本文選題：初級(jí)軀體感覺皮層　切入點(diǎn)：癢覺　出處：《華東師范大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：癢是一種引起抓撓欲望的不愉快的感覺。目前,關(guān)于癢覺中樞機(jī)制的研究多采用腦功能成像的方法,這些研究雖然能讓我們看到腦內(nèi)有哪些腦區(qū)參與了癢覺信息的處理,但不能幫助我們了解這些腦區(qū)是如何對(duì)癢覺信息進(jìn)行處理的。腦成像研究對(duì)于初級(jí)軀體感覺皮層(Primary somatosensory cortex,SI)是否參與了癢覺信息的處理還存在爭(zhēng)論,本論文首次采用在體單細(xì)胞記錄的方法,從微觀層面上研究初級(jí)軀體感覺皮層是否參與癢覺信息的處理。本實(shí)驗(yàn)以wistar大鼠(10只,雄性,300-400克體重)作為實(shí)驗(yàn)動(dòng)物,手術(shù)將電極陣列埋置在雙側(cè)的SI,動(dòng)物恢復(fù)后,在戊巴比妥鈉麻醉狀態(tài)下,給予三種致癢劑(組胺、氯喹和五羥色胺)刺激,記錄初級(jí)軀體感覺皮層神經(jīng)元對(duì)不同致癢劑的反應(yīng),并記錄了這些神經(jīng)元對(duì)機(jī)械刺激和痛刺激(激光和辣椒素)的反應(yīng),以鑒定這些神經(jīng)元的性質(zhì)。對(duì)這些神經(jīng)元的放電信號(hào)進(jìn)行離線分析,觀察并比較SI神經(jīng)元對(duì)不同刺激的反應(yīng)模式。本研究記錄到了 51個(gè)SI神經(jīng)元,其中65%可以被組胺激活,而對(duì)五羥色胺和氯喹有響應(yīng)的神經(jīng)元比例低于組胺響應(yīng)神經(jīng)元的比例(分別為39%和29%);組胺和氯喹、五羥色胺激活的神經(jīng)元群體具有很大的重合;從不同致癢劑的反應(yīng)持續(xù)時(shí)間上來看,五羥色胺引起的反應(yīng)持續(xù)時(shí)間最長(zhǎng),其次是組胺和氯喹;大部分(92%)致癢劑響應(yīng)神經(jīng)元是廣動(dòng)力范圍神經(jīng)元,能被機(jī)械刺激或痛覺刺激所激活。綜上所述,我們的結(jié)果顯示在SI內(nèi)存在對(duì)致癢劑響應(yīng)的神經(jīng)元,并且絕大部分為多模態(tài)神經(jīng)元,非組胺依賴型致癢劑激活的可能是組胺響應(yīng)神經(jīng)元中的一個(gè)亞群,暗示在嚙齒類動(dòng)物的SI內(nèi)可能不存在分離的通路來處理組胺依賴和非組胺依賴的癢覺信息。本研究為SI參與癢覺信息的處理提供了直接證據(jù),將大大推進(jìn)我們對(duì)癢覺中樞機(jī)制的理解。
[Abstract]:Itching is an unpleasant feeling that causes scratching. At present, many studies on the mechanism of itch center use brain function imaging, although these studies can show us which brain regions are involved in the processing of itch information. But it doesn't help us understand how these brain regions process itching information. Brain imaging studies have debated whether primary somatosensory cortexSIs are involved in the processing of itch information. In this paper, we used the method of single cell recording in vivo for the first time to study whether the primary somatosensory cortex is involved in the processing of itch information at the microcosmic level. In this experiment, 10 wistar rats, male rats, 300-400g body weight) were used as experimental animals. The electrodes were implanted in bilateral SIs. After recovery, the animals were stimulated with three kinds of itching agents (histamine, chloroquine and serotonin) under pentobarbital sodium anesthesia. The responses of primary somatosensory cortical neurons to different itching agents were recorded. The responses of these neurons to mechanical and pain stimuli (laser and capsaicin) were recorded to identify the properties of these neurons. The response patterns of SI neurons to different stimuli were observed and compared. 51 SI neurons were recorded in this study, of which 65% could be activated by histamine. The proportion of neurons responding to serotonin and chloroquine was lower than that of histamine responsive neurons (39% and 29, respectively). According to the reaction duration of different itching agents, serotonin caused the longest reaction duration, followed by histamine and chloroquine; Which can be activated by mechanical or pain stimuli. In summary, our results show that there are neurons in SI that respond to itching agents, and most of them are multimodal neurons. Non-histamine-dependent itching agents may activate a subgroup of histamine-responsive neurons. It is suggested that there may not be separate pathways in rodent SI to process histamine-dependent and non-histamine-dependent itch information. This study provides direct evidence that SI is involved in the processing of itching information. This will greatly advance our understanding of the central mechanism of itching.
【學(xué)位授予單位】：華東師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：B842

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