【摘要】：目的探索改善鈦種植體表面處理的新方法,提高種植體植入后成骨效率。方法將RGD肽與殼聚糖(CS)通過(guò)酰化反應(yīng)發(fā)生偶聯(lián)形成RGD-CS,采用復(fù)凝聚法制備RGD-CS/pDNA復(fù)合體,并將RGD-CS/pDNA復(fù)合體接枝到經(jīng)物理、生化處理后的純鈦表面。采用紅外光譜儀和元素分析儀對(duì)RGD-CS進(jìn)行化學(xué)結(jié)構(gòu)的表征檢測(cè),凝膠電泳阻滯試驗(yàn)結(jié)合原子力顯微鏡觀察RGD-CS對(duì)質(zhì)粒的包裹情況及RGD-CS/pDNA復(fù)合體的形態(tài),EB染色法檢測(cè)鈦片表面RGD-CS/pDNA復(fù)合體的接枝效果。結(jié)果紅外光譜檢測(cè)結(jié)合元素分析表明,CS和RGD肽偶聯(lián)成功;凝膠電泳阻滯試驗(yàn)結(jié)合原子力顯微鏡觀察表明,在N/P≥2時(shí),RGD-CS與pDNA完全復(fù)合,RGD-CS/pDNA復(fù)合體呈類球形;EB染色表明RGD-CS/pDNA復(fù)合體接枝鈦片成功。結(jié)論經(jīng)RGD肽修飾的殼聚糖可以攜帶pDNA作為鈦種植體表面質(zhì)粒包裝的載體。
[Abstract]:Objective to explore a new method to improve the surface treatment of titanium implantation and to improve the osteogenic efficiency after implantation. Methods RGD-CS/pDNA complex was prepared by coupling RGD peptide with chitosan (CS) by acylation to form RGD-CS/pDNA by complex coagulation method, and RGD-CS/pDNA complex was graft onto pure titanium surface after physical and biochemical treatment. The chemical structure of RGD-CS was characterized by infrared spectrometer and element analyzer. The encapsulation of plasmid by RGD-CS and the morphology of RGD-CS/pDNA complex were observed by gel electrophoresis block test combined with atomic force microscope. The grafting effect of RGD-CS/pDNA complex on titanium sheet was detected by EB staining. Results Infrared spectroscopy combined with elemental analysis showed that the coupling of CS and RGD peptides was successful, gel electrophoresis block test combined with atomic force microscope showed that when N 鈮，

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