本文選題：手足口病 + 柯薩奇病毒�。� 參考：《中國衛(wèi)生檢驗雜志》2014年11期

【摘要】：目的研究2013年杭州地區(qū)兒童手足口病流行特征,比較2種實驗室檢測方法的檢出率。方法搜集杭州流行期間臨床疑似手足口病的患兒283名,同時采集咽拭子或肛拭子標(biāo)本和急性期血液樣本,熒光定量PCR測定咽拭子或肛拭子標(biāo)本中的腸道病毒;ELISA測定急性期血清中EV71型IgM抗體和CA16型IgM抗體。整理2011年-2013年本院HFMD的數(shù)據(jù),統(tǒng)計不同類型腸道病毒的分布比例和流行趨勢。結(jié)果近3年EV71和CA16有下降趨勢,其他EV比例2013年明顯上升。2013年發(fā)病年齡在0歲~3歲的患兒占86.22%,男女比例為1.44∶1。PCR的總陽性率為84.10%,EV71、CA16和其他EV分別占27.21%(77/283),4.59%(13/283)和52.30%(148/283);ELISA檢測的總陽性率為32.51%(92/283),其中EV71和CA16-IgM分別占29.33%(83/283)和3.18%(9/283)。兩種方法結(jié)果的配對χ2檢驗P0.05。結(jié)論 2013年春夏季杭州地區(qū)手足口病流行特點以其他EV為主,以發(fā)熱等輕癥為主;引起重癥HFMD以EV71型為主。PCR與ELISA對EV71和CA16的檢出率差異無統(tǒng)計學(xué)意義,ELISA適合基層推廣。
[Abstract]:Objective to study the epidemic characteristics of hand, foot and mouth disease (HFMD) in children in Hangzhou in 2013 and to compare the detection rate of two laboratory methods. Methods 283 children with suspected HFMD during epidemic period in Hangzhou were collected, and throat swabs or anal swabs and blood samples in acute phase were collected. Serum EV71 type IgM antibody and CA16 type IgM antibody were detected by fluorescence quantitative PCR in throat swabs or anal swabs by Elisa. The data of HFMD from 2011 to 2013 were collected and the distribution and epidemic trend of different types of enterovirus were analyzed. Results EV71 and CA16 showed a downward trend in the last three years. The proportion of other EV increased significantly in 2013. In 2013, 86.22 cases were aged 0 to 3 years old, and the total positive rate of EV71CA16 and other EV levels was 27.2177 / 283C / 283Q / 13283 and 52.30148283P / 92833, respectively. The total positive rates of EV71 and CA16-IgM were 29.3383 / 283and 3.18 / 9283respectively, respectively. The positive rate of EV71CA16 and other EV was 27.21 / 283and 52.30148283P / 928 / 283respectively, and the positive rates of EV71 and CA16-IgM were 29.3383 / 283and 3.18 / 9283respectively. The paired 蠂 2 test of the results of the two methods (P 0.05). Conclusion the epidemic characteristics of HFMD in Hangzhou in spring and summer 2013 are mainly other EV and mild fever, and the detection rate of EV71 and CA16 is not significantly different between ELISA and EV71.
【作者單位】： 杭州市兒童醫(yī)院檢驗科;杭州市兒童醫(yī)院感染科;
【基金】：浙江省自然科學(xué)基金(LY13H040007) 浙江省醫(yī)藥衛(wèi)生科技計劃(2012KYB159)
【分類號】：R181.3

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 周立民;熊潤頻;;阜陽EV71疫情追蹤[J];w，

本文編號：1829534