本文選題：隱孢子蟲 + 腹瀉　； 參考：《中國醫(yī)科大學》2005年碩士論文

【摘要】：目的 隱孢子蟲(Cryptosporidium,CPS)是一種世界分布的人畜共患腸道寄生原蟲,是引起腹瀉的重要病原體。人體隱孢子蟲病(Cryptosporidiosis,Cs)的絕大多數(shù)臨床病例是由微小隱孢子蟲(Cryptosporidium parvum,C.p.)引起。國內(nèi)1987年韓范等在南京首次報道Cs后,全國十余個省陸續(xù)有病例報道。遼寧省至今尚未見隱孢子蟲感染的病例報道及流行病學資料。由于缺乏一種準確可靠的病原學診斷方法以及臨床醫(yī)師缺乏經(jīng)驗,經(jīng)常造成Cs的誤診和漏診,延誤了治療。因此,建立一種快速、準確、方便、有效的診斷方法對于Cs的早期診斷及治療具有重要意義。本實驗從臨床應(yīng)用角度出發(fā),進行以下研究:(1)調(diào)查沈陽地區(qū)不同人群即吸毒人群和腹瀉患者中人體隱孢子蟲感染情況,比較不同性別、不同年齡及不同職業(yè)人群感染率的差異,并同國外及全國報道的感染率比較,為流行病學研究提供資料。(2)比較碘染色、改良抗酸染色法(MAFS)及氯唑黑染色法的有效性和實用性,創(chuàng)建二步聯(lián)合染色法檢查糞便中隱孢子蟲卵囊(Cryptosporidium oocyst,CSO),為疾病診斷和流行病學調(diào)查提供方法依據(jù)。(3)采用飲水途徑給予小鼠免疫抑制劑建立隱孢子蟲感染小鼠模型的嘗試,為進一步進行隱孢子蟲的致病機理和藥物篩選等方面的研究奠定基礎(chǔ)。 材料與方法 1.小鼠隱孢子蟲感染模型及陽性對照標本的建立 10只10日齡KM小鼠(中國醫(yī)科大學實驗動物中心提供),不分雌雄,體重平均7g。小鼠糞便抗酸染色結(jié)果與已知陽性標本(解放軍軍需大學張西臣教授提供)做對照,確定陽性結(jié)果后,收集該小鼠糞便,用作本研究的陽性參考標本。 2.人體糞便標本的收集
[Abstract]:Purpose Cryptosporidium Cryptosporidium (CPS) is a worldwide parasitic protozoa of zoonotic intestinal tract, which is an important pathogen of diarrhea. The vast majority of clinical cases of CryptosporidiosisCsin human Cryptosporidium parvumum (C.p.p) are reported by Cryptosporidium parvumum. Cause. After Han Fan et al reported Cs for the first time in Nanjing in 1987, more than ten provinces reported cases one after another. There is no case report and epidemiological data of Cryptosporidium infection in Liaoning Province. Due to the lack of an accurate and reliable etiological diagnosis method and the lack of experience of clinicians, the misdiagnosis and missed diagnosis of Cs often lead to delayed treatment. Therefore, it is important to establish a rapid, accurate, convenient and effective diagnostic method for the early diagnosis and treatment of Cs. From the point of clinical application, the following research was carried out: 1) to investigate the infection of Cryptosporidium in different groups of people in Shenyang, that is, drug users and patients with diarrhea, and to compare the difference of infection rate among different sex, age and occupation. Compared with the infection rate reported abroad and nationwide, the effectiveness and practicability of iodine staining, modified acid-fast staining and chlorazolium black staining were compared. To establish a two-step combined staining method for the detection of Cryptosporidium oocystystus CSOO in feces, and to provide a method basis for disease diagnosis and epidemiological investigation) to establish a mouse model of Cryptosporidium oocyststachys infection by drinking water route with immunosuppressive agents of Cryptosporidium oocystystus. To lay a foundation for further research on the pathogenic mechanism and drug screening of Cryptosporidium. Materials and methods 1. Model of Cryptosporidium infection in mice and Establishment of positive Control specimen Ten 10-day-old km mice (provided by Center of Experimental Animals, China Medical University) had an average weight of 7 g, regardless of male and female. The results of acid-fast staining in mouse feces were compared with those of known positive specimens (provided by Professor Zhang Xichen, military supplies University of the PLA). After the positive results were confirmed, the feces of the mice were collected and used as the positive reference specimens in this study. 2. Collection of Human fecal specimens
【學位授予單位】：中國醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：R181.3

【參考文獻】

相關(guān)期刊論文 前10條

1 宮玉香,曹善青,石秀珍,韓敏,周紹礎(chǔ),郝菊花;青島地區(qū)人體隱孢子蟲感染調(diào)查[J];青島醫(yī)學院學報;1997年02期

2 黃民主,關(guān)嵐,周楚湘,李登清,胡彬;兒童腹瀉患者隱孢子蟲感染的研究[J];湖南醫(yī)科大學學報;1998年03期

3 蔡茹;李朝品;王健;許禮發(fā);賀驥;;淮南地區(qū)腹瀉患者隱孢子蟲感染的調(diào)查[J];熱帶病與寄生蟲學;2003年01期

4 曾少華,向延娥,易松林;隱孢子蟲人體呼吸道感染情況及檢驗方法的探討[J];實用預(yù)防醫(yī)學;2003年01期

5 程永璋,陳學軍,林愛芬,陸紹紅,陳睿,聞禮永,陳彩華;杭州地區(qū)人體隱孢子蟲病26例報告[J];上海醫(yī)學檢驗雜志;2000年05期

6 韓范,譚渭仙,周性蘭;南京地區(qū)人體隱孢子蟲病2例報告[J];江蘇醫(yī)藥;1987年12期

7 黃民主,關(guān)嵐,謝梅芝,李登清,周價,李祝英,劉浩,戴衛(wèi)平;長沙市某戒毒所男性吸毒人員中隱孢子蟲感染狀況的研究[J];中國公共衛(wèi)生;2003年03期

8 凌曉明,陳紅,岳偉,毛小榮,宋家駿;人體隱孢子蟲在特殊人群中的感染調(diào)查[J];中國寄生蟲病防治雜志;2001年03期

9 趙雪妮,張冬梅,張立憲,劉秀珉;哈爾濱地區(qū)嬰幼兒隱孢子蟲感染調(diào)查[J];中國寄生蟲病防治雜志;1997年03期

10 陸紹紅,林愛芬,陳睿,聞禮永,陳彩華,程永璋,陳學軍,朱冠;浙江省兒童隱孢子蟲感染的診斷及分析[J];中國人獸共患病雜志;2000年01期

，

本文編號：1894364