本文選題：乙肝感染標(biāo)志 + 血清流行病學(xué)　； 參考：《河北醫(yī)科大學(xué)》2005年碩士論文

【摘要】：目的:了解山東濟(jì)陽縣15~40 歲人群HBV 感染標(biāo)志物的分布情況;觀察國產(chǎn)10μg/ml 酵母乙肝疫苗不同免疫程式接種成人的免疫效果;分析單純核心抗體陽性血清HBV DNA 陽性率。 方法:2003 年11 月,在山東省濟(jì)陽縣整群隨機(jī)抽取某鎮(zhèn)20 個村,對15~40 歲人群采集靜脈血。用固相放射免疫(SPRIA)法檢測HBsAg、抗-HBs 和抗-HBc 等HBV 感染指標(biāo),用酶聯(lián)免疫(EIA)法檢測HBeAg 和抗-HBe 指標(biāo),分析當(dāng)?shù)氐腍BV 流行情況。 將篩檢出的易感者隨機(jī)整群分為兩組,分別按0-1-6 個月程序和0-6 個月程序接種10μg/ml 酵母乙肝疫苗。全程免疫后1 個月,SPRIA 法定量檢測抗體水平。 對收集的資料用Access 建立數(shù)據(jù)庫,采用SAS8.0 進(jìn)行統(tǒng)計學(xué)分析。 對單獨(dú)核心抗體陽性者用PCR 法檢測HBV DNA。實驗中以加熱法提取血清中的HBV DNA,作為模板,擴(kuò)增出S區(qū)序列,電泳鑒定。 結(jié)果:橫斷面調(diào)查顯示,15~40 歲人群HBsAg 流行率、抗-HBs 流行率、抗-HBc 流行率和HBV 總感染率分別為6.52%、24.77%、30.99%和43.63%。HBsAg 流行率隨年齡的增長而升高(P0.05)。HBeAg 的流行率隨年齡的增長而逐漸
[Abstract]:Objective : To investigate the distribution of HBV infection markers in people aged 15 to 40 years in Jiyang county , Shandong province , and observe the immune effect of 10 渭g / ml yeast hepatitis B vaccine in adults ; and analyze the positive rate of HBV DNA in the positive serum of pure core antibody .








Methods : In November 2003 , 20 villages in a town were randomly selected in Jiyang County , Shandong Province . Venous blood was collected from 15 - 40 years old . HBV infection indexes such as HBsAg , anti - HBs and anti - HBV were detected by solid - phase radioimmunoassay ( SPRIA ) . HBeAg and anti - HBe indexes were detected by EIA . The local HBV prevalence was analyzed .








The susceptible subjects were randomly divided into two groups : 0 - 1 - 6 - month procedure and 0 - 6 - month routine inoculation with 10 渭g / ml yeast hepatitis B vaccine . After the whole course of immunization , the antibody level was detected by SPRIA .








The collected data was established in Access database , and SAS 8.0 was used for statistical analysis .








HBV DNA was detected by polymerase chain reaction ( PCR ) method . HBV DNA was extracted from serum by heating method . The S - region sequence was amplified by electrophoresis and identified by electrophoresis .








Results : The prevalence rate of HBsAg , the prevalence of anti - HBs , the prevalence and the total HBV infection rate were 6.52 % , 24.77 % , 30.99 % and 43.63 % , respectively . The prevalence of HBsAg increased with age ( P0.05 ) . The prevalence of HBeAg increased with age .

【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R181.3;R186

【共引文獻(xiàn)】

相關(guān)期刊論文 前2條

1 陳仕珠;;重點(diǎn)、高危和特殊人群之乙肝免疫[J];世界華人消化雜志;2006年27期

2 Greet Boland;Jan van Hattum;Gijsbert C. de Gast;;Is a low dose of hepatitis B vaccine enough for a rapid vaccination scheme?[J];World Journal of Gastroenterology;2003年10期

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本文編號：1747843