【摘要】：背景:大量研究證實釉基質(zhì)蛋白可促進成骨細胞和成牙骨質(zhì)細胞的再生,將其運用于牙周缺損治療可達到接近生理性的牙周再生。目的:觀察不同質(zhì)量濃度釉基質(zhì)蛋白對人牙周膜細胞增生、分化和遷移的影響。方法:取第3代人牙周膜細胞,以含不同質(zhì)量濃度釉基質(zhì)蛋白(0,12.5,25,50,100,250 mg/L)的無血清DMEM培養(yǎng)基培養(yǎng)。培養(yǎng)24 h后,采用3H-胸腺嘧啶核苷摻入法檢測細胞增殖,MTT法檢測細胞活性;培養(yǎng)48 h后,檢測細胞堿性磷酸酶活性及骨鈣素分泌;待細胞融合為單層,去除細胞培養(yǎng)液,以移液管頭將單層細胞制備出1 mm寬的細胞切口,持續(xù)24 h觀察細胞融合情況。結果與結論:當釉基質(zhì)蛋白質(zhì)量濃度在0-100 mg/L范圍內(nèi),隨著其質(zhì)量濃度的升高,細胞增殖、活性、堿性磷酸酶活性、骨鈣素分泌均逐漸升高,以100 mg/L升高最明顯;當釉基質(zhì)蛋白質(zhì)量濃度增至250 mg/L時,細胞增殖、活性、堿性磷酸酶活性、骨鈣素分泌均有所下降,但仍高于0 mg/L組。100 mg/L組在初始觀察6 h時,創(chuàng)緣周圍的細胞開始向中心生長,待培養(yǎng)12 h時,創(chuàng)緣兩側細胞開始融合,培養(yǎng)20 h后創(chuàng)緣兩側細胞融合完全創(chuàng)緣完全關閉完全,創(chuàng)面愈合優(yōu)于其他質(zhì)量濃度組。結果表明釉基質(zhì)蛋白具有促進牙周膜細胞增殖、分化與遷移的能力。
[Abstract]:Background: a large number of studies have confirmed that ameloblast can promote the regeneration of osteoblasts and osteoblasts, and its application in the treatment of periodontal defects can achieve near physiological periodontal regeneration. Aim: to observe the effects of different concentrations of ameloblast on proliferation, differentiation and migration of human periodontal ligament cells. Methods: the third generation of human periodontal ligament cells were cultured in serum-free DMEM medium containing different concentrations of amellae matrix protein (0, 12.5, 25, 50100250 mg/L). After 24 hours of culture, cell proliferation was detected by 3H-thymidine incorporation assay, cell activity was detected by MTT assay, alkaline phosphatase activity and osteocalcin secretion were detected after 48 hours of culture, cell fusion into monolayer was removed, 1 mm wide cell incision was prepared with liquid transfer tube head, and cell fusion was observed for 24 hours. Results and conclusion: when the concentration of amel matrix protein was in the range of 0 鈮，

本文編號：2505950