目的探討腸道微生物變化與復發(fā)性口腔潰瘍（ROU）的相關性,進而確定腸道微生物變化是否是導致ROU的致病因素之一。方法選取8名經常發(fā)作ROU作為實驗組（HR）; 14名健康志愿者作為對照組（HN）。對糞便進行宏基因組測序,同時用ELISA檢測糞便中炎癥因子白細胞介素（IL）-10和IL-22。結果在門水平上,HR中厚壁菌門的豐度高于HN（P<0.05）。在屬水平上,HN中Escherichia/Shigella和雙歧桿菌的豐度高于HR（P<0.05）。HR組腸球菌的豐度高于HN組（P<0.05）。糞便中IL-10水平在HN、HR組中相似,兩組間差異無統計學意義（P>0.05）。HR組IL-22水平高于HN組,兩組間差異有統計學意義（P<0.05）。單因素Logistic回歸分析顯示IL-22是ROU的危險因素。多因素Logistic回歸分析顯示,雙歧桿菌豐度可能是ROU的保護因素,糞便中IL-22可能是危險因素。結論 ROU患者糞便中IL-22與腸道菌群之間存在相互作用,雙歧桿菌屬豐度顯著降低及IL-22水平的升高參與了ROU的發(fā)生,且IL-22在ROU發(fā)生... 

【文章來源】：解剖學報. 2020,51(04)北大核心

【文章頁數】：10 頁

【文章目錄】：
Objects and Methods
    1. Experimental object and grouping
        1.1 Inclusion criteria:
        1.2 Exclusion criteria:
    2. Feces sample collection
    3. High-throughput sequencing
        3.1 Sample collection:
        3.2 DNA extraction:
        3.3 Library preparation and Illumina
    4. Taxonomic assignment of bacterial 16S rDNA sequencesand data analysis
    5. ELISA detecting the expression of IL-10 and IL-22 in faeces
    6.Data analysis
Results
    1.Sequencing data evaluation
    2.Alpha-diversity analysis
    3.Intestinal microbial community structura features and core flora between the HR and the HN
    4.Principal component analysis based on OTUs
    5. Uni Fracβdiversity analysis
    6. Functional abundance comparison and difference analysis between the two groups
    7. Expression of IL-10 and IL-22 in faeces
Discussion


【參考文獻】：
期刊論文
[1]Interleukin-22 receptor 1 is expressed in multinucleated giant cells:A study on intestinal tuberculosis and Crohn’s disease[J]. Zi-Qi Yu,Wen-Fei Wang,You-Chao Dai,Xin-Chun Chen,Jian-Yong Chen.  World Journal of Gastroenterology. 2019(20)



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