本文關(guān)鍵詞： 高原低氧 增壓輔助 大鼠骨骼肌 HIF-1α 能量代謝　出處：《青海師范大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：通過建立大鼠在高原低氧環(huán)境下大強(qiáng)度運動后，將其轉(zhuǎn)移至增壓艙內(nèi)在0.2-0.3MPa（絕對壓力2-3ATA）的壓力環(huán)境中恢復(fù)的動物模型，探討高原低氧環(huán)境下大強(qiáng)度訓(xùn)練與施加增壓輔助方法對大鼠骨骼肌組織中HIF-1α、GLUT-1、GLUT-4蛋白表達(dá)及LDH、SDH酶活性的變化對大鼠骨骼肌疲勞恢復(fù)的影響。 方法：健康雄性Wistar大鼠56只隨機(jī)分為7組，每組8只：其中A組為安靜對照組，B組為自然恢復(fù)組，C組為0.2MPa增壓1h恢復(fù)組，D組0.2MPa增壓2h恢復(fù)組，E組為0.3MPa增壓1h恢復(fù)組，F(xiàn)組為低氧運動后自然恢復(fù)組，G組為低氧運動后0.2MPa增壓1h恢復(fù)組；平均體重A組197.71±16.87g，B組210.25±12.06g，C組208.38±4.75g，D組208.00±14.33g，E組210.88±11.99g，F(xiàn)組212.75±12.09g，G組210.50±8.53g。7組大鼠在西寧（2260m）青海大學(xué)醫(yī)學(xué)院動物實驗房進(jìn)行3天適應(yīng)性訓(xùn)練和6天正式訓(xùn)練:其中適應(yīng)期第1天跑速15m/min，其后每天遞增5m/min；適應(yīng)期坡度為0°；運動時間第一天15min，第二天20min，第三天30min。正式訓(xùn)練第1天跑速25m/min，第2天30m/min，以后4天維持此跑速至實驗結(jié)束；第1，2天坡度0°第3天增至10°，余下3天維持此坡度不變；正式訓(xùn)練期間運動時間60min/天。第7天進(jìn)行B組至G組速度為35m/mim、坡度為10°的力竭運動。運動后依照力竭運動組別順序除B組、F組外其余各組進(jìn)艙增壓恢復(fù)休息1h,增壓恢復(fù)結(jié)束后24h所有大鼠實施腹腔麻醉取一側(cè)腓腸肌，運用蛋白質(zhì)免疫印跡法檢測各組HIF-1α、GLUT-1、GLUT-4的蛋白表達(dá)，運用ELISA法檢測各組LDH和SDH酶活性。結(jié)果： （1）通過7天高原低氧環(huán)境下大強(qiáng)度運動后施加增壓輔助方法的恢復(fù)實驗發(fā)現(xiàn)，大鼠骨骼肌HIF-1α蛋白表達(dá)呈現(xiàn)先下降后上升再下降的趨勢，其中C組（0.2MPa增壓1h恢復(fù)）上升幅度較大。 （2）通過7天高原低氧環(huán)境下大強(qiáng)度運動后施加增壓輔助方法的恢復(fù)實驗發(fā)現(xiàn)，大鼠骨骼肌GLUT-1蛋白表達(dá)呈現(xiàn)先上升后下降再上升的趨勢，其中G組（低氧運動后0.2MPa的增壓1h恢復(fù)）上升較大。 （3）通過7天高原低氧環(huán)境下大強(qiáng)度運動后施加增壓輔助方法的恢復(fù)實驗發(fā)現(xiàn)，大鼠骨骼肌GLUT-4蛋白表達(dá)呈現(xiàn)先下降后上升的趨勢，其中G組（低氧運動后0.2MPa的增壓1h恢復(fù)）上漲幅度最大。 (4)通過7天增壓輔助方法干預(yù)后，G組（低氧運動后增壓恢復(fù)）與F組（低氧運動后自然恢復(fù)）相比大鼠骨骼肌LDH活性上升且兩組具有顯著性差異（P0.05） (5)通過7天增壓輔助方法干預(yù)后，大鼠骨骼肌SDH活性從A組（安靜對照）至G組（低氧運動后增壓恢復(fù)）呈現(xiàn)先上升后下降再上升再下降的趨勢且各組之間的差異不具有顯著性。結(jié)論： （1）增壓輔助方法干預(yù)的C組（0.2MPa增壓1h恢復(fù)）、D組（0.2MPa增壓2h恢復(fù)）、E組（0.3MPa增壓1h恢復(fù)）大鼠骨骼肌HIF-1α蛋白表達(dá)量上調(diào)，表明施加增壓輔助方法對大鼠骨骼肌HIF-1α產(chǎn)生影響，HIF-1α表達(dá)上升可誘導(dǎo)其下游的靶基因轉(zhuǎn)錄上調(diào)增加骨骼肌氧及營養(yǎng)物質(zhì)的供應(yīng)，有利于高原低氧環(huán)境下大強(qiáng)度訓(xùn)練后的疲勞恢復(fù)。 （2）增壓輔助干預(yù)后G組（低氧運動后0.2MPa的增壓1h恢復(fù)）與F組（低氧運動后自然恢復(fù)）相比GLUT-1、GLUT-4有明顯上升趨勢。GLUT-1、GLUT-4表達(dá)上升可增加骨骼肌的葡萄糖轉(zhuǎn)運能力，有利于骨骼肌對葡萄糖的攝取及能量物質(zhì)的補(bǔ)充，促進(jìn)機(jī)體在高原低氧環(huán)境下大強(qiáng)度訓(xùn)練后的疲勞恢復(fù)。 （3）增壓輔助干預(yù)后C組（0.2MPa增壓1h恢復(fù)），E組（0.3MPa增壓1h恢復(fù)）大鼠骨骼肌LDH的活性上調(diào)；G組（低氧運動后0.2MPa增壓1h恢復(fù)）與F組（低氧運動后自然恢復(fù)）相比具有顯著性（P0.05）。表明增壓輔助方法干預(yù)的恢復(fù)組LDH活性上調(diào)可促進(jìn)骨骼肌乳酸至丙酮酸的代謝，此過程可減少骨骼肌中乳酸的含量，增加對骨骼肌能量的供應(yīng)，有助于高原低氧環(huán)境運動后機(jī)體的疲勞恢復(fù)。 （4）增壓輔助干預(yù)后大鼠骨骼肌SDH活性無顯變化，表明高原低氧大強(qiáng)度運動后采用增壓輔助方法對提高大鼠有氧代謝能力的影響不大。
[Abstract]:Objective: to establish rat in high intensity exercise at high altitude, transferred to the pressurized cabin inner 0.2-0.3MPa (absolute pressure 2-3ATA) animal model of restoring pressure in the environment, explore the plateau environment of high intensity training and applied pressure assisted method for HIF-1 rat skeletal muscle alpha, GLUT-1, GLUT-4 the expression of LDH protein and enzyme activity, influence of SDH on the recovery of skeletal muscle fatigue in rats.
Methods: 56 healthy male Wistar rats were randomly divided into 7 groups, 8 rats in each group: group A was the control group, B group, C group natural recovery group, recovery group 0.2MPa 1H group D 0.2MPa turbocharged and supercharged 2H recovery group, E group for 0.3MPa turbocharged 1H recovery group F group, hypoxia after exercise the natural recovery group, G group after hypoxic exercise 0.2MPa turbocharged 1H recovery group; the average weight of group A was 197.71 + 16.87g, 210.25 + 12.06g B group, C group and D group was 208.38 + 4.75g, 208 + 14.33g, 210.88 + 11.99g E group, F group of 212.75 + 12.09g, 210.50 + 8.53g.7 group and G group rats in Xining (2260m) real animal experiments in Medical College of Qinghai University for 3 days and 6 days of formal training adaptation training: the adaptation period of first days of running 15m/min, then added every day 5m/min; adaptation period of slope is 0 degrees; the movement time of the first day of 15min, 20min in second days, third days 30min. formal training first days running 25m/min second. 30m/min day, after 4 days The end of the experiment to maintain the running speed; day 1,2 slope 0 degrees to 10 degrees for third days, the remaining 3 day to maintain this invariant gradient; the formal training exercise during the time is 60min/ days. Seventh days B group G and group velocity is 35m/mim, slope of 10 degrees for the exhaustive exercise. After exercise in exhaustive exercise groups the sequence in B group, F group into the cabin pressurization recovery 1H, booster recovery after 24h rats were anesthetized for the implementation of all side of the gastrocnemius muscle were detected by Western blotting of HIF-1 alpha, GLUT-1, expression of GLUT-4 protein, using ELISA method to detect the LDH and SDH activity:
(1) after 7 days of high altitude hypoxic environment, the HIF-1 C protein expression in the skeletal muscle of rats showed a trend of first decline, then increased and then decreased. The C group (0.2MPa booster 1H recovery) increased significantly.
(2) after 7 days of high altitude hypoxic environment, after boosting exercise, the GLUT-1 protein expression in skeletal muscle of rats showed a trend of first rise, then decline and then increased. Among them, G group (0.2MPa booster 1H recovery after hypoxia exercise) increased significantly.
(3) after 7 days of high-altitude hypoxia, the recovery of GLUT-4 protein expression in skeletal muscle of rats showed a trend of first decline and then increased. After that, G group (0.2MPa booster 1H recovery after hypoxia) increased the most.
(4) after 7 days of booster intervention, the activity of LDH in skeletal muscle of G group increased significantly compared with that in group F (after hypoxic exercise), and there was a significant difference between the two groups (P0.05).
(5) after 7 days of pressurization assisted intervention, the SDH activity of skeletal muscle from A group (quiet control) to G group (after hypoxic exercise supercharging recovery) increased at first, then decreased, then increased and then decreased.
(1) C group intervention (0.2MPa booster assisted method, turbocharged 1H recovery) D group (0.2MPa group, E turbocharged 2H recovery (0.3MPa) turbocharged 1H recovery) expression in rat skeletal muscle HIF-1 protein was up-regulated, showed that applying boost auxiliary method influence on HIF-1 alpha in rat skeletal muscle, the expression of HIF-1 a target up regulation of gene transcription induced increased downstream of the increase in skeletal muscle oxygen and nutrient supply, is conducive to the recovery of fatigue after high intensity training in hypoxia.
(2) after the intervention group G (auxiliary booster booster 1H 0.2MPa recovery after hypoxic exercise group (F) and natural recovery after hypoxic exercise) compared to GLUT-1, GLUT-4 has significantly increased.GLUT-1, increased GLUT-4 expression can increase glucose transport in skeletal muscle, which is beneficial to increase in skeletal muscle glucose uptake and energy the material, promote recovery of body fatigue in high intensity training in altitude hypoxia environment.
The auxiliary booster (3) after the intervention of C (0.2MPa group, E group, 1H booster recovery (0.3MPa) turbocharged 1H recovery) increases the activity of skeletal muscle of rats with LDH; group G (0.2MPa turbocharged 1H recovery after hypoxic exercise group (F) and natural recovery after hypoxic exercise) significantly compared (P0.05) shows that the increases. Pressure assisted method intervention recovery group upregulation of LDH activity can promote skeletal muscle lactate to pyruvate metabolism, this process can reduce the content of lactic acid in skeletal muscle, increase of muscle energy supply, helps the body fatigue recovery in the plateau hypoxia environment after exercise.
(4) there was no significant change in SDH activity in skeletal muscle of rats after booster assisted intervention, indicating that booster assisted method after high altitude hypoxia had little effect on improving aerobic metabolism ability in rats.

【學(xué)位授予單位】：青海師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：G804.7

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王榮輝,劉桂華,胡琪,張一民;低氧訓(xùn)練對大鼠骨骼肌乳酸脫氫酶和蘋果酸脫氫酶活性的影響[J];北京體育大學(xué)學(xué)報;1998年03期

2 黃緘,黃慶愿,高鈺琪,陳健,范有明,廖衛(wèi)公;缺氧習(xí)服大鼠骨骼肌葡萄糖轉(zhuǎn)運體特點的研究[J];第三軍醫(yī)大學(xué)學(xué)報;2004年18期

3 辛佩珠，藺世龍，湯炎，周扣珍，王慶蓉，，劉景昌;高壓氧對沙鼠腦缺血時海馬及微血管形態(tài)的影響[J];第一軍醫(yī)大學(xué)學(xué)報;1996年02期

4 郭俊華;;高壓氧治療學(xué)在臨床中的應(yīng)用[J];工企醫(yī)刊;2011年05期

5 王偉 ,張芳,哈振德,張西洲,馬勇,崔建華;富氧室在海拔3700m對人體做功效率的影響[J];高原醫(yī)學(xué)雜志;2003年01期

6 李裕和;運動性腦缺氧與中樞疲勞[J];廣州體育學(xué)院學(xué)報;1997年02期

7 劉金柱;;模擬梯度交替海拔低氧訓(xùn)練對大鼠骨骼肌相關(guān)代謝的影響[J];德州學(xué)院學(xué)報;2012年04期

8 常建軍;李莉;;有氧運動對小鼠心肌、骨骼肌線粒體SOD、MDA、LDH的影響[J];湖北農(nóng)業(yè)科學(xué);2009年08期

9 賈磊;聶秀娟;方梅;;實驗性高海拔對犬SDH、LDH活性與骨骼肌超微結(jié)構(gòu)的影響及其運動學(xué)意義[J];吉林體育學(xué)院學(xué)報;2010年01期

10 崔建華,張西洲,哈振德,王偉,朱永安,馬勇,張芳;富氧室對高原人體運動血乳酸、血氨及肌紅蛋白的影響[J];解放軍預(yù)防醫(yī)學(xué)雜志;2002年04期

本文編號：1546632