本文選題：肝硬化　切入點：胰島素樣生長因子結(jié)合蛋白質(zhì)　出處：《中華消化病與影像雜志(電子版)》2014年04期 　論文類型：期刊論文

【摘要】：目的探討腺病毒包裝的胰島素樣生長因子結(jié)合蛋白相關(guān)蛋白1(Ad-IGFBPrP1)對大鼠肝組織中NF-κB p65的表達影響及意義。方法 32只健康雄性SD大鼠隨機分為4組,每組8只。正常對照組:尾靜脈注射生理鹽水;陰性對照Ad-EGFP組:尾靜脈注射腺病毒包裝的增強型綠色熒光蛋白(Ad-EGFP);Ad-IGFBPrP1 2周組:尾靜脈注射Ad-IGFBPrP1;Ad-IGFBPrP1 4周組:尾靜脈注射Ad-IGFBPrP1。分別于注射2周、4周末乙醚麻醉大鼠,取肝左葉固定、包埋、切片。行HE染色,觀察肝組織病理改變;苦味酸-天狼星紅染色觀察肝組織中膠原纖維含量的變化;免疫組織化學(xué)染色觀察IGFBPrP1、核因子κBp65(NF-κBp65)、α-平滑肌肌動蛋白(α-SMA)、纖維連接蛋白(Fn)的表達及分布。結(jié)果 HE和苦味酸-天狼星紅染色顯示:與正常對照組、陰性對照Ad-EGFP組的正常肝組織相比,Ad-IGFBPrP1組肝組織出現(xiàn)病理改變且發(fā)生纖維化(P0.01)。免疫組織化學(xué)染色顯示:Ad-IGFBPrP1組肝組織中IGFBPrP1、NF-κB p65、α-SMA、Fn的表達較正常對照組、陰性對照Ad-EGFP組明顯增強(P0.01);Ad-IGFBPrP1 4周組的表達高于2周組(P0.01)。結(jié)論 Ad-IGFBPrP1經(jīng)尾靜脈注射成功轉(zhuǎn)染進入大鼠,引起肝組織中NF-κB p65表達增高,最終導(dǎo)致肝組織纖維化,提示IGFBPrP1致肝纖維化的機制之一可能通過NF-κB信號轉(zhuǎn)導(dǎo)通路實現(xiàn)。
[Abstract]:Objective to investigate the effect and significance of adenovirus-packaged insulin-like growth factor binding protein-associated protein (1Ad-IGFBPrP1) on the expression of NF- 魏 B p65 in rat liver. Methods 32 healthy male SD rats were randomly divided into 4 groups. 8 rats in each group. Normal control group: normal saline was injected into tail vein; Ad-EGFP group: Ad-EGFBPrP1 group: Ad-IGFBPrP1 group: Ad-IGFBPrP1 group: Ad-IGFBPrP1 group: Ad-IGFBPrP1 group: Ad-IGFBPrP1 group: Ad-IGFBPrP1 group: Ad-IGFBPrP1 group: Ad-IGFBPrP1 group. The pathological changes of liver tissue were observed by HE staining and the changes of collagen fibers in liver tissue were observed by picric acid-Sirius red staining. Immunohistochemical staining was used to observe the expression and distribution of IGFBPrP1, NF- 魏 Bp65, NF- 魏 Bp65, 偽 -smooth muscle actin (偽 -SMAN). Results HE and picric acid-Sirius red staining showed that the expression and distribution of 偽 -smooth muscle actin (偽 -SMAN) were similar to those of normal controls. Compared with Ad-IGFBPrP1 group, the normal liver tissue of the negative control group showed pathological changes and fibrosis P0.01.The immunohistochemical staining showed that the expression of IGFBPrP1- 魏 B p65 and 偽 -SMAFN in the liver tissue of the control group was higher than that of the normal control group. The expression of P0.01- IGFBPrP1 in the negative control Ad-EGFP group was significantly higher than that in the 2-week group. Conclusion the expression of NF- 魏 B p65 in liver tissue was increased and the liver fibrosis was induced by successful transfection of Ad-IGFBPrP1 through tail vein into rats. The results suggest that one of the mechanisms of hepatic fibrosis induced by IGFBPrP1 may be achieved by NF- 魏 B signal transduction pathway.
【作者單位】： 山西醫(yī)科大學(xué)第一醫(yī)院消化科;山西醫(yī)科大學(xué)第一醫(yī)院科研實驗中心;
【基金】：山西省回國留學(xué)人員重點科研資助項目(2012-重點4) 太原市科技明星項目(120247-04)
【分類號】：R575

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