本文選題：ATP生物熒光法 + ATP細(xì)菌計(jì)數(shù)　； 參考：《中國(guó)消毒學(xué)雜志》2014年09期

【摘要】：目的研究ATP生物熒光法監(jiān)測(cè)物體表面染菌量的可行性。方法采用ATP生物熒光儀及其配套試劑檢測(cè)方法,對(duì)醫(yī)務(wù)人員手上污染菌量進(jìn)行監(jiān)測(cè),同時(shí)與活菌計(jì)數(shù)培養(yǎng)法作平行比較。結(jié)果洗手前后、消毒前后檢測(cè)的ATP總量、ATP細(xì)菌計(jì)數(shù)和菌落計(jì)數(shù)均有顯著性差異,手消毒后的ATP總量高于消毒前,ATP細(xì)菌計(jì)數(shù)法與比濁儀標(biāo)定的菌懸液濃度在同一數(shù)量級(jí)。結(jié)論 ATP生物熒光法可用于監(jiān)測(cè)手等物體表面的污染程度,但ATP總量測(cè)定不適用于監(jiān)測(cè)手消毒的效果。ATP細(xì)菌計(jì)數(shù)由于消除了體細(xì)胞ATP對(duì)檢測(cè)結(jié)果的影響,可以用于手消毒效果的監(jiān)測(cè)。
[Abstract]:Objective to study the feasibility of ATP biofluorescence method for monitoring bacterial contamination on the surface of objects. Methods ATP biofluorometer and its reagent were used to monitor the contamination of bacteria in the hands of medical workers, and the results were compared in parallel with the method of counting and culture of live bacteria. Results before and after hand washing, there were significant differences in total ATP bacterial count and colony count before and after disinfection. The total ATP content after hand disinfection was higher than that before disinfection by bacteria count method and turbidimeter at the same order of magnitude. Conclusion the method of ATP biofluorescence can be used to monitor the degree of contamination on the surface of hand and other objects, but the total amount of ATP is not suitable for monitoring the effect of hand disinfection. The count of ATP bacteria eliminates the influence of somatic ATP on the result of detection. It can be used to monitor the hand disinfection effect.
【作者單位】： 河北醫(yī)科大學(xué)第二醫(yī)院;
【分類號(hào)】：R187

【相似文獻(xiàn)】

相關(guān)期刊論文 前7條

1 常后嬋;鄒麗娟;戴紅霞;馮秀蘭;張靜;;應(yīng)用ATP生物熒光法動(dòng)態(tài)監(jiān)測(cè)腔鏡器械清洗環(huán)節(jié)[J];中國(guó)護(hù)理管理;2010年06期

2 顧青;方英;顧立英;吳忱姣;鐘曉琪;顧秀珍;沈哲;虞朝輝;;兩種方法刷洗內(nèi)鏡活檢孔道去污效果的對(duì)比研究[J];中華醫(yī)院感染學(xué)雜志;2010年17期

3 邢書霞;朱亭亭;張劍;張流波;;不同品牌醫(yī)用清洗劑對(duì)生物膜污染內(nèi)鏡清洗效果的比較研究[J];中國(guó)消毒學(xué)雜志;2011年04期

4 全逸先;;填補(bǔ)國(guó)際空白 我國(guó)將出臺(tái)首個(gè)醫(yī)用清洗劑國(guó)家標(biāo)準(zhǔn)——訪《醫(yī)用清洗劑衛(wèi)生標(biāo)準(zhǔn)》主要起草人張流波[J];中國(guó)衛(wèi)生標(biāo)準(zhǔn)管理;2010年03期

5 邱穎;邱賀民;李玉嬋;劉國(guó)良;;ATP發(fā)光技術(shù)快速檢測(cè)飲用水中菌落總數(shù)[J];實(shí)用預(yù)防醫(yī)學(xué);2011年02期

6 ;北京賽恩思科貿(mào)有限公司食品檢測(cè)試劑盒[J];中國(guó)食品衛(wèi)生雜志;2001年03期

7 ;北京賽恩思科貿(mào)有限公司食品檢測(cè)試劑盒[J];中國(guó)食品衛(wèi)生雜志;2001年05期

，

本文編號(hào)：1952049