【摘要】：【目的】 研究細(xì)胞癌變相關(guān)蛋白B淋巴細(xì)胞瘤-2基因（Bcl-2）、泛素蛋白連接酶MDM2、細(xì)胞周期依賴性激酶抑制因子P21在人口腔正常黏膜、白斑、鱗狀細(xì)胞癌細(xì)胞中的表達(dá)變化，進一步深入研究口腔鱗癌的發(fā)生發(fā)展機制提供科學(xué)依據(jù)。 【方法】 采用免疫組化二步法檢測正常15例正�？谇火つ�、25例口腔黏膜白斑、25例口腔鱗癌細(xì)胞中Bcl-2、MDM2和P21蛋白的表達(dá)情況，并通過免疫組化圖像定量軟件進行表達(dá)的相對定量分析，以統(tǒng)計軟件對實驗結(jié)果進行統(tǒng)計學(xué)分析。 【結(jié)果】 1、Bcl-2、MDM2蛋白在人口腔正常黏膜、單純增生性白斑、異常增生性白斑和口腔鱗癌細(xì)胞中的表達(dá)逐漸遞增（P＜0.05）。 2、P21蛋白在口腔正常黏膜、口腔單純增生性白斑細(xì)胞中呈陽性表達(dá)，在口腔異常增生白斑、口腔鱗狀細(xì)胞癌細(xì)胞中呈低水平表達(dá)。經(jīng)統(tǒng)計分析表明口腔異常增生白斑與口腔鱗狀細(xì)胞癌中P21蛋白表達(dá)差異具有統(tǒng)計學(xué)意義。 3、利用統(tǒng)計分析表明，在人異常增生性白斑和口腔鱗狀細(xì)胞癌組織中Bcl-2和MDM2蛋白表達(dá)呈正相關(guān)，而P21與Bcl-2和MDM2蛋白的表達(dá)未見顯著相關(guān)性。 【結(jié)論】 1、Bcl-2、MDM2蛋白在口腔異常白斑和鱗癌中的表達(dá)水平較口腔正常黏膜和單純增生性白斑中的表達(dá)明顯增高，而P21蛋白在異常鱗癌中的表達(dá)水平較口腔正常黏膜和單純白斑降低，提示Bcl-2、MDM2和P21蛋白表達(dá)異常與口腔鱗癌的的發(fā)生有關(guān)，因此可將Bcl-2、MDM2和P21蛋白作為口腔黏膜病變進展的監(jiān)測指標(biāo)，以提高早期口腔癌的發(fā)現(xiàn)率。 2、在口腔異常增生性白斑和口腔鱗癌細(xì)胞組織中，Bcl-2蛋白和MDM2蛋白的表達(dá)呈顯著正相關(guān)性，提示二者在口腔黏膜癌變中可能有協(xié)同作用。 3、雖然Bcl-2、MDM2和P21蛋白三者均是P53通路上P53基因的相關(guān)基因，但經(jīng)統(tǒng)計學(xué)分析，，未見P21蛋白的表達(dá)與Bcl-2蛋白或MDM2蛋白有相關(guān)性，提示P21蛋白對細(xì)胞癌變的作用機制不同于二者。
[Abstract]:[objective] to investigate the expression of cell carcinogenesis associated protein B lymphocyte tumor-2 gene (Bcl-2) and ubiquitin ligase MDM2, cell cycle-dependent kinase inhibitor P21 in human normal mucosa and leukoplakia. The changes of expression in squamous cell carcinoma provide scientific basis for further study on the pathogenesis of oral squamous cell carcinoma. [methods] the expression of Bcl-2,MDM2 and P21 protein in 15 cases of normal oral mucosa, 25 cases of oral leukoplakia and 25 cases of oral squamous cell carcinoma were detected by immunohistochemical two-step method. The relative quantitative analysis of the expression was carried out by the image quantitative software of immunohistochemistry, and the experimental results were analyzed statistically with the statistical software. [results] 1. The expression of Bcl-2, MDM2 protein increased gradually in normal mucosa, simple proliferative leukoplakia, dysplasia leukoplakia and oral squamous cell carcinoma (OSCC) (P < 0.05). 2. The expression of P21 protein was positive in normal oral mucosa, simple proliferative leukoplakia cells in oral cavity, and low expression in oral dysplasia leukoplakia and oral squamous cell carcinoma cells. Statistical analysis showed that there was a significant difference in the expression of P21 protein between oral dysplasia leukoplakia and oral squamous cell carcinoma. 3. Statistical analysis showed that there was a positive correlation between the expression of Bcl-2 and MDM2 protein in human dysplasia leukoplakia and oral squamous cell carcinoma, but there was no significant correlation between P21 and Bcl-2 and MDM2 protein expression. [conclusion] 1. The expression level of Bcl-2, MDM2 protein in oral abnormal leukoplakia and squamous cell carcinoma was significantly higher than that in normal oral mucosa and simple proliferative leukoplakia. The expression level of P21 protein in abnormal squamous cell carcinoma was lower than that in normal oral mucosa and simple leukoplakia, suggesting that the abnormal expression of Bcl-2,MDM2 and P21 protein was related to the occurrence of oral squamous cell carcinoma. Therefore, Bcl-2, could be expressed in oral squamous cell carcinoma. MDM2 and P21 proteins were used as indicators to monitor the progression of oral mucosal lesions in order to improve the detection rate of early oral cancer. 2. There was a significant positive correlation between the expression of Bcl-2 protein and MDM2 protein in dysplasia leukoplakia and oral squamous cell carcinoma, suggesting that they might play a synergistic role in the carcinogenesis of oral mucosa. 3. Although both Bcl-2,MDM2 and P21 proteins were related genes of p53 gene in p53 pathway, there was no correlation between P21 protein expression and Bcl-2 protein or MDM2 protein by statistical analysis, but there was no correlation between P21 protein expression and Bcl-2 protein or MDM2 protein. These results suggest that the mechanism of P21 protein on cell carcinogenesis is different from that of P21 protein.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R739.8

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