本文選題：呼吸機(jī)相關(guān)肺損傷　切入點：自噬　出處：《中國老年學(xué)雜志》2015年01期 　論文類型：期刊論文

【摘要】：目的探討大鼠呼吸機(jī)所致肺損傷(VILI)過程中自噬相關(guān)蛋白的表達(dá)及意義。方法健康雄性SPF級SD大鼠30只,7周齡,體重(220±20)g,實施麻醉和氣管切開術(shù)后分別接受不同潮氣量(VT)的通氣(通氣時間均為4 h)。隨機(jī)分為三組:對照組(A組,僅切開氣管保留自主呼吸);常規(guī)通氣組(B組,VT=10 ml/kg);損傷通氣組(C組,VT=40 ml/kg)。A組在氣管切開即刻,其他組在機(jī)械通氣結(jié)束后放血處死動物,Western印跡檢測肺組織Beclin1和LC3的表達(dá),計算肺濕/干比重(W/D比值)、MPO活性及肺泡灌洗液中總蛋白含量,光鏡下行白細(xì)胞計數(shù)。結(jié)果在大潮氣量機(jī)械通氣4 h后,肺組織中總蛋白、W/D、白細(xì)胞計數(shù)和MPO等指標(biāo)均顯著增加(P0.05);肺組織中Beclin1、LC3表達(dá)水平(1.21±0.11、0.91±0.11)均顯著增加。結(jié)論自噬表達(dá)的增加參與了大鼠VILI的發(fā)生。
[Abstract]:Objective to investigate the expression and significance of autophagy associated protein during ventilator induced lung injury in rats. Methods 30 healthy male SPF grade SD rats were aged 7 weeks. The body weight was 220 鹵20g. After anesthesia and tracheotomy, the ventilation time of different tidal volume (VT) was 4 h. The patients were randomly divided into three groups: group A, control group, and group A (control group). The lung tissue Beclin1 and LC3 expression were detected by Western blotting in group B (10 ml / kg), group C (group C) and group C (40 ml/kg).A) after tracheotomy, and the animals in other groups were sacrificed after mechanical ventilation, and the expression of Beclin1 and LC3 in lung tissue was detected by Western blotting. The activity of MPO and the content of total protein in alveolar lavage fluid were calculated. Total protein WR / D, white blood cell count and MPO were significantly increased in lung tissue, and the expression level of Beclin1 + LC3 was significantly increased in lung tissue. Conclusion the increased expression of autophagy may play an important role in the pathogenesis of VILI in rats.
【作者單位】： 鄭州大學(xué)第一附屬醫(yī)院胸外科;
【基金】：河南省教育廳科學(xué)技術(shù)重點研究項目(No.14A320076) 鄭州大學(xué)第一附屬醫(yī)院青年創(chuàng)新基金
【分類號】：R563.8

【共引文獻(xiàn)】

相關(guān)期刊論文 前1條

1 楊t熁，

本文編號：1563852