本文選題：超廣譜β-內(nèi)酰胺酶(ESBLs)　切入點：腸桿菌科細菌　出處：《中國公共衛(wèi)生》2017年03期 　論文類型：期刊論文

【摘要】：目的了解產(chǎn)超廣譜β-內(nèi)酰胺酶(ESBLs)腸桿菌科細菌在山東某生豬養(yǎng)殖縣農(nóng)村居民中的流行現(xiàn)狀及耐藥情況,為控制抗生素耐藥提供參考依據(jù)。方法于2016年7月采用隨機抽樣方法在山東省東部某畜牧養(yǎng)殖縣抽取4個村160名農(nóng)村常住居民作為研究對象,使用e Swab采樣管采集其糞便樣本,接種于ESBLs篩選培養(yǎng)基,質(zhì)譜鑒定菌株型別,根據(jù)2015版美國臨床和實驗室標準進行ESBLs確證試驗和藥物敏感性試驗。結(jié)果山東某生豬養(yǎng)殖縣160份農(nóng)村居民的糞便樣本中有132份產(chǎn)ESBLs,攜帶率為82.5%;132份陽性標本中共包含產(chǎn)ESBLs的大腸埃希菌184株和肺炎克雷伯菌23株,共207株菌株;產(chǎn)ESBLs的大腸埃希菌和肺炎克雷伯菌對頭孢噻肟、四環(huán)素、復方新諾明、環(huán)丙沙星、氟苯尼考、慶大霉素、哌拉西林/他唑巴坦、磷霉素、阿莫西林/克拉維酸、呋喃妥因、阿米卡星、頭孢西丁、美羅培南、替加環(huán)素的耐藥率分別為100.0%和100.0%、87.0%和95.7%、82.1%和100.0%、64.7%和30.4%、56.5%和43.5%、53.8%和60.9%、31.0%和78.3%、29.9%和69.6%、20.1%和52.2%、13.0%和30.4%、7.1%和0、4.9%和0、0和0、0和56.5%;產(chǎn)ESBLs的大腸埃希菌和肺炎克雷伯菌中,耐1~2、3~5、6~8和≥9種藥物的比例分別為7.3%、50.7%、39.6%和2.4%。結(jié)論產(chǎn)ESBLs腸桿菌科細菌在山東農(nóng)村生豬養(yǎng)殖地區(qū)的攜帶率較高,對頭孢噻肟、四環(huán)素、復方新諾明等藥物耐藥性較強。
[Abstract]:Objective to investigate the prevalence and drug resistance of extended-spectrum 尾 -lactamases (ESBLs) bacteria in rural residents of a live pig breeding county in Shandong province. Methods in July 2016, a random sampling method was used to sample 160 rural residents from 4 villages in a livestock farming county in eastern Shandong Province. The fecal samples were collected by e Swab sampling tube. Strain types were identified by mass spectrometry after inoculation in ESBLs screening medium. ESBLs confirmatory test and drug sensitivity test were carried out according to the 2015 US clinical and laboratory standards. Results 132 feces samples from 160 rural residents in a live pig breeding county in Shandong province produced ESBLs.The carrying rate was 82.5% and 132 positive samples. #number0# strains of Escherichia coli and 23 strains of Klebsiella pneumoniae were found to produce ESBLs. ESBLs producing Escherichia coli and Klebsiella pneumoniae against cefotaxime, tetracycline, compound sulfamide, ciprofloxacin, florfenicol, gentamicin, piperacillin / tazobactam, fosfomycin, amoxicillin / clavulanic acid, The drug resistance rates of nitrofurantoin, amikacin, cefoxitin, meropenem and tegacycline were 100.0% and 100.00.087.0% and 95.77.0%, 82.1% and 100.064.7% and 30.4% and 56.5% and 43.53.5% and 43.53.5% and 43.53.5% and 60.931% and 78.329.9% and 69.620.1% and 59.620.1% and 30.47.1% and 30.47.1% and 04.9% and 0, 00, 0 and 56.5of ESBLs, respectively, of Escherichia coli and Klebsiella pneumoniae of ESBLs, The rates of resistance to 1, 2, 2, 3, 3, 5, 5, 5, 5, 5, 8, and 鈮，

本文編號：1595768