發(fā)布時間：2018-04-12 19:48

  本文選題：庫波熱病毒 + 安哥拉��； 參考：《病毒學報》2017年03期

【摘要】：針對西非安哥拉回國的不明原因發(fā)熱病人,篩查其可能攜帶的傳染病病原體。采用熒光PCR的方法對口岸重點關注的黃熱病毒、寨卡病毒、登革病毒等病原體進行篩查;采用深度測序的方法,搜索病毒庫、細菌庫、寄生蟲庫,進行序列比對篩查各類病原體,采用全基因組測序的方法獲得病原體全基因組序列。2017年1月2日凌晨1點,上海出入境檢驗檢疫局國家國境口岸衛(wèi)生監(jiān)督檢測重點實驗室接到一份緊急樣本,樣本來自1名安哥拉回國的28歲男子。該男子在安哥拉當?shù)蒯t(yī)院初篩黃熱病陽性,該男子要求自行回國治療。針對病人的血液樣本和尿液樣本,開展黃熱病毒、登革病毒、寨卡病毒、裂谷熱病毒、基孔肯雅病毒、西尼羅病毒、流行性乙型腦炎病毒、瘧原蟲等多種蟲媒病原體的熒光PCR檢測,結果顯示陰性。通過對血液樣本的深度測序發(fā)現(xiàn),該病人感染了一種新型的彈狀病毒,中文命名為庫波熱病毒,并獲得了庫波熱病毒全基因組序列,與尼日利亞發(fā)現(xiàn)的庫波熱病毒比較,同源性為96%。
[Abstract]:Screening of infectious disease pathogens for unknown fever patients returning from Angola in West Africa.Fluorescent PCR was used to screen yellow fever virus, Zika virus, dengue virus and so on, and deep sequencing method was used to search virus library, bacterial bank, parasite bank, etc.Sequence alignment was carried out to screen all kinds of pathogens, and the whole genome sequence was obtained by whole genome sequencing. 1: 00 am on January 2, 2017,Shanghai Entry-Exit Inspection and Quarantine Bureau has received an emergency sample from a 28-year-old man who returned home from Angola.The man was tested positive for yellow fever at a local hospital in Angola and asked to return home for treatment.Yellow fever virus, dengue virus, Zika virus, Rift Valley virus, Kikungunya virus, West Nile virus, Japanese encephalitis virus,Fluorescent PCR analysis of malaria parasite and other arbogenic pathogens showed negative results.By deep sequencing of blood samples, it was found that the patient was infected with a new type of slug virus, named Kubovirus in Chinese, and obtained the whole genome sequence of Kubovirus, which was compared with the Kubovirus found in Nigeria.The homology is 96.
【作者單位】： 上海出入境檢驗檢疫局;軍事醫(yī)學科學院微生物流行病研究所;
【基金】：國內合作項目(項目號:15495800400),題目:登革熱等重要蟲媒傳染病快速檢測關鍵技術研究與防控體系構建
【分類號】：R185
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本文編號：1741169