發(fā)布時(shí)間：2019-02-17 19:14

【摘要】： 血吸蟲病是我國十一五期間提出的四大重點(diǎn)控制疾病(艾滋病、結(jié)核病和乙肝)之一,是我國危害最為嚴(yán)重的寄生蟲病,自從2005年首次報(bào)道空心蓮子草浸提液含有抑制釘螺運(yùn)動(dòng)的化學(xué)成分后,我們(武漢科技大學(xué)和貴州大學(xué))發(fā)現(xiàn)用全草干粉的浸提液(1g/100ml水)可在短時(shí)間內(nèi)使釘螺失去運(yùn)動(dòng)能力,本課題繼續(xù)在前一階段的研究成果的基礎(chǔ)上,對空心蓮子草中所含有的有效化學(xué)成分進(jìn)行提取分離、純化。 空心蓮子草Alternanthera philoxeroides(Mart.)Griseb.,為莧科蓮子草屬植物,具有清熱、涼血、解毒等功效,用于治療麻疹、流行性感冒等病毒性疾患。本文的研究內(nèi)容和結(jié)果如下: 研究空心蓮子草抑制釘螺運(yùn)動(dòng)的機(jī)理:將實(shí)驗(yàn)釘螺隨機(jī)分為4組,分別于空心蓮子草浸液(1 g/L)及去氯水中各浸泡12 h和20 h后,用酶組織化學(xué)方法染色,顯微鏡下觀察釘螺的頭足部、中樞神經(jīng)節(jié)、鰓葉及肝臟的三磷酸腺苷酶(Mg~(2+)-ATPase)、膽堿脂酶(ChE)、乳酸脫氫酶(LDH)和琥珀酸脫氫酶(SDH)的活性;并用計(jì)算機(jī)圖像分析系統(tǒng)(HPIAS-1000)檢測各組不同部位染色片的灰度值,進(jìn)行定量分析。得出結(jié)論空心蓮子草浸液主要通過迅速抑制釘螺體內(nèi)ChE活性,隨后抑制Mg~(2+)-ATPase活性,而導(dǎo)致ATP的釋放與利用障礙致釘螺死亡。 對莧科蓮子草屬植物空心蓮子草的化學(xué)成分進(jìn)行研究:空心蓮子草干燥后粉碲,經(jīng)0.1mol/L HCl滲漉提取,石油醚萃取,用氨水調(diào)酸水層pH≈12,經(jīng)氯仿萃取,得氯仿層浸膏,浸膏反復(fù)經(jīng)硅膠柱色譜、葡聚糖凝膠(Sephadex LH-20)柱色譜、反相硅膠(RP-18)柱色譜分離純化得單一成分,結(jié)構(gòu)用現(xiàn)代波譜分析技術(shù)(MS、NMR、HPLC等)和理化方法鑒定。從該種植物中分離得到一個(gè)單一成分為生物堿,鑒定為β-carboline(APG-2)。得出結(jié)論為化合物APG-2為首次從該植物中分離得到。
[Abstract]:Schistosomiasis is one of the four major diseases (AIDS, tuberculosis and hepatitis B) put forward during the 11th Five-Year Plan in China. It is the most serious parasitic disease in China. Since 2005, when it was first reported that the extract of lotus seed contains chemical constituents that inhibit the movement of Oncomelania hupensis, We (Wuhan University of Science and Technology and Guizhou University) found that the extraction solution (1g/100ml water) of whole straw dry powder can make snail lose movement ability in a short time. The active chemical constituents of lotus seed were extracted and purified. Alternanthera philoxeroides (Mart.) Griseb., is a plant of the genus Lotus Seeds in Amaranaceae. It has the functions of clearing heat, cooling blood and detoxifying. It is used to treat viral diseases such as measles, influenza and so on. The research contents and results are as follows: to study the mechanism of inhibition of Oncomelania hupensis movement by lotus seed grass: the experimental snails were randomly divided into 4 groups. The snails were soaked in lotus seed extract (1 g / L) and dechlorinated water for 12 h and 20 h, respectively. The head, foot and central nervous ganglion of Oncomelania hupensis were observed under microscope by enzyme histochemical staining. Activities of Mg~ (2)-ATPase), cholinesterase (ChE), lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) in Gill leaves and liver; Computer image analysis system (HPIAS-1000) was used to detect the grayscale value of each group. Conclusion the results showed that the extract of lotus seeds could rapidly inhibit the activity of ChE in Oncomelania hupensis, and then inhibit the activity of Mg~ (2)-ATPase, which resulted in the release and utilization of ATP and resulted in the death of Oncomelania hupensis. In this paper, the chemical constituents of Hollow Lotus Seeds of Amaranaceae were studied. After drying, the powder and tellurium were extracted by 0.1mol/L HCl percolation, petroleum ether was extracted, and pH 鈮，

本文編號(hào)：2425505