大鼠動(dòng)脈粥樣硬化病理模型，探討沙棘黃酮(TFH)對(duì)動(dòng)脈粥樣硬化(AS)大鼠血三脂和LDL-C、主動(dòng)脈病理改變及SOD、MDA的影響，并測(cè)定動(dòng)脈粥樣硬化大鼠血管中NADPH氧化酶亞基NOX4和P47phox蛋白表達(dá)含量，探討沙棘黃酮保護(hù)動(dòng)脈粥樣硬化大鼠血管的可能的分子機(jī)制。 方法：采用50只健康清潔級(jí)、雄性SD鼠，隨機(jī)分為5組：正常對(duì)照組，動(dòng)脈粥樣硬化組和沙棘黃酮低、中、高劑量組。動(dòng)脈粥樣硬化組和沙棘黃酮不同劑量組采用高脂飼料并腹腔注射維生素D3的方法來(lái)復(fù)制大鼠粥樣硬化模型，正常對(duì)照組則繼續(xù)飼喂普通飼料，沙棘黃酮不同劑量組自造模開(kāi)始后每日分別以沙棘黃酮50mg/kg、100mg/kg、200mg/kg定量喂食。8周后，在大鼠禁食12小時(shí)后麻醉大鼠，從腹主動(dòng)脈插管提純血清后采用全自動(dòng)生化分析儀測(cè)定血三脂和LDL-C含量,比色法測(cè)定SOD及MDA含量；處死大鼠后采用主動(dòng)脈HE染色來(lái)觀察主動(dòng)脈病理形態(tài)學(xué)變化，余主動(dòng)脈采用Elisa方法檢測(cè)其NADPH氧化酶亞單位NOX4和P47phox蛋白的含量變化。 結(jié)果：與正常對(duì)照組相比動(dòng)脈粥樣硬化組大鼠血脂表達(dá)紊亂，與動(dòng)脈粥樣硬化組比較，沙棘黃酮不同劑量組HDL-C的含量顯著升高，TG、TC、LDL-C等含量明顯減少，變化有統(tǒng)計(jì)學(xué)意義(P0.05)。與動(dòng)脈粥樣硬化組比較，沙棘黃酮不同劑量組SOD的表達(dá)含量明顯升高，MDA的表達(dá)顯著下降，變化有統(tǒng)計(jì)學(xué)意義(P0.05)。HE染色示動(dòng)脈粥樣硬化大鼠血管內(nèi)皮損傷，沙棘黃酮不同劑量組內(nèi)皮損傷有所緩解，脂質(zhì)沉積較少。與正常對(duì)照組比較，動(dòng)脈粥樣硬化組大鼠P47phox和NOX4蛋白表達(dá)含量升高，與動(dòng)脈粥樣硬化組比較，沙棘黃酮不同劑量組P47phox和NOX4蛋白表達(dá)顯著降低，差異有統(tǒng)計(jì)學(xué)意義(P0.01)。 結(jié)論：沙棘黃酮具有調(diào)節(jié)脂質(zhì)代謝，提高機(jī)體防御氧化應(yīng)激能力，阻止大鼠動(dòng)脈粥樣硬化進(jìn)展，，其機(jī)制可能與降低NADPH氧化酶蛋白表達(dá)有關(guān)。
[Abstract]:Objective: to replicate the model of atherosclerosis in rats, to investigate the Seabuckthorn flavonoids (TFH) on atherosclerosis (AS) rats blood lipid and LDL-C in three, aortic pathological changes and SOD, MDA, and vascular atherosclerosis rats NADPH oxidase subunit NOX4 and P47phox protein expression, investigate the possible molecular mechanism of flavonoids of seabuckthorn the protection of vascular atherosclerosis rats.
Methods: 50 healthy clean grade male SD rats, were randomly divided into 5 groups: normal control group, atherosclerosis group and seabuckthorn flavonoids, low and high dose group. Atherosclerosis group and different dose groups of seabuckthorn flavonoids using method of high fat diet and intraperitoneal injection of vitamin D3 to copy the model of atherosclerosis in rats, normal the control group was fed with normal diet to different dose groups, seabuckthorn flavonoids from the model after the start of day respectively by 100mg/kg, 200mg/kg 50mg/kg of seabuckthorn flavonoids, quantitative feeding after.8 weeks, the rats in the rats were fasted for 12 hours after anesthesia, purification of serum from abdominal aorta after intubation blood were measured by automatic biochemical analyzer and LDL-C three fat content determination of SOD, MDA and the content of colorimetry; rats were sacrificed after the aortic HE staining to observe pathological changes in the aorta, the aorta was detected by Elisa more than the NADPH oxidase subunit NOX The changes in the content of 4 and P47phox proteins.
Results: compared with normal control group the expression of atherosclerosis rats blood lipid disorder, compared with atherosclerosis group, the content of seabuckthorn flavonoids in different dosage group HDL-C significantly increased, TG, TC, LDL-C decreased significantly, with statistically significant difference (P0.05). Compared with the atherosclerosis group, the expression content of seabuckthorn flavonoids in different dosage group SOD the increased expression of MDA decreased significantly, with statistically significant difference (P0.05).HE staining showed atherosclerotic vascular endothelial injury in rats, the sea buckthorn flavone different dose groups of endothelial injury eased, lipid deposition is less. Compared with normal control group, atherosclerosis group, P47phox and NOX4 protein expression in rats was increased, compared with atherosclerosis group. The expression of P47phox and NOX4 protein in different dose groups of seabuckthorn flavonoids decreased significantly, the difference was statistically significant (P0.01).
Conclusion: Hippophae rhamnoides flavonoids can regulate lipid metabolism, improve the body's ability to prevent oxidative stress, and prevent progression of atherosclerosis in rats, which may be related to the reduction of NADPH oxidase protein expression.

【學(xué)位授予單位】：青海大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R543.5

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