本文選題：降逆和胃湯　切入點(diǎn)：膽汁反流性胃炎　出處：《遼寧中醫(yī)藥大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：本實(shí)驗(yàn)是采用反流液來復(fù)制大鼠膽汁反流性胃炎動物模型，在造模成功后對其進(jìn)行藥物治療，通過降逆和胃湯對反流性胃炎大鼠動物模型一般狀態(tài)，血清白介素-6（LI-6）、腫瘤壞死因子（TNF-a）、胃泌素（GAS）、內(nèi)皮素（ET）水平，胃黏膜組織病理學(xué)改變，并與鋁碳酸鎂片進(jìn)行對比，闡明降逆和胃湯防治大鼠膽汁反流性胃炎胃黏膜損傷的作用機(jī)制。 材料與方法： 1分組造模與干預(yù)治療 健康雌雄Wister實(shí)驗(yàn)大鼠共72只，在實(shí)驗(yàn)室自然采光下，適應(yīng)性喂養(yǎng)3天。根據(jù)大鼠的體重不同，將符合條件的大鼠隨機(jī)分為了6組：空白組、模型組、降逆和胃湯高、中、低劑量組及西藥陽性對照組，按大鼠性別雌雄各半。除空白組給予等量蒸餾水灌胃外，其余5組大鼠均連續(xù)向胃內(nèi)灌反流液進(jìn)行造模15d，反流液配制以胰酶1．5g、�；悄懰徕c2．5g、孵磷脂0．25g(奧博星)三種溶于100ml的蒸溜水之中，并以1．5ml／lOOg灌胃，日2次。造模結(jié)束以后，模型組和空白組繼續(xù)灌胃同樣給予等容量的生理鹽水，西藥陽性對照組予鋁碳酸鎂片。降逆和胃湯高、中、低組劑量分別為37.2g、18.6、9.3生藥/kg體重。 2一般狀況的觀察 在實(shí)驗(yàn)過程中，對大鼠的精神狀態(tài)、飲食、二便情況及反流液灌胃后體重、毛色和死亡等情況變化進(jìn)行詳細(xì)的記錄觀察。 3實(shí)驗(yàn)指標(biāo)取材及檢測 灌胃30天后各組大鼠進(jìn)行取材:最后一次灌胃結(jié)束，禁食禁水12小時(shí)。取材當(dāng)天對大鼠先進(jìn)行稱重，按體重向腹腔內(nèi)注射麻醉，每只腹主動脈取血2ml，用做血清白介素-6、腫瘤壞死因子-a、胃泌素、內(nèi)皮素水平的測定。取血完畢，無菌操作下取胃。用剪刀沿胃的大彎處剪開，胃中內(nèi)容物拿冰生理鹽水洗清，再用濾紙將胃組織吸干，，將其放入多聚甲醛溶液里面以固定，進(jìn)行電子顯微鏡下觀察胃黏膜的病理學(xué)改變。 結(jié)果： 第一部分：降逆和胃湯對膽汁反流性胃炎模型大鼠血清中LI-6、TNF-a、GAS、ET-2含量的影響 各組大鼠血清IL-6、GAS、TNF-a、ET含量的比較。與模型組相比較,降逆和胃湯高、中、低劑量組與西藥陽性對照組均可提高IL-6、GAS、TNF-a，降低ET的含量，均具有統(tǒng)計(jì)學(xué)意義（P0．05)，與西藥陽性對照組相比較，和胃降逆湯高劑量組IL-6、TNF-a、GAS、ET未有明顯變化：差異無統(tǒng)計(jì)學(xué)意義(P0．05)。 第二部分：降逆和胃湯對膽汁反流性胃炎模型大鼠胃黏膜病理學(xué)上的影響空白組中的大鼠胃黏膜呈現(xiàn)出光滑、無脫離及破損，很少能看到炎癥細(xì)胞的出現(xiàn)。模型組中的大鼠胃黏膜則出現(xiàn)脫離和破損，可見大量的炎癥細(xì)胞。在與模型組的比較中，降逆和胃湯高、中、低劑量各組和西藥陽性對照組胃黏膜細(xì)胞的脫離和破損比較輕，炎癥細(xì)胞的浸潤比較少，降逆和胃湯高劑量組效果最佳，其次是中劑量和西藥陽性對照組。對半定量指標(biāo)所顯示出的炎癥細(xì)胞結(jié)果：較模型組，降逆和胃湯高、中、低劑量各組與西藥陽性對照組中的炎癥細(xì)胞浸潤明顯減輕（P0.05）。 結(jié)論： 1降逆和胃湯對膽汁反流性胃炎大鼠的白細(xì)胞介素-6、腫瘤壞死因子-a、胃泌素含量顯著的升高，內(nèi)皮素含量降低。加強(qiáng)了胃黏膜的保護(hù)作用，減少對胃黏膜的損傷。 2在治療膽汁反流性胃炎用降逆和胃湯，可減少炎癥細(xì)胞的浸潤，能改善膽汁反流性胃炎模型大鼠胃黏膜病理學(xué)的變化，擁有保護(hù)胃黏膜，減少胃黏膜損傷的作用。
[Abstract]:Objective: the purpose of this experiment is the use of reflux liquid in rats with bile reflux gastritis animal model, the drug therapy in after the success of the model, through Jiangni Hewei decoction on reflux gastritis rat animal model of general condition, serum interleukin -6 (LI-6), tumor necrosis factor (TNF-a), gastrin (GAS), endothelin (ET) levels, pathological changes of gastric mucosa pathology, and compared with Hydrotalcite Tablets, and clarify Jiangni Decoction in Treating Gastric Bile Reflux Gastritis in rats with gastric mucosal injury mechanism.
Materials and methods:
1 group modeling and intervention therapy
Healthy male Wister rats 72 only, in the natural lighting laboratory, adaptive feeding for 3 days. According to the body weight of rats, the rats were randomly divided in order to meet the conditions of the 6 groups: blank group, model group, jiangnihewei dose, and low dose group and positive medicine control group, according to the rat sex female. In addition to the control group given distilled water by gavage, the remaining 5 rats were intragastric perfusion to continuous reflux liquid molding 15d, reflux liquid prepared by sodium taurocholate trypsin 1.5g, 2.5G, 0.25g (aoboxing) with phospholipid three dissolved in 100ml distilled water, and the 1.5ml / lOOg orally, 2 times a day. After the end of the modeling, model group and blank group to gavage also received the equal volume of normal saline control group, positive medicine to Hydrotalcite Tablets. Jiangnihewei dose, low dose group, respectively 37.2g, 18.6,9.3 crude drug /kg weight.
2 observation of the general situation
During the experiment, the changes of the mental state, diet, two urine, weight, hair color and death of the reflux fluid were recorded and observed in detail.
3 experimental indexes and testing
30 days after the gavage rats were collected: the last gavage end, fasting for 12 hours. Based on the day of the rats were weighed according to the weight to the intraperitoneal injection of anesthesia, each abdominal aortic blood 2ml, with serum interleukin -6, tumor necrosis factor -a, gastrin, determination of endothelial on level of blood. After aseptic operation the stomach. The stomach big bend along with scissors cut, with ice saline wash contents in the stomach, and the stomach tissue paper dry, put it into the poly Formaldehyde Solution inside a fixed, the pathological changes of gastric mucosa was observed by electron microscope.
Result錛

本文編號：1642548