本文選題：壯骨止痛膠囊　切入點(diǎn)：雌激素　出處：《時(shí)珍國(guó)醫(yī)國(guó)藥》2017年04期 　論文類型：期刊論文

【摘要】：目的通過檢測(cè)壯骨止痛膠囊不同治法配伍對(duì)去卵巢骨質(zhì)疏松大鼠骨組織E_2和ER_β的影響,探討壯骨止痛膠囊的治則組方機(jī)理,為臨床治療絕經(jīng)后骨質(zhì)疏松癥的配伍組方提供指導(dǎo)。方法根據(jù)壯骨止痛膠囊補(bǔ)益肝腎、活血止痛治法將處方拆方分為補(bǔ)陽組、補(bǔ)陰組、活血組、陰陽雙補(bǔ)組、活血補(bǔ)陽組、活血補(bǔ)陰組。90只8月齡SD雌鼠隨機(jī)分為假手術(shù)組、模型組、壯骨止痛膠囊組、補(bǔ)陽組、補(bǔ)陰組、活血組、陰陽雙補(bǔ)組、活血補(bǔ)陽組、活血補(bǔ)陰組,每組10只。假手術(shù)組大鼠保留卵巢而只去除卵巢附近部分脂肪組織,其余各組雌鼠雙側(cè)去卵巢復(fù)制絕經(jīng)后骨質(zhì)疏松動(dòng)物模型,術(shù)后1周灌胃給藥18周,最后1次給藥1 h后腹腔注射水合氯醛麻醉(0.35ml/100g),腹主動(dòng)脈放血處死,取左股骨檢測(cè)E_2水平,取右股骨檢測(cè)骨密度和骨生物力學(xué),快速取右脛骨RT-PCR檢測(cè)ER_βmRNA的表達(dá),取左脛骨病理切片免疫組化染色檢測(cè)ER_β蛋白水平。結(jié)果活血組對(duì)骨密度沒有顯著影響,但能顯著增強(qiáng)骨生物力學(xué),其它配伍組均能顯著增加骨密度和改善骨生物力學(xué)(P0.05或P0.01),其改善作用從高到低依次為全方組陰陽雙補(bǔ)組活血補(bǔ)陽組活血補(bǔ)陰組補(bǔ)陽組補(bǔ)陰組活血組。除活血組外的其它各配伍組均顯著提高模型大鼠骨組織E_2水平(P0.05或P0.01)。所有配伍組能顯著增強(qiáng)骨組織ER_β的基因和蛋白表達(dá)(P0.05或P0.01),作用依次為全方組陰陽雙補(bǔ)組活血組活血補(bǔ)陽組活血補(bǔ)陰組補(bǔ)陽組補(bǔ)陰組。結(jié)論壯骨止痛膠囊中的活血組藥物主要增強(qiáng)骨組織對(duì)雌激素的敏感性,補(bǔ)陽組和補(bǔ)陰組藥物主要促進(jìn)機(jī)體合成雌激素,提高骨組織的雌激素水平,同時(shí)提高骨組織的雌激素敏感性。治療絕經(jīng)后骨質(zhì)疏松治法以補(bǔ)益肝腎為主,輔以活血化瘀,有助于取得更好療效。
[Abstract]:Objective to investigate the mechanism of Zhuanggu Zhitong capsule (ZZC) by detecting the effect of Zhuanggu Zhitong capsule (Zhuanggu Zhitong capsule) on the bone tissue of ovariectomized rats. Methods according to the prescription of Zhuanggu Zhitong capsule tonifying liver and kidney, activating blood circulation and relieving pain, the prescription was divided into three groups: group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B, group B. Eight-month-old SD female rats were randomly divided into sham-operation group, model group, Zhuanggu Zhitong capsule group, tonifying yang group, invigorating yin group, activating blood circulation group, yin and yang reinforcing group, activating blood circulation and reinforcing yang group, activating blood circulation and nourishing yin group, promoting blood circulation and nourishing yin group. 10 rats in each group: rats in sham-operation group kept their ovaries and only removed some adipose tissue near their ovaries. The other female rats were ovariectomized to make postmenopausal osteoporosis animal model. The rats were given orally for 18 weeks 1 week after operation. One hour after the last administration of chloral hydrate, 0.35 ml / 100 g of chloral hydrate was injected intraperitoneally, and the abdominal aorta was put to death. The left femur was taken to detect the E2 level, the right femur to detect bone mineral density and bone biomechanics, and the right tibia RT-PCR to detect the expression of ER尾 mRNA. The level of ER尾 protein was detected by immunohistochemical staining in pathological sections of left tibia. Results the blood activating group had no significant effect on bone mineral density, but could significantly enhance bone biomechanics. Other compatibility groups can significantly increase bone mineral density and improve bone biomechanics (P0.05 or P0.01). The order of improvement from high to low is the whole prescription group, yin and yang group, activating blood circulation and reinforcing yin group, activating yin group, activating blood circulation group, activating blood circulation group, activating blood circulation group, activating blood circulation group, activating blood circulation group, activating blood circulation group, activating blood circulation group, activating blood circulation group, activating blood circulation group, and activating blood circulation group. All the compatibility groups could significantly enhance the expression of ER尾 gene and protein in bone tissue of model rats by P0.05 or P0.010.The effect was in the order of activating blood circulation and reinforcing yang in the whole prescription group with both yin and yang invigorating blood circulation group. Conclusion the drugs in Zhuanggu Zhitong capsule mainly enhance the sensitivity of bone tissue to estrogen. The drugs mainly promote the synthesis of estrogen, increase the estrogen level of bone tissue, and increase the sensitivity of estrogen in bone tissue. The treatment of postmenopausal osteoporosis is mainly to invigorate liver and kidney, and to promote blood circulation and remove blood stasis. It is helpful to obtain better curative effect.
【作者單位】： 湖南中醫(yī)藥大學(xué)第一附屬醫(yī)院;中南大學(xué)湘雅藥學(xué)院;
【基金】：國(guó)家自然科學(xué)基金面上項(xiàng)目(No.81273816)
【分類號(hào)】：R285.5

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