本文選題：酸性氧化電位水　切入點：消毒　出處：《浙江大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 評價內(nèi)窺鏡全自動清洗消毒機產(chǎn)生流動酸性氧化電位水進(jìn)行滅菌的效果,嘗試確定流動酸性氧化電位水進(jìn)行胃鏡消毒的最短時間。 材料和方法 1 材料 枯草桿菌黑色變種芽孢(ATCC9372)、內(nèi)窺鏡全自動清洗消毒機、日本Olympus240型電子胃鏡及MTN-HT型一次性內(nèi)窺鏡活檢鉗、0.07%卵磷脂,0.5%硫代硫酸鈉和2%吐溫—80磷酸鹽緩沖液、一次性使用平皿、GNP-9160型隔水式恒溫培養(yǎng)箱、DK—8D型電熱恒溫水槽等。 2 實驗步驟: 2.1 中和劑鑒定試驗 試驗設(shè)平行6組,即消毒劑+菌懸液、消毒劑+菌懸液+中和劑、中和劑+菌懸液、消毒劑+中和劑+菌懸液、稀釋液+菌懸液、稀釋液+中和劑+培養(yǎng)基,按懸液定量殺菌試驗方法進(jìn)行試驗。 2.2 懸液定量殺菌試驗 將酸性氧化電位水經(jīng)3%牛血清白蛋白干擾的枯草桿菌黑色變種芽孢菌懸液混勻,作用至預(yù)定時間,取最終樣液1.0ml液滴于平皿,然后傾注15ml胰蛋白胨大豆瓊脂培養(yǎng)基,置于37℃培養(yǎng)箱內(nèi)培養(yǎng)72h,計數(shù)菌落數(shù),計算殺滅對數(shù)值。 2.3 模擬試驗
[Abstract]:Purpose. To evaluate the effect of automatic cleaning and disinfecting machine for endoscope to produce flowing acidic oxidizing potential water for sterilization, and to try to determine the shortest time for gastroscope disinfection with flowing acidic oxidizing potential water. Materials and methods. 1 material. The spores of Bacillus subtilis var. Niger ATCC 9372, endoscope automatic cleaning and disinfecting machine, Japanese Olympus240 electronic gastroscope and MTN-HT disposable endoscope biopsy forceps were 0.07% lecithin 0.5% sodium thiosulfate and 2% Tween-80 phosphate buffer. One-time use of flat plate GNP-9160 water-proof constant temperature incubator DK-8 D electrothermal constant temperature tank and so on. 2 Experimental steps:. 2.1 neutralizer identification test. The experiment was divided into 6 groups: disinfectant suspension, disinfectant suspension neutralizer, neutralizer suspension, disinfectant neutralizer suspension, diluent suspension, diluent neutralizer medium. The test was carried out according to suspension quantitative germicidal test method. 2.2 suspension quantitative bactericidal test. The acidic oxidizing potential water was mixed with Bacillus subtilis var. Niger suspension interfered by 3% bovine serum albumin (BSA), and the final sample solution of 1.0 ml liquid was dripped onto the plate at a predetermined time, and then 15 ml tryptone soybean Agar medium was poured into the medium. Cultured in 37 鈩，

本文編號：1574705