本文選題：卡介苗 + 細(xì)胞免疫�。� 參考：《暨南大學(xué)》2006年碩士論文

【摘要】：目的 分析漢族嬰兒接種卡介苗(BCG)后細(xì)胞免疫功能變化，探討DC-SIGN編碼基因第4外顯子多拷貝重復(fù)序列區(qū)(簡稱DC-SIGN重復(fù)序列區(qū)基因)多態(tài)性與BCG接種效果之間的關(guān)系，為結(jié)核感染的防治提供新途徑。 方法 收集150例BCG接種后PPD陽性漢族嬰兒(接種成功組)和50例BCG接種后PPD陰性漢族嬰兒(接種失敗組)的外周靜脈血，ELISA法測定血清細(xì)胞因子IFN-γ和IL-10的含量，評價(jià)細(xì)胞免疫功能；應(yīng)用分子生物學(xué)技術(shù)(PCR擴(kuò)增、瓊脂糖凝膠電泳)分析DC-SIGN重復(fù)序列區(qū)基因多態(tài)性，探討B(tài)CG接種效果與DC-SIGN碼基因多態(tài)性的關(guān)系。 結(jié)果 ①接種成功組和接種失敗組血清細(xì)胞因子IFN-γ含量分別為49.78±12.20pg／ml和38.08±7.68pg／ml，差異具有統(tǒng)計(jì)學(xué)意義(P=0.001)；血清IL-10含量分別為3.60±2.00pg／ml和5.71±2.71pg／m，差異具有統(tǒng)計(jì)學(xué)意義(P=0.008)。②200例BCG接種漢族嬰兒(包括接種成功和失敗組)中，存在6和7型2種DC-SIGN重復(fù)序列區(qū)基因型，分別組成7／7野生型純合子(197例，占98.5％)或6／7變異型雜合子(3例，，占1.5％)。③150例接種成功組嬰兒的DC-SIGN重復(fù)序列區(qū)基因均為7／7純合子(100％)；50例接種失敗組的DC-SIGN重復(fù)序列區(qū)基因中，47例為7／7純合子(94.0％)，3例為6／7雜合子(6.0％)。兩組比較，差異有統(tǒng)計(jì)學(xué)意義(Fisher's精確概率法，P=0.015) 。 結(jié)論 ①BCG接種成功(PPD陽性)嬰兒的細(xì)胞免疫功能增強(qiáng)。②漢族嬰兒DC-SIGN重復(fù)序列區(qū)基因以7／7野生型純合子為主；BCG接種失敗漢族嬰兒存在6／7變異型雜合子。③DC-SIGN重復(fù)序列區(qū)基因變異可能是導(dǎo)致BCG接種失敗的原因之一。
[Abstract]:Objective to analyze the changes of cellular immune function in infants of Han nationality after BCG inoculation, and to explore the relationship between the polymorphism of DC-SIGN repeat region in exon 4 of DC-SIGN coding gene and the effect of BCG inoculation. To provide a new way for the prevention and treatment of tuberculosis infection. Methods Serum levels of cytokines IFN- 緯 and IL-10 were determined by Elisa in 150 PPD positive Han infants (successful group) and 50 PPD negative Han infants (failed group) after BCG inoculation. To evaluate the cellular immune function, to analyze the polymorphism of DC-SIGN repeat region by molecular biological technique and agarose gel electrophoresis, and to explore the relationship between the effect of BCG inoculation and the polymorphism of DC-SIGN code gene. Results 1the levels of serum cytokine IFN- 緯 in successful inoculation group and failed inoculation group were 49.78 鹵12.20pg/ml and 38.08 鹵7.68 PG / ml, respectively, the difference was statistically significant (P < 0.01), and the serum IL-10 content was 3.60 鹵2.00pg/ml and 5.71 鹵2.71pggm-1, respectively. The difference was statistically significant in BCG inoculated Han nationality infants. In children (including successful and failed vaccination groups), There were two genotypes of 6 and 7 DC-SIGN repeats, which consisted of 7 / 7 wild type homozygotes in 197 cases (98.5%) or 6 / 7 variant heterozygotes in 3 cases, respectively. The DC-SIGN repeat region genes in 1.5% and 3150 cases of successful inoculation group were 7 / 7 homozygote and 50 cases of failed inoculation group. 47 cases were 7 / 7 homozygous 7% homozygous 3 cases were 6 / 7 heterozygote 6.0g. The difference between the two groups was statistically significant (P 0.015). Conclusion the cellular immune function of infants with successful 1BCG inoculation is enhanced. 2 the gene of DC-SIGN repeat region in Han infants is mainly 7 / 7 wild type homozygote. There is 6 / 7 heterozygote. 3DC-SIGN heavy in Han infants. The gene variation of complex sequence region may be one of the reasons for the failure of BCG inoculation.
【學(xué)位授予單位】：暨南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R186

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