【摘要】：目的:研究解脲支原體(Ureaplasma urealyticum,Uu)在健康晚孕期孕婦以及健康新生兒泌尿生殖道中的檢出情況與分群分型,以探討Uu在該人群泌尿生殖道中的流行病學(xué)情況。 方法:收集健康晚孕期孕婦宮頸口分泌物85例,足月分娩的健康新生兒外陰分泌物124例,以上標(biāo)本包括母嬰37對(duì)。收集到的分泌物直接接種于解脲支原體液體選擇性培養(yǎng)基進(jìn)行初篩,初篩陽(yáng)性的標(biāo)本用PCR法進(jìn)行分群。對(duì)Uu一群的標(biāo)本再用PCR法及酶切法進(jìn)一步分型。采用SPSS 10.0統(tǒng)計(jì)軟件包進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果:85例健康晚孕期孕婦中Uu陽(yáng)性51例(60.00%),其中單純生物一群40例(78.43%),單純生物二群7例(13.73%),兩群混合4例(7.84%)。生物一群中1型11例(27.50%),6型10例(25.00%),3/14型14例(35.00%),混合型5例(12.50%)。124例新生兒中Uu陽(yáng)性13例(10.48%),其中,經(jīng)陰娩58例中陽(yáng)性12例(20.69%),剖宮產(chǎn)66例中陽(yáng)性1例(1.52%),兩組陽(yáng)性率差別有統(tǒng)計(jì)學(xué)意義(x~2=12.09,P0.01)。77例男嬰中陽(yáng)性4例(5.19%),47例女?huà)胫嘘?yáng)性9例(19.15%),兩組陽(yáng)性率差別有統(tǒng)計(jì)學(xué)意義(x~2=6.055,P0.05)。13例Uu陽(yáng)性的新生兒標(biāo)本中,生物一群9例(69.23%),生物二群3例(23.08%),混合群1例(7.6996)。生物一群中1型2例,6型5例,3/14型1例,混合型1例。37對(duì)母嬰中,母親Uu陽(yáng)性20例(其中經(jīng)陰娩陽(yáng)性14例,剖宮產(chǎn)陽(yáng)性6例),嬰兒陽(yáng)性6例,均為經(jīng)陰分娩嬰兒,新生兒的Uu分型結(jié)果與其母親Uu分型結(jié)果相一致。 結(jié)論:解脲支原體在健康晚孕期孕婦及足月分娩的健康新生兒泌尿生殖道中的攜帶率分別為60.00%與10.48%,經(jīng)陰分娩組新生兒Uu的檢出率高于剖宮產(chǎn)組,女?huà)胪怅嶶u檢出率高于男嬰,各人群組分離的Uu以生物一群為主;新生兒泌尿生殖道Uu分型結(jié)果與其母親相一致,母親的產(chǎn)道可能是新生兒泌尿生殖道Uu存在的最初來(lái)源。Uu可能屬于人體的一種共生微生物。
[Abstract]:Objective: to study the detection and classification of Ureaplasma Urealyticum (Ureaplasma urealyticum,Uu) in the genitourinary tract of pregnant women and newborns during late pregnancy and to explore the epidemiology of Uu in urogenital tract. Methods: 85 cases of cervical secretion from pregnant women and 124 cases of vulva secretions from term delivery were collected, including 37 pairs of mothers and infants. The collected secretions were inoculated directly into Ureaplasma Urealyticum liquid selective medium for primary screening. The positive samples were divided into groups by PCR method. A group of Uu specimens were further typed by PCR and enzyme digestion. SPSS 10.0 statistical software package was used for statistical analysis. Results among 85 healthy pregnant women with late pregnancy, 51 (60.00%) were positive for Uu, including 40 (78.43%) in the first group, 7 (13.73%) in the second group, and 4 (7.84%) in the mixed group. There were 11 cases (27.50%) of type 1 in biological group, 10 cases (25.00%) of type 6, 14 cases of type 3 / 14 (35.00%), and 13 cases (10.48%) of positive Uu in 5 cases (12.50%) of mixed type. Of 58 cases of vaginal delivery, 12 cases (20.69%) were positive, and 66 cases of cesarean section were positive in 1 case (1.52%). There was a significant difference between the two groups (xan212.09 / P0.01) .77 male infants were positive in 4 cases (5.19%) and 47 cases in female infants (9 cases (19.15%). There was a significant difference between the two groups (XJ 26.055, P0.05). 13 cases of Uu. Positive neonatal specimens, There were 9 cases (69.23%) in the first group, 3 cases (23.08%) in the second group and 1 case (7.6996) in the mixed group. In one group of biology, 2 cases of type 1, 5 cases of type 6, 1 case of type 3 / 14, and 1 case of mixed type. 37 pairs of mother and infant, 20 cases of mother Uu positive (14 cases of transvaginal delivery, 6 cases of cesarean section), 6 cases of positive infant, all of them were transvaginal delivery. The results of Uu typing of newborns were consistent with the results of Uu typing of their mothers. Conclusion: the carrying rates of Ureaplasma Urealyticum in the genitourinary tract of healthy late pregnant women and full-term newborns were 60.00% and 10.48, respectively. The detection rate of Uu was higher in the transvaginal delivery group than in the cesarean section group, and the detection rate of vulvar Uu in female infants was higher than that in male infants. The Uu isolated from each group was mainly biotic, and the Uu typing results of neonate genitourinary tract were consistent with those of mother. The mother's birth canal might be the original source of neonatal urogenital tract Uu. Uu might belong to a symbiotic microorganism of human body.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類(lèi)號(hào)】：R181.3

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