本文選題：肝炎　切入點(diǎn)：丙型　出處：《中華疾病控制雜志》2014年04期 　論文類(lèi)型：期刊論文

【摘要】：目的探討丙型肝炎病毒(hepatitis C virus,HCV)F基因區(qū)變異的臨床意義。方法以78例未治療慢性丙型肝炎患者為研究對(duì)象,采用單鏈構(gòu)象多態(tài)性分析對(duì)HCV-F基因區(qū)進(jìn)行準(zhǔn)種分析,實(shí)時(shí)熒光定量聚合酶鏈反應(yīng)對(duì)HCV RNA進(jìn)行定量檢測(cè),利用酶聯(lián)免疫吸附試驗(yàn)分別對(duì)患者血清中的抗F蛋白抗體、Th1(IL-12,IFN-γ)和Th2(IL-5,IL-10)細(xì)胞因子水平進(jìn)行檢測(cè)分析。結(jié)果 78例慢性丙型肝炎患者中有46例為抗HCV-F抗體陽(yáng)性,陽(yáng)性率為59%。HCV1b亞型患者F基因區(qū)準(zhǔn)種數(shù)目高于非HCV1b亞型(P=0.002)。F基因區(qū)SSCP條帶數(shù)與HCV RNA載量及ALT/AST水平呈正相關(guān)(均有P0.05)。另外,與F基因準(zhǔn)種數(shù)量≤2的患者相比,F基因準(zhǔn)種數(shù)量2的患者血清中,Th1細(xì)胞因子IL-12、IFN-γ水平降低(P=0.023,P=0.010),Th2細(xì)胞因子IL-5、IL-10水平顯著升高(P=0.024,P=0.012)。結(jié)論 HCV-F基因區(qū)準(zhǔn)種多樣性與病毒復(fù)制、肝損傷和機(jī)體Th2優(yōu)勢(shì)應(yīng)答有關(guān),具有一定的臨床意義。
[Abstract]:Objective to investigate the clinical significance of HCV-F gene mutation in hepatitis C virus hepatitis C. methods 78 patients with untreated chronic hepatitis C were studied by single strand conformation polymorphism (SSCP). Real-time fluorescence quantitative polymerase chain reaction was used for quantitative detection of HCV RNA. Elisa was used to detect and analyze the levels of cytokines of anti-F protein antibody Th _ 1T _ (12) IFN- 緯) and Th _ (2) IL-5 ~ (5) IL-10) in serum of patients with chronic hepatitis C. results 46 of 78 patients with chronic hepatitis C were positive for anti HCV-F antibody. The positive rate was that the number of F gene quasispecies in 59%.HCV1b subtype was higher than that in non HCV1b subtype P0. 002. F gene SSCP bands were positively correlated with HCV RNA load and ALT/AST level (P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P < 0. 05). Compared with the patients with F gene quasispecies number 鈮，

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