本文選題：高滲鹽　切入點(diǎn)：燒傷　出處：《安徽醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:本實(shí)驗(yàn)通過采用600mmol/L、800mmol/L、1000mmol/L三種Na+濃度高滲鈉鹽液(HS)和乳酸格林鈉液(LR)對重度燙傷大鼠進(jìn)行液體復(fù)蘇,從肝臟功能,肝組織細(xì)胞形態(tài),炎癥反應(yīng),氧化應(yīng)激,炎癥相關(guān)信號轉(zhuǎn)導(dǎo)通道蛋白等方面探討不同高滲鈉鹽液(HS)對大鼠重度燙傷后肝臟損傷的影響。方法:健康成年雌性SD大鼠104只,體重200-250g,隨機(jī)分成五組:假傷組(8只)、其余為燙傷組包括LR組(24只)、600HS組(24只)、800HS組(24只)、1000HS組(24只)。大鼠麻醉后扎套管針尾靜脈置管,剃去背部毛發(fā),假傷組大鼠不予燙傷及補(bǔ)液,擬傷后直接取腹主動脈血及肝組織;其余各組均給予大鼠背部Ⅱ°至Ⅲ°開水燙傷,傷后分別使用LR復(fù)蘇和三種不同HS復(fù)蘇。燙傷組大鼠于補(bǔ)液后2h、8h和24h取腹主動脈血及肝組織。第一部分:不同鈉離子濃度高滲鹽復(fù)蘇液對重度燙傷大鼠肝功能損傷及肝細(xì)胞損傷的影響取出五組大鼠的一部分肝臟組織及血漿,使用自動生化儀檢測血漿中ALT及AST含量,肝臟組織在使用10%甲醛固定后使用HE染色法染色觀察組織細(xì)胞形態(tài)。第二部分:不同鈉離子濃度高滲鹽復(fù)蘇液對重度燙傷大鼠肝臟氧化損傷及炎癥因子的影響取出五組大鼠的一部分肝臟組織及血漿,比色法對肝組織進(jìn)行丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性測定。酶聯(lián)免疫吸附法(ELISA)測量血漿中腫瘤壞死因子α(TNF-α)、白介素1β(IL-1β)以及高遷移率族蛋白B1(HMGB-1)。將取得數(shù)據(jù)進(jìn)行統(tǒng)計(jì)分析。第三部分:不同鈉離子濃度高滲鹽復(fù)蘇液對重度燙傷大鼠肝臟組織p38MAPK及JNK通道活化的影響使用蛋白質(zhì)印跡法(Western Blot)測量五組大鼠肝臟組織中p38MAPK以及JNK通道磷酸化蛋白比值并進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果:第一部分與假傷組相比,傷后各時(shí)間點(diǎn),其余各組ALT、AST均明顯升高(P0.01);與LR組相比,傷后各時(shí)間點(diǎn),600HS組ALT、AST均明顯降低,(P0.01),而800HS組及1000HS組ALT、AST在傷后2、8小時(shí)未見顯著變化(P0.05),在傷后24小時(shí)800HS組及1000HS組ALT、AST均明顯降低(P0.01);與600HS組相比,800HS組及1000HS組ALT、AST在傷后2、8小時(shí)明顯升高(P0.01),在傷后24小時(shí),800HS組及1000HS組ALT、AST未見顯著差異(P0.05)。肝臟組織HE染色顯示:傷后各時(shí)間點(diǎn),LR組大鼠肝細(xì)胞可見明顯脂肪樣變,部分可見細(xì)胞水腫;傷后2、8小時(shí)HS組大鼠隨高滲鹽濃度升高,上述病理變化增多;傷后24小時(shí),三組HS組大鼠肝臟病理變化無明顯差異。第二部分與假傷組相比,傷后各時(shí)間點(diǎn),LR組、800HS組和1000HS組TNF-α與IL-1β均明顯升高(P0.01),而600HS組未見明顯變化(P0.05);與LR組相比,傷后各時(shí)間點(diǎn)600HS組TNF-α與IL-1β均明顯降低(P0.01),800HS組和1000HS組未見顯著變化(P0.05)。傷后2小時(shí),五組HMGB-1無顯著差異(P0.05),傷后8、24小時(shí),與假傷組相比,LR組、800HS組和1000HS組HMGB-1均明顯升高(P0.01),而600HS組未見明顯差異(P0.05);與LR組相比,各HS組HMGB-1均明顯降低(P0.01);與600HS組相比,800HS組及1000HS組HMGB-1明顯升高(P0.01)。與假傷組相比,傷后各時(shí)間點(diǎn)其余各組MDA含量明顯升高SOD活性明顯降低(P0.01);與LR組相比,傷后各時(shí)間點(diǎn)600HS組MDA含量明顯降低SOD活性明顯升高(P0.01),800HS組、1000HS組未見顯著變化(P0.05);與600HS組相比,傷后各時(shí)間點(diǎn)800HS組及1000HS組MDA含量明顯升高SOD活性明顯降低(P0.01)。第三部分肝臟組織p38MAPK以及JNK通道磷酸化蛋白比值顯示:LR組和各HS組在傷后2h、8h及24h,樣本肝臟組織p38MAPK及JNK通道的活性表達(dá)均比假傷組顯著升高(P0.01);而LR組、800HS組及1000HS組p38MAPK及JNK通道活性表達(dá)均顯著高于600HS組(P0.01),而800HS組及1000HS組p38MAPK及JNK通道活性與LR組無差異(P0.05)。結(jié)論:(1)與傷后使用LR相比,使用600mmol/L鈉離子濃度的高滲鹽溶液進(jìn)行液體復(fù)蘇能減輕但不終止大鼠的肝功能失常及肝細(xì)胞形態(tài)結(jié)構(gòu)的損傷,但是隨著Na+濃度升高達(dá)到800及1000mmol/L后,效果降低。(2)與傷后使用LR相比,使用600mmol/L鈉離子濃度的高滲鹽溶液進(jìn)行液體復(fù)蘇能減輕并部分終止重度燙傷大鼠傷后肝臟的氧化損傷以及炎癥反應(yīng)發(fā)生,但是隨著Na+濃度升高,達(dá)到800及1000mmol/L后,效果降低。(3)與傷后使用LR相比,使用600mmol/L鈉離子濃度的高滲鹽溶液進(jìn)行液體復(fù)蘇能減輕但不終止肝臟組織中p38MAPK和JNK通道的激活表達(dá),但是隨著Na+濃度升高,達(dá)到800及1000mmol/L后,效果降低。
[Abstract]:Objective: this experiment by using 600mmol/L, 800mmol/L, 1000mmol/L three Na+ concentration of hypertonic salt solution (HS) and Green (LR) lactic acid liquid sodium on Severe Scalded Rats by fluid resuscitation from the liver function, liver cell morphology, inflammation, oxidative stress, inflammation related signaling channel protein of different hypertonic salt solution (HS) of rats after severe burns after liver injury. Methods: 104 healthy adult female SD rats, weighing 200-250g, were randomly divided into five groups: sham injury group (8 rats), the rest of the scald group including LR group (24 rats), 600HS group (24), 800HS group (24 rats), 1000HS group (24 rats). Rats were anesthetized with intravenous trocar catheter, shaved back hair, the sham injury group rats with scald and rehydration, to directly after the injury abdominal aorta blood and liver tissue; the other groups were given back to the boiling water second-degree third degree in rats burns, LR respectively after injury recovery And three different HS recovery. Scald rats in group 8h and 24h after 2H infusion, abdominal aorta blood and liver tissue. The first part: different concentration of sodium ions of hypertonic saline resuscitation fluids on severe burn injury and liver function of liver cell injury in rats out of the five groups of rats a portion of the liver tissue and plasma the use of ALT, and the content of AST automatic biochemical detection of plasma and liver tissue using HE staining to observe cell morphology staining in 10% formalin fixed. The second part: the different concentration of sodium ions of hypertonic saline resuscitation fluid effects on liver oxidative damage and inflammatory factors in rats with severe burn out of the five groups of rats a portion of the liver tissue and plasma, colorimetry of liver tissue malondialdehyde (MDA) content, superoxide dismutase (SOD) activity assay. Enzyme linked immunosorbent assay (ELISA) in the measurement of plasma tumor necrosis factor alpha (TNF- alpha), interleukin 1 beta (IL-1 beta) to And the high mobility group protein B1 (HMGB-1). The obtained data were statistically analyzed. The third part: the sodium ion concentration of hypertonic saline resuscitation fluid using the method of severe scald rat liver tissue p38MAPK and JNK channel activation by Western blot (Western Blot) in liver tissue were measured in five groups of rats in p38MAPK and JNK channel phosphate the ratio of protein and analyzed statistically. Results: the first part compared with the sham injury group at each time point, the rest of groups ALT, AST were significantly increased (P0.01); compared with the LR group at each time point in group 600HS, ALT, AST were significantly lower (P0.01), and 800HS group 1000HS ALT AST group, there was no significant changes in 2,8 hours after injury (P0.05), 24 hours after injury in 800HS group and 1000HS group ALT, AST were significantly decreased (P0.01); compared with 600HS group, 800HS group and 1000HS group ALT, AST in 2,8 hours after injury was significantly increased (P0.01), after injury 24 hours, 800HS group 鍙，

本文編號：1580262