發(fā)布時(shí)間：2018-05-15 23:11

  本文選題：隱孢子蟲 + 腹瀉�。� 參考：《中國(guó)醫(yī)科大學(xué)》2005年碩士論文

【摘要】：目的 隱孢子蟲(Cryptosporidium,CPS)是一種世界分布的人畜共患腸道寄生原蟲,是引起腹瀉的重要病原體。人體隱孢子蟲病(Cryptosporidiosis,Cs)的絕大多數(shù)臨床病例是由微小隱孢子蟲(Cryptosporidium parvum,C.p.)引起。國(guó)內(nèi)1987年韓范等在南京首次報(bào)道Cs后,全國(guó)十余個(gè)省陸續(xù)有病例報(bào)道。遼寧省至今尚未見隱孢子蟲感染的病例報(bào)道及流行病學(xué)資料。由于缺乏一種準(zhǔn)確可靠的病原學(xué)診斷方法以及臨床醫(yī)師缺乏經(jīng)驗(yàn),經(jīng)常造成Cs的誤診和漏診,延誤了治療。因此,建立一種快速、準(zhǔn)確、方便、有效的診斷方法對(duì)于Cs的早期診斷及治療具有重要意義。本實(shí)驗(yàn)從臨床應(yīng)用角度出發(fā),進(jìn)行以下研究:(1)調(diào)查沈陽地區(qū)不同人群即吸毒人群和腹瀉患者中人體隱孢子蟲感染情況,比較不同性別、不同年齡及不同職業(yè)人群感染率的差異,并同國(guó)外及全國(guó)報(bào)道的感染率比較,為流行病學(xué)研究提供資料。(2)比較碘染色、改良抗酸染色法(MAFS)及氯唑黑染色法的有效性和實(shí)用性,創(chuàng)建二步聯(lián)合染色法檢查糞便中隱孢子蟲卵囊(Cryptosporidium oocyst,CSO),為疾病診斷和流行病學(xué)調(diào)查提供方法依據(jù)。(3)采用飲水途徑給予小鼠免疫抑制劑建立隱孢子蟲感染小鼠模型的嘗試,為進(jìn)一步進(jìn)行隱孢子蟲的致病機(jī)理和藥物篩選等方面的研究奠定基礎(chǔ)。 材料與方法 1.小鼠隱孢子蟲感染模型及陽性對(duì)照標(biāo)本的建立 10只10日齡KM小鼠(中國(guó)醫(yī)科大學(xué)實(shí)驗(yàn)動(dòng)物中心提供),不分雌雄,體重平均7g。小鼠糞便抗酸染色結(jié)果與已知陽性標(biāo)本(解放軍軍需大學(xué)張西臣教授提供)做對(duì)照,確定陽性結(jié)果后,收集該小鼠糞便,用作本研究的陽性參考標(biāo)本。 2.人體糞便標(biāo)本的收集
[Abstract]:Purpose Cryptosporidium Cryptosporidium (CPS) is a worldwide parasitic protozoa of zoonotic intestinal tract, which is an important pathogen of diarrhea. The vast majority of clinical cases of CryptosporidiosisCsin human Cryptosporidium parvumum (C.p.p) are reported by Cryptosporidium parvumum. Cause. After Han Fan et al reported Cs for the first time in Nanjing in 1987, more than ten provinces reported cases one after another. There is no case report and epidemiological data of Cryptosporidium infection in Liaoning Province. Due to the lack of an accurate and reliable etiological diagnosis method and the lack of experience of clinicians, the misdiagnosis and missed diagnosis of Cs often lead to delayed treatment. Therefore, it is important to establish a rapid, accurate, convenient and effective diagnostic method for the early diagnosis and treatment of Cs. From the point of clinical application, the following research was carried out: 1) to investigate the infection of Cryptosporidium in different groups of people in Shenyang, that is, drug users and patients with diarrhea, and to compare the difference of infection rate among different sex, age and occupation. Compared with the infection rate reported abroad and nationwide, the effectiveness and practicability of iodine staining, modified acid-fast staining and chlorazolium black staining were compared. To establish a two-step combined staining method for the detection of Cryptosporidium oocystystus CSOO in feces, and to provide a method basis for disease diagnosis and epidemiological investigation) to establish a mouse model of Cryptosporidium oocyststachys infection by drinking water route with immunosuppressive agents of Cryptosporidium oocystystus. To lay a foundation for further research on the pathogenic mechanism and drug screening of Cryptosporidium. Materials and methods 1. Model of Cryptosporidium infection in mice and Establishment of positive Control specimen Ten 10-day-old km mice (provided by Center of Experimental Animals, China Medical University) had an average weight of 7 g, regardless of male and female. The results of acid-fast staining in mouse feces were compared with those of known positive specimens (provided by Professor Zhang Xichen, military supplies University of the PLA). After the positive results were confirmed, the feces of the mice were collected and used as the positive reference specimens in this study. 2. Collection of Human fecal specimens
【學(xué)位授予單位】：中國(guó)醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R181.3

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本文編號(hào)：1894364