【摘要】：背景:大量研究證實(shí)釉基質(zhì)蛋白可促進(jìn)成骨細(xì)胞和成牙骨質(zhì)細(xì)胞的再生,將其運(yùn)用于牙周缺損治療可達(dá)到接近生理性的牙周再生。目的:觀察不同質(zhì)量濃度釉基質(zhì)蛋白對(duì)人牙周膜細(xì)胞增生、分化和遷移的影響。方法:取第3代人牙周膜細(xì)胞,以含不同質(zhì)量濃度釉基質(zhì)蛋白(0,12.5,25,50,100,250 mg/L)的無(wú)血清DMEM培養(yǎng)基培養(yǎng)。培養(yǎng)24 h后,采用3H-胸腺嘧啶核苷摻入法檢測(cè)細(xì)胞增殖,MTT法檢測(cè)細(xì)胞活性;培養(yǎng)48 h后,檢測(cè)細(xì)胞堿性磷酸酶活性及骨鈣素分泌;待細(xì)胞融合為單層,去除細(xì)胞培養(yǎng)液,以移液管頭將單層細(xì)胞制備出1 mm寬的細(xì)胞切口,持續(xù)24 h觀察細(xì)胞融合情況。結(jié)果與結(jié)論:當(dāng)釉基質(zhì)蛋白質(zhì)量濃度在0-100 mg/L范圍內(nèi),隨著其質(zhì)量濃度的升高,細(xì)胞增殖、活性、堿性磷酸酶活性、骨鈣素分泌均逐漸升高,以100 mg/L升高最明顯;當(dāng)釉基質(zhì)蛋白質(zhì)量濃度增至250 mg/L時(shí),細(xì)胞增殖、活性、堿性磷酸酶活性、骨鈣素分泌均有所下降,但仍高于0 mg/L組。100 mg/L組在初始觀察6 h時(shí),創(chuàng)緣周?chē)募?xì)胞開(kāi)始向中心生長(zhǎng),待培養(yǎng)12 h時(shí),創(chuàng)緣兩側(cè)細(xì)胞開(kāi)始融合,培養(yǎng)20 h后創(chuàng)緣兩側(cè)細(xì)胞融合完全創(chuàng)緣完全關(guān)閉完全,創(chuàng)面愈合優(yōu)于其他質(zhì)量濃度組。結(jié)果表明釉基質(zhì)蛋白具有促進(jìn)牙周膜細(xì)胞增殖、分化與遷移的能力。
[Abstract]:Background: a large number of studies have confirmed that ameloblast can promote the regeneration of osteoblasts and osteoblasts, and its application in the treatment of periodontal defects can achieve near physiological periodontal regeneration. Aim: to observe the effects of different concentrations of ameloblast on proliferation, differentiation and migration of human periodontal ligament cells. Methods: the third generation of human periodontal ligament cells were cultured in serum-free DMEM medium containing different concentrations of amellae matrix protein (0, 12.5, 25, 50100250 mg/L). After 24 hours of culture, cell proliferation was detected by 3H-thymidine incorporation assay, cell activity was detected by MTT assay, alkaline phosphatase activity and osteocalcin secretion were detected after 48 hours of culture, cell fusion into monolayer was removed, 1 mm wide cell incision was prepared with liquid transfer tube head, and cell fusion was observed for 24 hours. Results and conclusion: when the concentration of amel matrix protein was in the range of 0 鈮，

本文編號(hào)：2505950