Beyond祛氟劑和唾液再礦化對(duì)恒牙釉質(zhì)影響的實(shí)驗(yàn)研究
發(fā)布時(shí)間：2019-05-21 23:51
【摘要】：目的： 通過(guò)冷光美白聯(lián)合祛氟劑及唾液處理恒牙釉質(zhì)表面物理性能和形態(tài)結(jié)構(gòu)的觀察分析，研究健康恒牙表層釉質(zhì)顯微硬度、成分、結(jié)構(gòu)受祛氟劑和唾液再礦化的影響，為臨床應(yīng)用祛氟劑提供理論依據(jù)。 實(shí)驗(yàn)方法： 選擇近期因需正畸治療而拔除的健康上頜前磨牙30顆，沿近遠(yuǎn)中向和頰舌向切割牙冠成4部分，得到120個(gè)樣本。選擇測(cè)試面平行于水平面的100個(gè)樣本，隨機(jī)分成10組�？瞻讓�(duì)照組，Beyond美白凝膠組（即刻組，一周組，兩周組），Beyond美白凝膠+脫色劑（Ｉ液）（即刻組，一周組，兩周組），Beyond美白凝膠+脫色劑（Ｉ液）和再礦化液（II液）（即刻組，一周組，兩周組），每組10個(gè)樣本。 其中空白組和各個(gè)即刻組，實(shí)驗(yàn)即刻觀察；各一周組實(shí)驗(yàn)浸泡在唾液中置于37度恒溫水浴箱內(nèi)一周后觀察，各兩周組為實(shí)驗(yàn)浸泡在唾液中置于37度恒溫水浴箱內(nèi)兩周后觀察。全部樣本使用掃描電子顯微鏡觀測(cè)表面形態(tài)，結(jié)合能譜分析儀測(cè)定牙釉質(zhì)表面的鈣、磷元素含量及鈣/磷比值，牙釉質(zhì)表面使用顯微硬度儀測(cè)定顯微硬度，，所得數(shù)據(jù)通過(guò)SPSS19.0軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果： 1、掃描電鏡觀測(cè)，低倍鏡觀察空白組釉質(zhì)可見(jiàn)正常釉質(zhì)形態(tài)結(jié)構(gòu)，表面較為光滑，高倍鏡觀察釉柱排列呈魚(yú)鱗狀外貌。 實(shí)驗(yàn)即刻：Beyond組低倍鏡觀察釉質(zhì)表面表面粗糙，高倍鏡觀察出現(xiàn)明顯酸蝕改變，孔隙率增加，釉柱中心及其周?chē)梢?jiàn)明顯脫礦溶解，呈現(xiàn)深陷鏤空的蟻穴狀結(jié)構(gòu)。Beyond+I液組低倍鏡觀察牙釉質(zhì)表面表面粗糙比Beyond即刻組嚴(yán)重，高倍鏡觀察酸蝕明顯，正常的釉質(zhì)表面結(jié)構(gòu)已經(jīng)消失，代替為明顯的空隙和表面凹陷，釉柱中心不明顯，呈現(xiàn)密集的空洞表面。低倍鏡觀察，Beyond+III液組牙釉質(zhì)粗糙度優(yōu)于Beyond組，高倍鏡觀察能夠看到空隙率增加，可見(jiàn)到微孔和微融的空洞狀釉質(zhì)表面。 浸泡一周后：低倍鏡下觀察Beyond組、Beyond+I液組、Beyond+III液組釉質(zhì)表面粗糙程度減輕，高倍鏡觀察均有礦物質(zhì)沉積，牙釉質(zhì)釉柱中心及其周?chē)梢?jiàn)到部分再礦化，表面空隙率減少，空洞結(jié)構(gòu)密集度降低。Beyond+I液組孔隙結(jié)構(gòu)較Beyond組多，Beyond+III液組孔隙率較低。 浸泡兩周后：低倍鏡下觀察低倍鏡下Beyond組、Beyond+I液組、Beyond+III液組釉質(zhì)表面均較為平坦，礦物質(zhì)沉積明顯，基本恢復(fù)釉質(zhì)正常形態(tài)。Beyond+I液組組高倍鏡觀察牙釉質(zhì)表面仍可見(jiàn)到部分孔隙結(jié)構(gòu)，但數(shù)目明顯少于即刻組。Beyond組和Beyond+III液組較少看到孔隙結(jié)構(gòu)。 2、能譜分析結(jié)果，即刻組中：Beyond、Beyond+I液顯微硬度比值較空白對(duì)照組有顯著性差異，p0.05，；Beyond+I液與Beyond+III液有顯著性差異，p0.05，具有統(tǒng)計(jì)學(xué)意義。其余組間差異不具有統(tǒng)計(jì)學(xué)意義。 3、即刻組中：Beyond、Beyond+I液顯微硬度比值較空白對(duì)照組有顯著性差異，p0.05；Beyond、Beyond+III液顯微硬度比值較Beyond+I組有顯著性差異，p0.05，具有統(tǒng)計(jì)學(xué)意義。其余組間差異不具有統(tǒng)計(jì)學(xué)意義。 結(jié)論： 1、冷光美白治療單獨(dú)使用脫色劑，會(huì)加重單純使用冷光美白劑對(duì)釉質(zhì)的腐蝕，釉質(zhì)表面粗糙加重，呈現(xiàn)明顯的脫礦改變。 2、冷光美白使用脫色劑聯(lián)合再礦化液，處理后的牙釉質(zhì)表面物理特性較為穩(wěn)定，顯微硬度無(wú)顯著性變化。單純應(yīng)用冷光美白劑進(jìn)行臨床治療后，對(duì)牙齒進(jìn)行再礦化治療，可加速美白后釉質(zhì)表面鈣磷沉積。 3、唾液具有再礦化功能，唾液浸泡保存可使因美白而致的脫礦釉質(zhì)表面礦物質(zhì)含量出現(xiàn)上升趨勢(shì)，可修復(fù)因美白所致的釉質(zhì)表面脫礦。
[Abstract]:Purpose: Through the observation and analysis of the physical properties and the morphological structure of the surface of the permanent enamel treated with the combination of the cold-light whitening and the defluorinating agent and the saliva, the micro-hardness, the composition and the structure of the surface enamel of the healthy permanent tooth were studied, and the shadow of the defluorinating agent and the salivation of the saliva were studied. In response, a theory is provide for that clinical application of a fluorine-removing agent. According to. Real Methods:30 healthy maxillary premolars extracted from the recent orthodontic treatment were selected, and the crown was cut into 4 parts along the proximal and buccal tongue, and 1 was obtained. 20 samples. Select 100 samples of the test plane parallel to the horizontal plane, random divided into 10 groups: blank control group, Beyond whitening gel group (immediate group, one-week group, two-week group), Beyond whitening gel + decolorizer (I-liquid) (immediate group, one-week group, two-week group), Beyond whitening gel + decolorizer (I-liquid) and re-mineralized liquid (II-liquid) (immediate group, one-week group), , two-week group), each group 10 samples. In the blank group and in the immediate group, the experiment was immediately observed; each week group experiment was soaked in saliva to be placed in a 37-degree constant-temperature water bath for one week, and the two-week group was immersed in saliva in a constant-temperature water bath of 37 degrees. After two weeks in the box, the surface morphology was observed with a scanning electron microscope. The calcium, phosphorus content and calcium/ phosphorus ratio of the surface of the enamel were measured by means of the energy spectrum analyzer. The surface of the enamel was measured by a micro-hardness instrument. The obtained data was obtained by the SPSS19.0 software. to be in series The results were as follows:1, the observation of the scanning electron microscope, the observation of the normal enamel structure of the blank group in the blank group, the smooth surface of the surface and the view of high power The results showed that the surface of the enamel surface was rough and the porosity increased, the center of the enamel column and the surrounding of the enamel column were obviously demined and dissolved. In Beyond + I group, the surface roughness of enamel surface is more severe than that of the immediate group of Beyond, and the high-power mirror has obvious acid corrosion. The normal enamel surface structure has disappeared, instead of the obvious void and surface depression, the center of the glaze column It is not obvious that the dense cavity surface is present. The low-magnification observation shows that the enamel roughness of the Beyond + III liquid group is better than that of the Beyond group, and the high-power mirror observation can see the increase of the void ratio, which can be seen. After a week, the surface roughness of the enamel surface of the Beyond group, the Beyond + I liquid group and the Beyond + III liquid group was reduced, and the surface roughness of the enamel surface of the Beyond + I liquid group and the Beyond + III liquid group was reduced. The void ratio is reduced, and the density of the hollow structure is reduced. The pore structure of the Beyond + I liquid group is more than that of the Beyond group, Be The porosity of the yond + III liquid group was lower. After two weeks of soaking, the low-fold microscopic observation of the second group, the Beyond + I liquid group, the Beyond + III liquid group and the enamel surface of the Beyond + III liquid group were relatively flat, In Beyond + I group, the surface of enamel can still be seen. Part of the pore structure, but the number is significantly less than the immediate group. The Beyond group and the Bey The results of spectrum analysis showed that the ratio of the microhardness of Beyond and Beyond + I in the immediate group was significantly different from that of the blank control group. significant difference, p0.05, with system The difference between the other groups was not statistically significant.3. The ratio of the microhardness of the Beyond and the Beyond + I fluid in the immediate group was significantly different from that of the blank control group, and the ratio of the microhardness of the Beyond + III solution to the Beyond + I was higher than that of the Beyond + I. There was a significant difference in the group, p0.05, Statistics The difference between the other groups was not statistically significant. Conclusion:1. The cold-light whitening treatment alone uses the decolorizer, which can increase the pure use of the cold-light beauty. the corrosion of the enamel, the surface roughness of the enamel, the obvious demineralization change,2, the combination of the cold-light whitening and the destaining agent and the mineralizing liquid, The physical properties of the posterior enamel surface were stable and the microhardness was not significantly changed. After the tooth is re-mineralized and treated, the calcium and phosphorus deposition on the surface of the enamel after whitening can be accelerated.3, the saliva has a remineralization function, and the saliva is soaked and stored, so that the demineralization enamel caused by the whitening can be obtained.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R783
本文編號(hào)：2482508
[Abstract]:Purpose: Through the observation and analysis of the physical properties and the morphological structure of the surface of the permanent enamel treated with the combination of the cold-light whitening and the defluorinating agent and the saliva, the micro-hardness, the composition and the structure of the surface enamel of the healthy permanent tooth were studied, and the shadow of the defluorinating agent and the salivation of the saliva were studied. In response, a theory is provide for that clinical application of a fluorine-removing agent. According to. Real Methods:30 healthy maxillary premolars extracted from the recent orthodontic treatment were selected, and the crown was cut into 4 parts along the proximal and buccal tongue, and 1 was obtained. 20 samples. Select 100 samples of the test plane parallel to the horizontal plane, random divided into 10 groups: blank control group, Beyond whitening gel group (immediate group, one-week group, two-week group), Beyond whitening gel + decolorizer (I-liquid) (immediate group, one-week group, two-week group), Beyond whitening gel + decolorizer (I-liquid) and re-mineralized liquid (II-liquid) (immediate group, one-week group), , two-week group), each group 10 samples. In the blank group and in the immediate group, the experiment was immediately observed; each week group experiment was soaked in saliva to be placed in a 37-degree constant-temperature water bath for one week, and the two-week group was immersed in saliva in a constant-temperature water bath of 37 degrees. After two weeks in the box, the surface morphology was observed with a scanning electron microscope. The calcium, phosphorus content and calcium/ phosphorus ratio of the surface of the enamel were measured by means of the energy spectrum analyzer. The surface of the enamel was measured by a micro-hardness instrument. The obtained data was obtained by the SPSS19.0 software. to be in series The results were as follows:1, the observation of the scanning electron microscope, the observation of the normal enamel structure of the blank group in the blank group, the smooth surface of the surface and the view of high power The results showed that the surface of the enamel surface was rough and the porosity increased, the center of the enamel column and the surrounding of the enamel column were obviously demined and dissolved. In Beyond + I group, the surface roughness of enamel surface is more severe than that of the immediate group of Beyond, and the high-power mirror has obvious acid corrosion. The normal enamel surface structure has disappeared, instead of the obvious void and surface depression, the center of the glaze column It is not obvious that the dense cavity surface is present. The low-magnification observation shows that the enamel roughness of the Beyond + III liquid group is better than that of the Beyond group, and the high-power mirror observation can see the increase of the void ratio, which can be seen. After a week, the surface roughness of the enamel surface of the Beyond group, the Beyond + I liquid group and the Beyond + III liquid group was reduced, and the surface roughness of the enamel surface of the Beyond + I liquid group and the Beyond + III liquid group was reduced. The void ratio is reduced, and the density of the hollow structure is reduced. The pore structure of the Beyond + I liquid group is more than that of the Beyond group, Be The porosity of the yond + III liquid group was lower. After two weeks of soaking, the low-fold microscopic observation of the second group, the Beyond + I liquid group, the Beyond + III liquid group and the enamel surface of the Beyond + III liquid group were relatively flat, In Beyond + I group, the surface of enamel can still be seen. Part of the pore structure, but the number is significantly less than the immediate group. The Beyond group and the Bey The results of spectrum analysis showed that the ratio of the microhardness of Beyond and Beyond + I in the immediate group was significantly different from that of the blank control group. significant difference, p0.05, with system The difference between the other groups was not statistically significant.3. The ratio of the microhardness of the Beyond and the Beyond + I fluid in the immediate group was significantly different from that of the blank control group, and the ratio of the microhardness of the Beyond + III solution to the Beyond + I was higher than that of the Beyond + I. There was a significant difference in the group, p0.05, Statistics The difference between the other groups was not statistically significant. Conclusion:1. The cold-light whitening treatment alone uses the decolorizer, which can increase the pure use of the cold-light beauty. the corrosion of the enamel, the surface roughness of the enamel, the obvious demineralization change,2, the combination of the cold-light whitening and the destaining agent and the mineralizing liquid, The physical properties of the posterior enamel surface were stable and the microhardness was not significantly changed. After the tooth is re-mineralized and treated, the calcium and phosphorus deposition on the surface of the enamel after whitening can be accelerated.3, the saliva has a remineralization function, and the saliva is soaked and stored, so that the demineralization enamel caused by the whitening can be obtained.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R783
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