發(fā)布時(shí)間：2019-06-15 12:17

【摘要】：目的:實(shí)驗(yàn)一研究3.5代羧基改性后的聚酰胺-胺樹(shù)枝狀高分子聚合物及其誘導(dǎo)人類脫礦牙本質(zhì)仿生再礦化作用。不同濃度的PAMAM溶液誘導(dǎo)脫礦牙本質(zhì)仿生再礦化的能力是否有差異為本實(shí)驗(yàn)的主要研究側(cè)重點(diǎn)。實(shí)驗(yàn)二合成并表征同時(shí)具有有機(jī)、無(wú)機(jī)材料特性的納米復(fù)合生物材料聚酰胺-胺/納米羥磷灰石(PAMAM/n-HAP),并觀察其對(duì)牙本質(zhì)小管的封閉作用。方法:實(shí)驗(yàn)一通過(guò)口腔頜面外科收集40顆完整離體第三磨牙,并將其相應(yīng)制備成脫礦牙本質(zhì)模型(每顆牙齒制備一個(gè)模型)。隨后隨機(jī)分為A、B、C、D4組,分別接受不同濃度3.5代羧基改性的聚酰胺-胺聚合物(PAMAM-COOH)處理,后浸泡于人工唾液接受再礦化處理14d,后使用傅里葉紅外變換光譜(FTIR)檢測(cè)PAMAM-COOH分子與牙本質(zhì)表面的結(jié)合情況;牙本質(zhì)小管的微觀形貌通過(guò)掃描電子顯微鏡(SEM)表征。實(shí)驗(yàn)二通過(guò)實(shí)驗(yàn)室制備PAMAM/n-HAP納米復(fù)合生物材料(采用Pramanik水化學(xué)反應(yīng)的方法)。使用透射電子顯微鏡(TEM)、傅里葉變換紅外光譜儀(FTIR)對(duì)納米復(fù)合生物材料進(jìn)行表征和驗(yàn)證。將脫礦牙本質(zhì)模型隨機(jī)分為A、B、C、D四組,分別采用等量的去離子水、PAMAM/n-HAP、n-HAP、PAMAM/n-HAP處理,A、B、C三組浸泡于人工唾液7d接受再礦化處理,D組浸泡于去離子水中7d。使用掃描電子顯微鏡(SEM)對(duì)其牙本質(zhì)小管的修復(fù)封閉效果進(jìn)行檢測(cè)。并采用顯微硬度儀對(duì)其機(jī)械性能的恢復(fù)進(jìn)行檢測(cè)和比較。結(jié)果:實(shí)驗(yàn)一:FTIR結(jié)果顯示G3.5 PAMAM-COOH能夠牢固結(jié)合于脫礦牙本質(zhì)模型的表面,并且高濃度PAMAM-COOH處理后的牙本質(zhì)磨片顯示出更強(qiáng)的特征峰值。SEM結(jié)果顯示應(yīng)用不同濃度的PAMAM-COOH雖然能夠在一定程度誘導(dǎo)新生的礦物晶體沉積封閉牙本質(zhì)小管,然而均不能夠高效的封閉暴露的牙本質(zhì)小管。SEM的結(jié)果顯示,不同濃度PAMAM-COOH所誘導(dǎo)的實(shí)驗(yàn)組未見(jiàn)明顯的封閉差異。實(shí)驗(yàn)二:FTIR顯示1244 cm-1和1650 cm-1出現(xiàn)了PAMAM所特有的強(qiáng)振動(dòng)峰,同時(shí)TEM顯示納米羥磷灰石的表面出現(xiàn)10nm厚的低襯度包裹層,證實(shí)了PAMAM/n-HAP的成功合成。SEM顯示應(yīng)用PAMAM/n-HAP能夠有效的降低牙本質(zhì)小管的直徑,修復(fù)并填充空虛的牙本質(zhì)小管,封閉牙本質(zhì)小管,從而獲得良好的封閉作用。結(jié)論:1.G3.5 PAMAM-COOH能夠吸附并穩(wěn)定于納米羥磷灰石的表面。2.在某種程度上,G3.5 PAMAM-COOH能夠誘導(dǎo)礦化沉積物沉積于牙本質(zhì)磨片表面或者小管內(nèi)部,但是晶體外形不規(guī)則,礦化沉積物量較少,封閉效果不理想,并且本實(shí)驗(yàn)的掃描電鏡結(jié)果顯示不同濃度的G3.5PAMAM-COOH(1000ppm,5000ppm,以及10000ppm)其誘導(dǎo)牙本質(zhì)小管封閉的效果并沒(méi)有明顯的差異。3.作為一種新型的納米復(fù)合生物材料,人工合成的PAMAM/n-HAP具有良好的生物活性,能夠有效地封閉牙本質(zhì)小管,同時(shí)有較為理想的封閉深度,提示PAMAM/n-HAP可能成為一種新型的脫敏材料或者脫敏藥物添加劑應(yīng)用于臨床。4.PAMAM/n-HAP通過(guò)修復(fù)脫礦牙本質(zhì)小管,能夠恢復(fù)其一定的硬度,說(shuō)明其在一定程度能夠恢復(fù)機(jī)械性能。
[Abstract]:Objective: To study the biomimetic re-mineralization of polyetheramine-amine dendrimer and its induced demineralization of human demineralization. The ability of the different concentration of the PAMAM solution to induce the biomimetic re-mineralization of the demineralization dentin is different from the main research focus of the experiment. The composite biological materials with organic and inorganic materials (PAMAM/ n-HAP) were synthesized and characterized at the same time, and its effect on the dentinal tubules was observed. Methods:40 intact third molars were collected by oral and maxillofacial surgery and prepared into a demineralization dentin model (a model for each tooth). then the groups are randomly divided into groups A, B, C and D4, and then treated by a polycarbodiimide-amine polymer (PAMAM-COOH) with different concentration of 3.5 generations, and then is soaked in artificial saliva to be subjected to the re-mineralization treatment 14d, The binding of PAMAM-COOH and the surface of dentin was detected by FTIR. The micromorphology of the dentinal tubules was characterized by scanning electron microscopy (SEM). The preparation of PAMAM/ n-HAP nano-composite biomaterial by the laboratory was carried out by a laboratory. The composite biological materials were characterized and verified by a transmission electron microscope (TEM) and a Fourier transform infrared spectrometer (FTIR). The demineralization dentin model was randomly divided into four groups: A, B, C and D, and the same amount of deionized water, PAMAM/ n-HAP, n-HAP, PAMAM/ n-HAP were used, and the three groups of A, B and C were soaked in artificial saliva for 7 days to be re-mineralized, and the D group was soaked in deionized water for 7 days. A scanning electron microscope (SEM) was used to test the effect of the repair and closure of the dentinal tubules. And the mechanical property recovery of the device is detected and compared by a micro-hardness meter. Results: The results showed that the results showed that the results of FTIR showed that the G3. 5PAMAM-COOH can be firmly combined with the surface of the demineralization dentine model, and the high-concentration PAMAM-COOH treated dentin grinding disc shows a stronger characteristic peak. SEM results show that the application of PAMAM-COOH at different concentrations can induce new mineral crystals to close the dentinal tubules to a certain extent, but can not close the exposed dentinal tubules efficiently. The results of SEM showed that there was no significant difference in the experimental group induced by different concentration of PAMAM-COOH. Experiment 2: FTIR showed that 1244 cm-1 and 1650 cm-1 showed a strong vibration peak unique to the PAMAM, while the TEM showed that the surface of the nano-hydroxyapatite was 10 nm thick low-contrast coating layer, which confirmed the successful synthesis of the PAMAM/ n-HAP. SEM showed that the application of PAMAM/ n-HAP can effectively reduce the diameter of the dentinal tubules, repair and fill the empty dentin tubules and close the dentinal tubules, thus obtaining a good sealing effect. Conclusion:1. G3. 5PAMAM-COOH can adsorb and stabilize the surface of the nano-hydroxyapatite. In some ways, the G3. 5PMAM-COOH can induce the deposit of the mineralized deposit on the surface of the dentin grinding disc or the inside of the tubule, but the crystal is irregular, the amount of the mineralized deposit is small, the sealing effect is not ideal, and the scanning electron microscope result of the experiment shows that the G3. 5PAMAM-COOH (1000 ppm,5000 ppm, And there was no significant difference in the effect of the induction of the closure of the dentinal tubules. As a novel nano composite biological material, the artificially synthesized PAMAM/ n-HAP has good biological activity, can effectively close the dentinal tubules, and has an ideal sealing depth, It is suggested that the PAMAM/ n-HAP may be a new desensitizing material or desensitizing drug additive.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R781.2
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本文編號(hào)：2500201