發(fā)布時間：2019-06-05 06:04

【摘要】：目的:口腔扁平苔蘚（oral lichen planus，，OLP），是口腔黏膜的慢性非感染性疾病，是一種較為常見的口腔黏膜疾病，其發(fā)病僅低于復(fù)發(fā)性口腔潰瘍，病情反復(fù)發(fā)作，可伴充血糜爛，難以治愈，屬于癌前狀態(tài)。其病因尚不明確，有研究顯示，OLP是一種由T淋巴細(xì)胞介導(dǎo)的自身免疫反應(yīng)性疾病。 本研究通過檢測腫瘤壞死因子樣配體1A（tumor necrosis factor-like ligand1A，TL1A）和吲哚胺2，3雙加氧酶（indoleamine2，3-dioxygenase，IDO）在健康人、口腔扁平苔蘚患者外周血單個核細(xì)胞中的表達(dá)水平，探討兩者分別在口腔扁平苔蘚中的表達(dá)及意義，推測這兩個因子在扁平苔蘚發(fā)病機制中的作用。 方法:采用實時熒光定量聚合酶鏈?zhǔn)椒磻?yīng)（Real-time fluorescent quantitative PCR，F(xiàn)Q-PCR）法檢測30例OLP患者和30例健康者外周血單個核細(xì)胞（peripheral bloodmononuclear cell，PBMCs）中TL1A、IDO mRNA的相對表達(dá)量，選β肌動蛋白（β-actin）為內(nèi)參照基因，F(xiàn)Q-PCR中采用2-△△CT方法，檢測TL1A、IDO mRNA的相對表達(dá)量。 結(jié)果:1.在口腔扁平苔蘚患者外周血單個核細(xì)胞中，TL1A的基因表達(dá)水平均高于健康對照組，經(jīng)統(tǒng)計學(xué)分析，有顯著性差異(P 0.05)�？谇槐馄教μ\患者組中TL1A的基因表達(dá)量是正常對照組的1.7872倍。 2．在口腔扁平苔蘚患者外周血單個核細(xì)胞中，IDO的基因表達(dá)水平均高于健康對照組，經(jīng)統(tǒng)計學(xué)分析，有顯著性差異(P 0.05)�？谇槐馄教μ\患者組IDO的基因表達(dá)水平是正常對照組的2.0763倍。 3.在口腔扁平苔蘚患者組，TL1A和IDO基因表達(dá)量經(jīng)Pearson線性相關(guān)分析，相關(guān)系數(shù)r=0.124，(P=0.5150.05)，表明兩組數(shù)據(jù)無相關(guān)性。 結(jié)論:1. TL1AmRNA在OLP患者組中的表達(dá)水平明顯高于正常對照組，表明TL1A可能參與OLP的發(fā)病過程。 2. IDOmRNA在OLP患者組中的表達(dá)水平明顯高于正常對照組，表明IDO可能參與OLP炎癥的發(fā)生發(fā)展，其機制有待于進(jìn)一步研究。 3.在OLP患者組TL1A和IDO基因表達(dá)無直接相關(guān)性，TL1A和IDO也有可能通過間接途徑或方式發(fā)揮著作用。
[Abstract]:Objective: oral lichen planus (oral lichen planus,OLP) is a chronic non-infectious disease of oral mucous membrane. It is a common oral mucous membrane disease, which is only lower than that of recurrent oral ulcer and can be accompanied by hyperemia and erosion. Difficult to cure, belonging to the precancerous state. Its etiology is not clear, some studies have shown that OLP is an autoimmune disease mediated by T lymphocytes. In this study, the expression of tumor necrosis factor-like ligand 1A (tumor necrosis factor-like ligand1A,TL1A and indoleamine 2, 3 dioxygenase (indoleamine2,3-dioxygenase,IDO) in peripheral blood mononuclear cells of healthy people and patients with oral lichen planus was detected. To explore the expression and significance of the two factors in oral lichen planus, and to speculate the role of these two factors in the pathogenesis of oral lichen planus. Methods: the relative expression of TL1A,IDO mRNA in peripheral blood mononuclear cells (peripheral bloodmononuclear cell,PBMCs) of 30 patients with OLP and 30 healthy controls was detected by real-time fluorescence quantitative polymerase chain reaction (Real-time fluorescent quantitative PCR,FQ-PCR). 尾-actin (尾-actin) was selected as internal reference gene. 2-CT method was used to detect the relative expression of TL1A,IDO mRNA in FQ-PCR. Result: 1. The gene expression level of TL1A in peripheral blood mononuclear cells of patients with oral lichen planus was higher than that of healthy control group, and there was significant difference between the two groups (P 0.05). The expression of TL1A gene in oral lichen planus group was 1.7872 times higher than that in normal control group. 2. The gene expression level of IDO in peripheral blood mononuclear cells of patients with oral lichen planus was higher than that of healthy control group, and there was significant difference between the two groups (P 0.05). The gene expression level of IDO in oral lichen planus group was 2.0763 times higher than that in normal control group. 3. In the oral lichen planus group, the expression of TL1A and IDO genes was analyzed by Pearson linear correlation analysis, and the correlation coefficient was r 鈮

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