發(fā)布時(shí)間：2018-03-12 06:36

  本文選題：恙蟲病東方體　切入點(diǎn)：嚙齒動(dòng)物　出處：《中國人民解放軍軍事醫(yī)學(xué)科學(xué)院》2007年碩士論文　論文類型：學(xué)位論文

【摘要】： 恙蟲病是經(jīng)恙螨叮咬傳播的一種自然疫源性疾病，其病原體為恙蟲病東方體(Orientia tsutsugamushi，Ot)。長期以來，恙蟲病僅在我國長江以南廣大地區(qū)流行，自1986年在長江以北的山東、江蘇地區(qū)發(fā)現(xiàn)恙蟲病流行后，疫區(qū)不斷向北、向西擴(kuò)展，嚴(yán)重威脅人民群眾健康。然而內(nèi)蒙古地區(qū)至今尚無該病報(bào)道，新疆僅有一篇Ot血清學(xué)調(diào)查報(bào)告。本研究的目的是調(diào)查內(nèi)蒙古、新疆地區(qū)是否存在Ot自然感染，并探討這兩個(gè)地區(qū)Ot與其他地區(qū)Ot基因序列之間的變異和親緣關(guān)系。 在內(nèi)蒙古、新疆地區(qū)選點(diǎn)，應(yīng)用間接免疫熒光法(IFA)檢測Ot-IgG抗體，結(jié)果顯示兩個(gè)地區(qū)人群血清Ot-IgG抗體陽性率分別為11.11％(13／117)和11.56％(34／294)。多因素Logistic回歸分析結(jié)果顯示，男性感染Ot的相對(duì)危險(xiǎn)度高于女性(OR=7.150)，烏魯木齊市烏拉泊三隊(duì)感染Ot的相對(duì)危險(xiǎn)度高于米泉市三道壩鎮(zhèn)(OR=9.698)。 采用巢式PCR方法對(duì)內(nèi)蒙古、新疆地區(qū)鼠類脾臟進(jìn)行檢測，，Ot感染率分別為3.57％和2.50％；在內(nèi)蒙古收集的168只鼠中，黑線姬鼠占34.5％，且有2只鼠Ot-Sta56基因擴(kuò)增陽性，提示黑線姬鼠為當(dāng)?shù)貎?yōu)勢(shì)帶菌鼠；恙螨調(diào)查顯示，內(nèi)蒙古地區(qū)鼠類帶螨率為6.82％，恙螨指數(shù)為0.49，在收集到的108只恙螨中，居中纖恙螨104只，無前恙螨屬恙螨4只，提示當(dāng)?shù)?月份鼠體外以居中纖恙螨為優(yōu)勢(shì)螨種。 將內(nèi)蒙古、新疆地區(qū)擴(kuò)增陽性標(biāo)本的Ot-Sta56基因序列與收集到的其他地區(qū)Ot進(jìn)行比較，結(jié)果顯示內(nèi)蒙古N59、N69、新疆X33及廣東、東北分離株屬Karp型；內(nèi)蒙古N90屬Oishi型；山東分離株和病人焦痂屬Kawasaki型；內(nèi)蒙古N65、N88處于獨(dú)立的分支上。另外，對(duì)N65 Ot-Sta56全基因序列分析結(jié)果進(jìn)一步支持了上述結(jié)論。提示N65 Ot-Sta56基因序列發(fā)生了較大變異。 本研究證實(shí)內(nèi)蒙古、新疆部分地區(qū)人群、鼠類中存在Ot自然感染，并且內(nèi)蒙古地區(qū)部分Ot-Sta56基因序列發(fā)生較大變異。
[Abstract]:Tsutsugamushi typhus is a natural disease transmitted by chigger mites. Its pathogen is Orientia tsugamushius Otchus. For a long time, tsutsugamus tsutsugamushius has been prevalent only in large areas south of the Yangtze River in China. Since 1986, tsutsugamus tsutsugamus has been prevalent in Shandong, north of the Yangtze River. After the epidemic of tsutsugamushi disease was discovered in Jiangsu, the epidemic area continued to expand northward and westward, seriously threatening the health of the people. However, there has been no report of the disease in Inner Mongolia so far. The purpose of this study was to investigate whether there is any natural infection of Ot in Inner Mongolia and Xinjiang, and to explore the variation and genetic relationship between the Ot gene sequences of these two regions and other regions. In Inner Mongolia and Xinjiang, indirect immunofluorescence assay was used to detect Ot-IgG antibody. The results showed that the positive rates of serum Ot-IgG antibody in the two regions were 11.1113 / 117) and 11.56% / 294% respectively. The results of multivariate Logistic regression analysis showed that. The relative risk of infection of Ot in males was higher than that in females, and the relative risk of infection of Ot in three teams of Wulapo in Urumqi was higher than that in Oran 9.698in Sandaoba town of Miquan. The infection rate of Ot was 3.57% and 2.50 respectively in the spleen of mice in Inner Mongolia and Xinjiang by nested PCR method, 34.5% of them were Apodemus agrarius collected in Inner Mongolia, and 2 of them were positive for Ot-Sta56 gene amplification. The results indicated that Apodemus agrarius was the dominant local carrying mite, and the rate of carrying mites was 6.82and the index of chigger mites was 0.49 in Inner Mongolia. Among 108 chigger mites collected, 104 were intermediate chigger mites, 4 were chigger mites without the genus chigger mites. It suggested that in September, the dominant mites were chigger mites. The Ot-Sta56 gene sequences of amplified positive samples in Inner Mongolia and Xinjiang were compared with those in other regions. The results showed that N59 N69, X33 and Guangdong in Inner Mongolia belonged to Karp type, N90 belonged to Oishi type in Inner Mongolia. Shandong isolate and patient scab belong to Kawasaki type, and N65 Ot-Sta56 N88 is an independent branch in Inner Mongolia. In addition, the analysis of N65 Ot-Sta56 gene sequence further supports the above conclusion, which suggests that the N65 Ot-Sta56 gene sequence has changed greatly. In this study, it was confirmed that there was a natural infection of ant in population and rodents in Inner Mongolia and Xinjiang, and some Ot-Sta56 gene sequences were changed greatly in Inner Mongolia.
【學(xué)位授予單位】：中國人民解放軍軍事醫(yī)學(xué)科學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R181.3;R513.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王敦清;我國恙蟲病媒介的生物系統(tǒng)分類問題[J];地方病通報(bào);1988年03期

2 薛家芹,明錦,楊會(huì)利,王顯軍,孫桐,馮開軍;山東南部再次發(fā)生恙蟲病流行的調(diào)查[J];疾病監(jiān)測;2000年10期

3 汪昭旺,劉文東,李正奎,朱洪博;肥城市首次恙蟲病暴發(fā)流行的調(diào)查分析[J];疾病監(jiān)測;2001年08期

4 吳光華;我國恙蟲病的流行病學(xué)特點(diǎn)與防治策略[J];中國公共衛(wèi)生;2000年09期

5 劉運(yùn)喜,趙仲堂,吳欽永,楊占清,張景蘭,徐建江,彭佐林,苗仲水;山東地區(qū)秋冬型恙蟲病傳播媒介的調(diào)查研究[J];現(xiàn)代預(yù)防醫(yī)學(xué);2004年05期

6 黎家燦，鄭小英，倪宏，陳添勝;地里纖恙螨紅、白體色體內(nèi)恙蟲病立克次體的聚合酶鏈反應(yīng)技術(shù)檢測及其傳病作用[J];中國人獸共患病雜志;1994年03期

7 郭恒彬，吳光華，唐家琪，李先富，于明明，張?jiān)�，潘秀珍，李越�?應(yīng)用NPCR發(fā)現(xiàn)我國Kawasaki型恙蟲病立克次體[J];中國人獸共患病雜志;1995年02期

8 鄭小英，黎家燦，倪宏，陳添勝，陳成福;恙螨體內(nèi)恙蟲病立克次體動(dòng)態(tài)變化的實(shí)驗(yàn)研究[J];中國人獸共患病雜志;1995年03期

9 郭恒彬，吳光華，潘秀珍，李先富，唐家琪，張?jiān)�，李越�?用PCR檢測現(xiàn)場單個(gè)小盾纖恙螨體內(nèi)恙蟲病立克次體的研究[J];中國人獸共患病雜志;1996年01期

10 袁慶虹,馮錫光,米竹青,張海林,自登云,陳淵民;云南省賓川縣恙蟲病暴發(fā)流行的調(diào)查[J];中國人獸共患病雜志;1999年01期

本文編號(hào)：1600367