【摘要】： 1杭州市區(qū)人群主觀乳糖不耐受流行病學(xué)調(diào)查 1.1杭州市區(qū)人群乳糖不耐受發(fā)生率調(diào)查及相關(guān)因素分析 背景:由于乳糖酶的缺乏,很多人群攝入含一定量乳糖的牛奶后出現(xiàn)腹部不適癥狀,主觀認(rèn)為是乳糖不耐受患者,因此避免食用乳制品,這可能會(huì)給他們帶來長(zhǎng)期的健康危害。而目前卻缺乏有關(guān)人群主觀乳糖不耐受的報(bào)道以及由此引起的牛奶及奶制品攝入的變化。 目的:了解杭州市區(qū)人群中主觀乳糖不耐受的發(fā)生率,并對(duì)其相關(guān)因素進(jìn)行探討。 方法:按照分層整群隨機(jī)抽樣調(diào)查原則,在杭州市抽取3個(gè)區(qū),然后在每個(gè)區(qū)中各抽取一個(gè)社區(qū)作為調(diào)查點(diǎn),對(duì)社區(qū)居民進(jìn)行抽樣調(diào)查。采用問卷調(diào)查的方式,以面對(duì)面詢問的方式回答問卷。問卷內(nèi)容包括個(gè)人飲用牛奶及奶制品情況(包括種類及量),飲用牛奶及奶制品后是否出現(xiàn)腹部不適癥狀(包括腹脹、胃腸脹氣、腹鳴、腹痛、腹瀉、惡心、嘔吐等),以及生活事件量表、醫(yī)院焦慮抑郁量表和腸道癥狀量表。采用SPSS 13.0統(tǒng)計(jì)軟件來進(jìn)行數(shù)據(jù)的統(tǒng)計(jì)分析,Mann-Whitney U test方法用于非正態(tài)分布的兩組之間的比較,Logistic回歸分析用于評(píng)價(jià)乳糖攝入在性別、年齡段之間有無差異以及主觀乳糖不耐受者與乳糖耐受者之間的多因素分析。 結(jié)果:本研究共調(diào)查了800人,有68人因調(diào)查問卷資料填寫不完整而剔除出本研究,最后納入分析的有效調(diào)查問卷有732份,男性261例,女性471例,年齡17-75歲,平均46.12±14.77歲。主觀乳糖不耐受者135例,男性40例,女性95例,平均年齡45.59±14.60歲,其中輕度腹部不適111例,中度不適16例,重度不適8例。人群中以牛奶消費(fèi)為主,其次是酸奶及調(diào)味奶,而奶粉及煉乳或濃縮乳消費(fèi)者很少,大多數(shù)消費(fèi)酸奶量多的人群卻對(duì)牛奶的消費(fèi)量相對(duì)少。人群乳制品消費(fèi)種類不同的原因中,無飲用乳制品習(xí)慣的有413例(56.4%,413/732),有166例認(rèn)為不喜歡某種(些)乳制品的口味(22.7%),有135例認(rèn)為自己飲用乳制品后出現(xiàn)腹部癥狀(18.4%),只有18例(2.5%)認(rèn)為經(jīng)濟(jì)上難以承受對(duì)于乳制品的每日消費(fèi)。乳制品中乳糖的攝入量,女性高于男性,差異有統(tǒng)計(jì)學(xué)意義,且隨著年齡的增長(zhǎng),乳糖攝入量逐漸減少,30歲以下人群和60歲以上人群間乳糖攝入存在顯著性差異。主觀乳糖不耐受者乳制品及乳糖的攝入量較無癥狀的人群明顯減少,差異有統(tǒng)計(jì)學(xué)意義。主觀乳糖不耐受者與耐受者在性別、年齡、家庭人均月收入、職業(yè)及受教育程度等方面無顯著性差異。在焦慮評(píng)分、抑郁評(píng)分、生活應(yīng)激評(píng)分及腸道癥狀評(píng)分方面,主觀乳糖不耐受者均高于耐受者,但僅焦慮評(píng)分的差異有統(tǒng)計(jì)學(xué)意義。 結(jié)論:杭州市區(qū)人群隨著年齡的增長(zhǎng),乳制品及乳糖攝入量逐漸減少。人群中主觀乳糖不耐受的比率較高,且主觀乳糖不耐受者乳制品及乳糖的攝入量較無癥狀人群明顯減少。焦慮狀態(tài)水平在主觀乳糖不耐受者中高于乳糖耐受者。 1.2主觀乳糖不耐受和實(shí)際乳糖不耐受的相關(guān)性的研究 背景:關(guān)于乳糖不耐受的研究很多,然而對(duì)于乳糖不耐受的誤解仍然比較普遍。主觀乳糖不耐受人群中部分實(shí)際上能正常消化乳糖,因此對(duì)主觀乳糖不耐受人群的判定很重要。 目的:探討主觀乳糖不耐受與實(shí)際乳糖不耐受的情況,評(píng)價(jià)主觀乳糖不耐受與實(shí)際乳糖不耐受的相關(guān)性。 方法:研究對(duì)象為在杭州居住的人群,根據(jù)納入和排除標(biāo)準(zhǔn)最終確定入選的研究對(duì)象。對(duì)研究對(duì)象采用問卷調(diào)查的方式,以面對(duì)面詢問的方式回答問卷。問卷內(nèi)容包括個(gè)人飲用牛奶及奶制品情況(包括種類及量),飲用牛奶及奶制品后是否出現(xiàn)腹部不適癥狀(包括腹脹、排氣增多、腹鳴、腹痛、腹瀉、惡心、嘔吐等),以及最近三個(gè)月內(nèi)腹痛及大便情況,和生活事件量表、醫(yī)院焦慮抑郁量表和腸道癥狀量表。個(gè)體飲用牛奶及奶制品后出現(xiàn)腹部不適癥狀或因上述腹部癥狀而拒絕飲用者,判定為主觀乳糖不耐受。所有研究對(duì)象均行乳糖氫呼氣試驗(yàn)檢查。若氫氣濃度升高超過基礎(chǔ)值20ppm以上水平并伴有消化道癥狀或出現(xiàn)消化道癥狀而不論氫氣濃度變化是否超過基礎(chǔ)值20ppm,判定為乳糖不耐受;氫氣濃度升高超過基礎(chǔ)值20ppm以上水平但不伴有消化道癥狀為乳糖消化不良;氫氣濃度變化不超過基礎(chǔ)值20ppm水平,且不伴有消化道癥狀為乳糖正常消化。記錄氫呼氣試驗(yàn)檢查中和檢查后12小時(shí)內(nèi)的腹部癥狀評(píng)分,癥狀評(píng)分為攝入乳糖后12h內(nèi)各癥狀評(píng)分系數(shù)與其嚴(yán)重程度乘積之和。采用SPSS 13.0統(tǒng)計(jì)軟件來進(jìn)行統(tǒng)計(jì)分析�？ǚ綑z驗(yàn)用于主觀乳糖不耐受與氫呼氣試驗(yàn)檢測(cè)的實(shí)際乳糖不耐受之間的比較,Logistic回歸分析用于評(píng)價(jià)主觀乳糖不耐受者中三種乳糖消化吸收類型之間的多因素分析。單因素方差分析用于評(píng)價(jià)乳糖攝入在性別之間有無差異,Mann-Whitney U test方法用于非正態(tài)分布的兩組之間的比較。 結(jié)果:共有209人納入本研究,且都經(jīng)歷了20g乳糖氫呼氣試驗(yàn)檢查。209例中,乳糖不耐受者139例(66.5%),男性74例,女性65例;乳糖消化不良37例(17.7%),男性17例,女性20例;乳糖正常消化者33例(15.8%),男性14例,女性19例。三種類型在男女性別方面無統(tǒng)計(jì)學(xué)差異。本研究中主觀乳糖不耐受54例,占本研究對(duì)象的25.8%(54/209),其中經(jīng)乳糖氫呼氣試驗(yàn)診斷為乳糖不耐受42例(77.8%,42/54),乳糖消化不良5例(9.2%),乳糖正常消化7例(13.0%)。問卷調(diào)查的主觀乳糖不耐受對(duì)于實(shí)際乳糖不耐受判定的敏感度為30.2%(42/139),特異度為82.9%(58/70),陽性預(yù)測(cè)值為77.8%(42/54),陰性預(yù)測(cè)值為37.4%(58/155)。54例主觀乳糖不耐受者采用ROMEⅢ標(biāo)準(zhǔn)來診斷,有35例為腸易激綜合征,其中實(shí)際乳糖不耐受29例,乳糖消化不良3例,乳糖正常消化3例。主觀乳糖不耐受中的實(shí)際乳糖不耐受、乳糖消化不良及乳糖正常吸收與腸易激綜合征之間無統(tǒng)計(jì)學(xué)差異(p=0.394)。主觀乳糖不耐受者中三種乳糖消化吸收的類型之間在性別、年齡、家庭人均月收入、職業(yè)及受教育程度、焦慮評(píng)分、抑郁評(píng)分、生活應(yīng)激評(píng)分及腸道癥狀評(píng)分等方面均無統(tǒng)計(jì)學(xué)差異。 結(jié)論:主觀乳糖不耐受不能準(zhǔn)確地反應(yīng)中國人群中實(shí)際乳糖不耐受情況。主觀乳糖不耐受的靈敏度低且特異度不夠高,不能很好地判定實(shí)際乳糖不耐受,但其有一定的陽性預(yù)測(cè)值,有助于提高實(shí)際乳糖不耐受的檢出率。對(duì)于主觀乳糖不耐受者,應(yīng)行乳糖氫呼氣試驗(yàn)以明確其具體類型并排除被誤認(rèn)為是主觀乳糖不耐受的人群。另外,乳糖氫呼氣試驗(yàn)?zāi)馨l(fā)現(xiàn)一些潛在的乳糖不耐受者。 2乳糖不耐受與乳糖消化不良間差異的機(jī)制研究 背景:在乳糖酶缺乏者中,一些人群表現(xiàn)為乳糖消化不良,而另一些人群則表現(xiàn)為乳糖不耐受,且不耐受癥狀各異,對(duì)于乳糖不耐受與乳糖消化不良間差異的機(jī)制目前尚未十分明確。 目的:探討乳糖不耐受與乳糖消化不良在胃腸道動(dòng)力及腸道菌群間的差異,以及它們之間的相互影響。 方法:對(duì)無器質(zhì)性疾病的成年人,行乳糖氫呼氣試驗(yàn)檢查及記錄腹部癥狀(腹鳴、腹脹、腹痛及腹瀉等),確定其是否為乳糖不耐受、乳糖消化不良或乳糖正常消化。評(píng)估乳糖不耐受者的腹部癥狀評(píng)分,并通過氫呼氣試驗(yàn)評(píng)價(jià)口盲腸轉(zhuǎn)運(yùn)時(shí)間。運(yùn)用熒光原位雜交技術(shù),選擇5種特異性的探針檢測(cè)乳糖不耐受、乳糖消化不良及乳糖正常消化者的腸道菌群情況。 結(jié)果:163例研究對(duì)象中,男性80例,女性83例,其中乳糖不耐受有117例(男性62例,女性55例),乳糖消化不良24例(男性9例,女性15例),乳糖正常消化22例(男性9例,女性13例)。乳糖不耐受與乳糖消化不良在年齡及性別之間無統(tǒng)計(jì)學(xué)差異。乳糖不耐受的口盲腸轉(zhuǎn)運(yùn)時(shí)間為54.23±30.12分鐘,而乳糖消化不良的口盲腸轉(zhuǎn)運(yùn)時(shí)間為77.50±31.49分鐘,兩者間差異有統(tǒng)計(jì)學(xué)意義,且口盲腸轉(zhuǎn)運(yùn)時(shí)間的OR值為0.977,有統(tǒng)計(jì)學(xué)意義(p=0.003)。運(yùn)用熒光原位雜交技術(shù),發(fā)現(xiàn)腸道總細(xì)菌量及4種特異性探針?biāo)鶎?duì)應(yīng)的腸道細(xì)菌量在乳糖不耐受、乳糖消化不良及乳糖正常消化三者間存在差異。與Bac303探針雜交的擬桿菌屬和普霍沃勒菌屬及與Erec482探針雜交的直腸真桿菌-球形梭菌屬,在乳糖不耐受者中明顯高于乳糖消化不良和乳糖正常消化者;而與Bif164雜交的雙歧桿菌屬及與Lab722雜交的乳桿菌屬,在乳糖不耐受者中明顯低于乳糖消化不良和乳糖正常消化者,p值均0.05,差異有統(tǒng)計(jì)學(xué)意義。 結(jié)論:口盲腸轉(zhuǎn)運(yùn)時(shí)間和腸道菌群組成變化與乳糖不耐受密切相關(guān)。乳糖不耐受者的口盲腸轉(zhuǎn)運(yùn)時(shí)間較乳糖消化不良者明顯縮短。乳糖不耐受、乳糖消化不良及乳糖正常消化三者間的腸道菌群組成存在顯著差異。乳糖不耐受者的腸道擬桿菌屬和普霍沃勒菌屬及直腸真桿菌-球形梭菌屬明顯高于乳糖消化不良和乳糖正常消化者,而雙歧桿菌屬及乳桿菌屬明顯低于乳糖消化不良和乳糖正常消化者。 3杭州市區(qū)人群乳糖酶基因多態(tài)性與乳糖酶持續(xù)的相關(guān)性的研究 背景:乳糖酶持續(xù)的頻率在不同人群中存在很大的差異,乳糖酶基因的T-13910位點(diǎn)在歐洲人群中與乳糖酶持續(xù)密切相關(guān),然而其在非洲人群和中國北部人群并無明顯的相關(guān)性。 目的:探討杭州市區(qū)人群中乳糖酶基因T-13910等位基因位點(diǎn)情況,以及鄰近基因的單核苷酸多態(tài)性與乳糖酶持續(xù)的相關(guān)性。 方法:對(duì)無器質(zhì)性疾病的杭州市區(qū)成年人,行乳糖氫呼氣試驗(yàn)檢查,確定其是否為乳糖酶持續(xù)或乳糖酶不持續(xù),并抽取外周血行全血DNA提取,進(jìn)行PCR擴(kuò)增及測(cè)序,將DNA測(cè)序結(jié)果進(jìn)行比對(duì)分析以明確T-13910等位基因位點(diǎn)情況,并發(fā)現(xiàn)有無新的基因多態(tài)性位點(diǎn)。然后將乳糖酶基因型與乳糖酶表現(xiàn)型進(jìn)行比較及相關(guān)性分析。 結(jié)果:有163例納入研究,其中男性80例,女性83例,年齡21-70歲,平均年齡42.20±11.45歲。經(jīng)乳糖氫呼氣試驗(yàn)確定為乳糖酶持續(xù)型22例(13.5%,22/163),男性9例,女性13例;另141例為乳糖酶不持續(xù)型(86.5%,141/163),男性71例,女性70例。在141例乳糖酶不持續(xù)型中,乳糖不耐受117例(83.0%),乳糖消化不良24例(17.0%)。乳糖酶持續(xù)與乳糖酶不持續(xù)在男女性別方面無統(tǒng)計(jì)學(xué)差異。在所有被研究人群中均未檢測(cè)到T-13910基因多態(tài)性位點(diǎn),均為純合子C/C-13910。也發(fā)現(xiàn)人群中存在多個(gè)不同于C/T-13910的新的單核苷酸多態(tài)性位點(diǎn),它們分別為C/T-13908、A/C-13926、G/A-14010、T/G-13951、C/T-13952,其中A/C-13926出現(xiàn)在3例不同的DNA序列中,在2例不同的DNA序列中發(fā)現(xiàn)C/T-13908多態(tài)性位點(diǎn),G/A-14010單核苷酸多態(tài)性位點(diǎn)存在于1例中,而T/G-13951及C/T-13952多態(tài)性位點(diǎn)存在于同一例DNA序列中。T-13908等位基因均存在于乳糖酶持續(xù)中;C-13926等位基因均存在于乳糖酶不持續(xù)中,且T-13908等位基因與乳糖酶持續(xù)相關(guān),有統(tǒng)計(jì)學(xué)意義(p=0.017)。而C-13926等位基因與乳糖酶不持續(xù)的相關(guān)性無統(tǒng)計(jì)學(xué)意義。另外,A-14010、G-13951、T-13952等位基因多態(tài)性位點(diǎn)與乳糖酶表現(xiàn)型的相關(guān)性亦無統(tǒng)計(jì)學(xué)意義。 結(jié)論:首次發(fā)現(xiàn)乳糖酶基因在杭州市區(qū)人群中有多個(gè)新的單核苷酸多態(tài)性位點(diǎn),包括T-13908、C-13926、A-14010、G-13951、T-13952等位基因。T-13908等位基因可能與杭州市區(qū)人群的乳糖酶持續(xù)相關(guān)。乳糖酶持續(xù)在杭州市區(qū)人群比較少見,乳糖酶基因上游的T-13910等位基因位點(diǎn)的頻率很低。T-13910等位基因位點(diǎn)檢測(cè)法在杭州市區(qū)人群中不能預(yù)測(cè)乳糖酶持續(xù)。
[Abstract]:Epidemiological survey of Subjective Lactose Intolerance in 1 urban population of Hangzhou
Prevalence of lactose intolerance and related factors in 1.1 urban population of Hangzhou
BACKGROUND: Due to the lack of lactase, many people suffer from abdominal discomfort after intake of milk containing a certain amount of lactose. They are subjectively considered to be patients with lactose intolerance. Therefore, avoidance of dairy products may cause long-term health hazards to them. However, there are no reports on Subjective Lactose Intolerance in the population and its causes. Changes in intake of milk and dairy products.
Objective: To investigate the incidence of Subjective Lactose Intolerance in Hangzhou urban population and to explore the related factors.
Methods: According to the principle of stratified cluster random sampling survey, three districts were selected in Hangzhou, and then one community in each district was selected as the survey point to conduct a sampling survey of community residents. Types and doses, abdominal discomfort symptoms (including abdominal distention, gastrointestinal distention, ringing, abdominal pain, diarrhea, nausea, vomiting, etc.) and life events scale, Hospital Anxiety and depression scale and intestinal symptom scale. SPSS 13.0 statistical software was used for statistical analysis of the data. Mann-Whitney U test method was used for statistical analysis. Logistic regression analysis was used to assess the differences in lactose intake between the two groups with non-normal distribution, gender, age, and multivariate analysis of Subjective Lactose Intolerance and lactose tolerance.
Results: A total of 800 subjects were investigated, 68 of them were excluded from the study because of incomplete questionnaire data. At last, 732 valid questionnaires were included in the analysis, including 261 males and 471 females, aged 17-75, with an average age of 46.12 + 14.77 years. There were 111 cases of moderate and mild abdominal discomfort, 16 cases of moderate discomfort and 8 cases of severe discomfort. Milk consumption was the main factor, followed by yoghurt and seasoned milk, while milk powder and condensed milk or concentrated milk consumers were few. Most of the people who consumed more yoghurt consumed less milk. There were 413 (56.4%, 413/732) dairy habits, 166 (22.7%) who thought they did not like the taste of certain (some) dairy products, 135 (18.4%) who thought they had abdominal symptoms after drinking dairy products, and only 18 (2.5%) who thought they could not afford the daily consumption of dairy products financially. The intake of dairy products and lactose in subjects with subjective lactose intolerance was significantly lower than that in asymptomatic subjects, and the difference was statistically significant. There was no significant difference in anxiety score, depression score, life stress score and intestinal symptoms score, but the difference was statistically significant.
Conclusion: The intake of dairy products and lactose decreases with age in Hangzhou urban population. The rate of Subjective Lactose intolerance is higher, and the intake of dairy products and lactose in Subjective Lactose Intolerance group is significantly lower than that in asymptomatic group. The level of anxiety in Subjective Lactose Intolerance group is higher than that in lactose intolerance group.
1.2 study on the correlation between Subjective Lactose Intolerance and actual lactose intolerance
BACKGROUND: There are many studies on lactose intolerance, but misunderstandings about lactose intolerance are still widespread. Some of the subjects with subjective lactose intolerance can actually digest lactose normally, so it is important to judge the subjects with Subjective Lactose intolerance.
Objective: To investigate the relationship between Subjective Lactose Intolerance and actual lactose intolerance.
Methods: The subjects were selected according to the inclusion and exclusion criteria of the people living in Hangzhou. The subjects were interviewed face-to-face to answer the questionnaires. The questionnaires included personal drinking of milk and dairy products (including types and quantities), and whether or not they drank milk and dairy products after drinking. Symptoms of abdominal discomfort (including abdominal distention, increased exhaust, abdominal tinnitus, abdominal pain, diarrhea, nausea, vomiting, etc.) and abdominal pain and feces in the last three months, life events scale, Hospital Anxiety and depression scale and intestinal symptom scale. Individuals who drink milk and dairy products develop abdominal discomfort or refuse to drink because of the above abdominal symptoms Lactose intolerance was defined as subjective lactose intolerance. All subjects underwent lactose-hydrogen breath test. If hydrogen concentration increased above the baseline level of 20 ppm and accompanied by gastrointestinal symptoms or gastrointestinal symptoms, whether or not the change in hydrogen concentration exceeded the baseline value of 20 ppm, it was defined as lactose intolerance; if hydrogen concentration increased beyond the baseline level of 20 ppm, lactose intole Lactose dyspepsia was found at baseline level of 20 ppm or above but without digestive tract symptoms; hydrogen concentration did not exceed baseline level of 20 ppm, and normal digestion of lactose was not found in patients with digestive tract symptoms. SPSS 13.0 statistical software was used for statistical analysis. Chi-square test was used for comparing Subjective Lactose intolerance with actual lactose intolerance detected by hydrogen breath test. Logistic regression analysis was used to evaluate the multivariate analysis of three types of lactose digestion and absorption in Subjective Lactose intolerance. Univariate analysis of variance was used to assess gender differences in lactose intake, and Mann-Whitney U test was used to compare two groups with non-normal distribution.
Results: A total of 209 subjects were enrolled in the study and all underwent 20g lactose hydrogen breath test. Among 209 subjects, 139 (66.5%) were lactose intolerant, 74 males and 65 females, 37 (17.7%) were lactose dyspepsia, 17 males and 20 females, 33 (15.8%) were lactose normal digestion, 14 males and 19 females. There were 54 cases of Subjective Lactose intolerance, accounting for 25.8% (54/209). 42 cases (77.8%, 42/54), 5 cases (9.2%) of lactose dyspepsia and 7 cases (13.0%) of lactose intolerance were diagnosed by lactose hydrogen breath test. The ROME III criteria were used to diagnose 54 cases of Subjective Lactose intolerance, 35 cases of irritable bowel syndrome, including 29 cases of actual lactose intolerance, 3 cases of lactose dyspepsia and 3 cases of normal lactose digestion. There was no significant difference between actual lactose intolerance, lactose dyspepsia, normal lactose uptake and irritable bowel syndrome (p = 0.394). There were three types of lactose digestion and absorption among subjects with Subjective Lactose intolerance, including sex, age, family monthly income, occupation and education, anxiety score, depression score, life stress score and intestine. There was no significant difference in symptom scores.
CONCLUSION: Subjective lactose intolerance can not accurately reflect the actual lactose intolerance in Chinese population. Subjective lactose intolerance has low sensitivity and specificity. It can not be used to judge actual lactose intolerance well, but it has a certain positive predictive value, which is helpful to improve the detection rate of actual lactose intolerance. Lactose hydrogen breath test should be performed to identify the specific type and exclude those mistaken for Subjective Lactose intolerance. In addition, Lactose hydrogen breath test can identify some potential lactose intolerants.
2 the mechanism of lactose intolerance and lactose dyspepsia
BACKGROUND: In lactase deficiency patients, some people show lactose intolerance, while others show lactose intolerance with different symptoms. The mechanism of the difference between lactose intolerance and lactose dyspepsia is still unclear.
Objective: To investigate the differences between lactose intolerance and lactose dyspepsia in gastrointestinal motility and intestinal flora, and their interaction.
Methods: Lactose-hydrogen breath test was used to examine and record the abdominal symptoms (ringing, distention, abdominal pain, diarrhea, etc.) in adults without organic diseases to determine whether they were lactose intolerance, lactose dyspepsia or normal lactose digestion. Five specific probes were selected to detect intestinal flora in lactose intolerance, lactose dyspepsia and normal lactose digestion by fluorescence in situ hybridization.
Results: Of the 163 subjects, 80 were males and 83 were females, 117 were lactose intolerance (62 males and 55 females), 24 were lactose dyspepsia (9 males and 15 females), and 22 were lactose normal digestion (9 males and 13 females). There was no significant difference in age and sex between lactose intolerance and lactose dyspepsia. The oral-cecum transit time was 54.23 (+ 30.12) minutes, while the oral-cecum transit time of lactose dyspepsia was 77.50 (+ 31.49) minutes. The OR value of the oral-cecum transit time was 0.977, which was statistically significant (p = 0.003). The total bacterial count in the intestine and the four specific probes were detected by fluorescence in situ hybridization (FISH). Bacterioids hybridized with Bac303 probe, Prohovolera and Clostridium sphaeroides hybridized with Erec482 probe were significantly higher in lactose intolerance than in lactose intolerance and lactose normal digestion. However, Bifidobacterium hybridized with Bif164 and Lactobacillus hybridized with Lab722 were significantly lower in lactose intolerant patients than those with lactose dyspepsia and normal lactose digestion, P values were 0.05, the difference was statistically significant.
CONCLUSION: The oral-cecum transit time and intestinal microflora composition are closely related to lactose intolerance. The oral-cecum transit time of lactose intolerant patients is significantly shorter than that of lactose intolerant patients. There are significant differences in intestinal microflora composition among lactose intolerance, lactose dyspepsia and normal lactose digestion. Bacillus, Prokhovella and Clostridium sphaeroides were significantly higher than those of lactose dyspepsia and lactose normal digestion, while Bifidobacterium and Lactobacillus were significantly lower than those of lactose dyspepsia and lactose normal digestion.
Correlation between lactase gene polymorphism and lactase persistence in 3 urban population of Hangzhou
BACKGROUND: The frequency of lactase persistence varies greatly among different populations. The T-13910 locus of the lactase gene is closely related to the persistence of lactase in European populations, but not in African populations and Northern China.
Objective: To investigate the allele T-13910 of lactase gene in Hangzhou urban population and the correlation between the single nucleotide polymorphism of adjacent genes and the persistence of lactase.
Methods: Adults without organic diseases in Hangzhou were examined by lactose hydrogen breath test to determine whether they were lactase persistent or lactase non-persistent. Peripheral blood was extracted for whole blood DNA extraction, PCR amplification and sequencing. The results of DNA sequencing were compared and analyzed to determine the T-13910 allele.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2009
【分類號(hào)】：R57;R181.3

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 楊建鋒;乳糖攝入在腹瀉型腸易激綜合征發(fā)病中的作用及機(jī)制研究[D];浙江大學(xué);2011年

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本文編號(hào)：2207800