本文選題：病毒性腦炎 + 腸道病毒　； 參考：《中國婦幼保健》2014年22期

【摘要】：目的:探討腸道病毒(HEV)相關小兒病毒性腦炎病原譜及分子流行病學特征,為臨床治療提供依據(jù)。方法:選取2010年1月~2012年12月珠海市各監(jiān)測點中的樣本,患兒均疑似患病毒性腦炎。病毒株均采用WTO《病毒試驗室操作手冊》檢測,腦脊液或糞便0.2 ml接種RD細胞,操作步驟按說明書進行。結果:觀察的545份樣本中,分離到HEV115株,分離率為21.1%。在375份腦脊液樣本中,分離HEV 72株,分離率為19.2%;在170份糞便樣本中,分離HEV 43株,分離率為25.3%。分離的115株病毒共涉及12個血清型,CVB3-5、E25和E30在近3年中均有檢出,且分離株最多,為絕對優(yōu)勢株。9例同時采集腦脊液和糞便樣本的患兒中,有7例在兩類樣本均分離到同一血清型病毒,CVB3和CVB5各2例,兩組比較,差異無統(tǒng)計學意義(P0.05);住院天數(shù)比較,差異有統(tǒng)計學意義(P0.05);PICS評分比較,差異有統(tǒng)計學意義(P0.05);綜合療效比較,差異有統(tǒng)計學意義(P0.01)。測序株各血清型內(nèi)nt和aa同源性分別為79.3%~99.8%和89.1%~99.9%,與各原型株的nt和aa的同源性分別為79.1%~99.90%和88.4%~99.8%。結論:由于病原體的基因重組和變異,易使其致病病原改變,各地醫(yī)務工作者對于HEV的基因變異應做好高度的準備,以防范各地的病毒性腦炎的暴發(fā)。
[Abstract]:Objective: To investigate the pathogenic spectrum and molecular epidemiological characteristics of enterovirus (HEV) related viral encephalitis in children, and to provide a basis for clinical treatment. Methods: samples from every monitoring site in Zhuhai in January 2010, ~2012, December, were suspected to be infected with viral encephalitis. The virus strains were detected by the operation manual of WTO< virus test room, cerebrospinal fluid or feces. After 0.2 ml inoculation of RD cells, the operation steps were carried out according to the instructions. Results: in the 545 samples of the observation, the HEV115 strain was isolated, the separation rate was 21.1%. in 375 CSF samples, 72 isolates were isolated and the separation rate was 19.2%; 43 HEV isolates were isolated in 170 fecal samples and 12 serotypes, CVB3-5, E2 isolated with the separation rate of 25.3%. were involved. 5 and E30 were detected in the last 3 years, and the isolates were the most. Among the children with.9 and fecal samples, 7 of the two samples were separated to the same serotype virus, 2 cases of CVB3 and CVB5, and the two groups were not statistically significant (P0.05), and the difference was statistically significant (P0.05); PICS The difference was statistically significant (P0.05), and the difference was statistically significant (P0.01). The homology of NT and AA in the serotypes of the sequenced strains was 79.3%~99.8% and 89.1%~99.9% respectively, and the homology of NT and AA in the prototypes was 79.1%~99.90% and 88.4%~ 99.8%., respectively: because of the recombinant and mutation of the pathogen, it was easy to make the mutation of the pathogen. The change of its pathogenic bacteria, medical workers everywhere should be highly prepared for the genetic variation of HEV, so as to prevent the outbreak of viral encephalitis.
【作者單位】： 廣東省珠海市第二人民醫(yī)院;
【分類號】：R725.1;R181.3

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