本文選題：病毒性腦炎 + 病毒分離　； 參考：《山東大學(xué)》2005年碩士論文

【摘要】：目的:本研究是根據(jù)我省歷年來上報的乙型腦炎資料分析我省乙型腦炎的流行趨勢,并在不同生態(tài)環(huán)境中捕捉蚊蟲標(biāo)本,找出我省不同生態(tài)環(huán)境中的優(yōu)勢蚊種,同時用細(xì)胞培養(yǎng)的方法從蚊體內(nèi)分離病毒,并用常規(guī)PCR和熒光PCR的方法進(jìn)行初步鑒定,篩選乙型腦炎病毒,為以后對病毒進(jìn)行核苷酸序列分析、鑒定病毒種類、研究我省存在的乙腦病毒與國內(nèi)、外其他地域同種病毒的差異,繪制系統(tǒng)發(fā)生樹,進(jìn)而研究病毒的進(jìn)化規(guī)律和分析目前使用的疫苗有無保護(hù)作用打下基礎(chǔ)。 方法: 1.資料收集:根據(jù)歷年來我省上報的乙型腦炎資料進(jìn)行統(tǒng)計,參考我省已發(fā)表的有關(guān)此課題的文獻(xiàn),進(jìn)行分析乙腦流行趨勢及其原因。 2.標(biāo)本的收集運輸:在不同生態(tài)環(huán)境中將捕到蚊子,快速凍死后剔除雄蚊,將蚊子分類,50～100只為一組,裝入凍存管中,用醫(yī)用膠布封口,并用記號筆編號,凍存管放入布袋中后及時放入液氮中,送至實驗室。 3.根據(jù)在我省不同生態(tài)環(huán)境中采集蚊蟲標(biāo)本結(jié)果,分析我省不同生態(tài)環(huán)境
[Abstract]:Objective: to analyze the epidemic trend of Japanese encephalitis in our province according to the data of Japanese encephalitis reported over the years, and to capture mosquito specimens in different ecological environment and find the dominant species in different ecological environment in our province, and divide the virus from the mosquito in the body with the method of cell culture, and use the conventional PCR and fluorescence PCR methods. Preliminary identification, screening of Japanese encephalitis virus, in order to analyze the nucleotide sequence of the virus and identify the species of the virus in the future, and study the difference between the virus of the encephalitis B and the domestic and other regions of the same virus, draw the phylogenetic tree, and then study the evolution of the virus and analyze the protection of the current vaccine.
Method:
1. data collection: according to the statistics of Japanese encephalitis data reported in our province over the years, and referring to the published literature on this subject in our province, the trend of epidemic encephalitis B and its reasons are analyzed.
The collection and transportation of 2. specimens: the mosquitoes will be caught in different ecological environment, the mosquitoes are removed after quick freezing and the mosquitoes are removed. The mosquitoes are classified, 50~100 are one group, they are loaded into the cryopreservation tube, sealed with the medical glue cloth, and numbered with the marker pen. The cryopreservation pipe is put into the liquid nitrogen and sent to the laboratory in time after the storage tube is put into the cloth bag.
3. according to the results of collecting mosquito specimens in different ecological environment of our province, we analyze the different ecological environment in our province.
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R181.3

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前2條

1 楊猛;河南省乙型腦炎病毒的分離鑒定及分子特性分析[D];河南農(nóng)業(yè)大學(xué);2011年

2 魯蘇娜;淄博、洛陽兩地乙型腦炎病毒檢測及病毒性腦炎其它病原篩查[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2012年

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本文編號：2053416