【摘要】：目的:通過(guò)建立大鼠正畸牙移動(dòng)后保持階段的實(shí)驗(yàn)?zāi)Ｐ?模仿臨床上正畸患者牙移動(dòng)后的保持,在實(shí)驗(yàn)動(dòng)物口腔內(nèi)局部注射藥物淫羊藿,觀察牙周組織中的BMP-2的表達(dá)變化,評(píng)價(jià)中藥淫羊藿在牙齒矯治之后抑制其復(fù)發(fā)方面的作用,探究淫羊藿在促進(jìn)牙周組織改建過(guò)程中的作用機(jī)理,為臨床中正畸治療后減短保持階段的時(shí)間以及使牙齒盡快的穩(wěn)定在理想的位置上提供了一種藥物輔助措施。方法:40只wistar大鼠分為實(shí)驗(yàn)組A組、B組C組和對(duì)照組,每組10只大鼠。在牙移動(dòng)模型去除之前的1d,實(shí)驗(yàn)組分別注射濃度為5ug/ml、10ug/ml、15ug/ml,的淫羊藿溶液,對(duì)照組同時(shí)注射同濃度,同劑量的生理鹽水,實(shí)驗(yàn)組和對(duì)照組都是每過(guò)2d注射一次,各組大鼠在裝置去除之后的第七天分別處死5只,第二十八天再分別處死5只,通過(guò)測(cè)量上頜第一磨牙的復(fù)發(fā)距離,HE染色觀測(cè)法以及免疫組織化學(xué)染色的方法,分析淫羊藿對(duì)大鼠牙移動(dòng)后保持階段牙周組織的改建和BMP-2的表達(dá)影響。結(jié)果:裝置去除后第7天,對(duì)照組的復(fù)發(fā)距離大于實(shí)驗(yàn)組各組復(fù)發(fā)距離分別為0.130mm、0.148mm、0.107mm。實(shí)驗(yàn)組成骨細(xì)胞數(shù)量比對(duì)照組分別增加0.32、0.44、0.21,實(shí)驗(yàn)組BMP-2表達(dá)平均光密度值比對(duì)照組分別增加0.019、0.023、0.014,實(shí)驗(yàn)組與對(duì)照組比較有顯著差異(P0.05)。裝置去除后第28天,對(duì)照組的復(fù)發(fā)距離大于實(shí)驗(yàn)組復(fù)發(fā)距離分別為,0.105mm、0.151mm、0.077mm。實(shí)驗(yàn)組成骨細(xì)胞數(shù)量比對(duì)照組分別增加0.38、0.63、0.01,實(shí)驗(yàn)組BMP-2表達(dá)平均光密度值比對(duì)照組分別增加0.030、0.045、0.023,實(shí)驗(yàn)組和對(duì)照組兩組比較有顯著差異(P0.05)。結(jié)論:局部注射中藥淫羊藿可以增加大鼠實(shí)驗(yàn)性牙移動(dòng)后保持階段中牙周組織B MP-2的表達(dá),對(duì)骨細(xì)胞的生成有促進(jìn)作用。
[Abstract]:Objective: to establish an experimental model of rat orthodontic tooth retention after tooth movement, and to observe the expression of BMP-2 in periodontal tissues by injecting epimedium into the oral cavity of experimental animals, mimicking the retention of orthodontic teeth after tooth movement. To evaluate the role of Herba Epimedii in inhibiting the recurrence of Epimedium after tooth treatment, and to explore the mechanism of Epimedium in promoting periodontal tissue remodeling. It provides a drug assistant measure for reducing the duration of maintenance after orthodontic treatment and keeping teeth stable in ideal position as soon as possible. Methods: 40 wistar rats were divided into three groups: experimental group A, group B, group C and control group, with 10 rats in each group. One day before the tooth movement model was removed, the experimental group was injected with epimedium solution with a concentration of 5 g / ml 10 g / ml and 15 g / ml, respectively, while the control group was injected with the same concentration and same dose of normal saline at the same time. The experimental group and the control group were injected every 2 days. The rats in each group were killed on the seventh day after the device was removed, and 5 rats were killed on the 28 day. The recurrence distance of the maxillary first molar was measured by measuring the recurrence distance of the maxillary first molar. The effects of Epimedium Epimedii on periodontal remodeling and expression of BMP-2 in rats after tooth movement were analyzed by HE staining and immunohistochemical staining. Results: on the 7th day after removal of the device, the recurrence distance of the control group was 0.130 mm / 0.148 mm / 0.107 mm, respectively. Compared with the control group, the number of osteoblasts in the experimental group increased by 0.32, 0.44 and 0.21, and the average optical density of BMP-2 expression in the experimental group increased by 0.019, 0.023 and 0.014, respectively. There was a significant difference between the experimental group and the control group (P0.05). On the 28th day after removal of the device, the recurrence distance of the control group was greater than that of the experimental group, 0.105mm / 0.151mm / 0.077mm. Compared with the control group, the number of osteoblasts in the experimental group increased by 0.38 ~ 0.63% and the average optical density of BMP-2 expression in the experimental group was 0.030 ~ 0.045 ~ 0.023, respectively. There was a significant difference between the experimental group and the control group (P0.05). Conclusion: local injection of Herba Epimedii can increase the expression of B MP-2 in periodontal tissue and promote the formation of osteocytes during the period of experimental tooth movement in rats.
【學(xué)位授予單位】：佳木斯大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R783.5

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 林建昌;賴文莉;;3種常用正畸保持器的特點(diǎn)及臨床應(yīng)用[J];國(guó)際口腔醫(yī)學(xué)雜志;2015年04期

2 李光輝;潘曉崗;;參與正畸牙移動(dòng)的相關(guān)因子研究進(jìn)展[J];口腔醫(yī)學(xué);2015年06期

3 陳林;烏蘭其其格;李宜宸;李碩光;張佳慧;趙哲;;三種正畸保持器的對(duì)比研究及進(jìn)展[J];內(nèi)蒙古醫(yī)科大學(xué)學(xué)報(bào);2015年S1期

4 陳雅竹;管曉燕;肖茜文;王斯瑋;劉建國(guó);;中草藥在正畸牙移動(dòng)牙周組織改建中的作用[J];中國(guó)組織工程研究;2014年46期

5 顧羊林;朱國(guó)興;;兔髖臼軟骨損傷后髖關(guān)節(jié)發(fā)育及髖臼軟骨中的骨形態(tài)發(fā)生蛋白7[J];中國(guó)組織工程研究;2014年33期

6 劉凱杰;李超;;淫羊藿藥理研究進(jìn)展(綜述)[J];亞熱帶植物科學(xué);2014年02期

7 丁曉華;莊金良;姜雨君;徐山山;陳遠(yuǎn)萍;;辛伐他汀對(duì)大鼠正畸牙保持階段牙周組織中BMP-2表達(dá)的影響[J];口腔醫(yī)學(xué)研究;2014年04期

8 蘇達(dá)明;趙軍華;黃志遠(yuǎn);李大廉;劉建軍;王敏;李華;;健膝強(qiáng)骨丸對(duì)膝骨關(guān)節(jié)炎家兔模型關(guān)節(jié)軟骨病理改變的影響[J];中國(guó)組織工程研究;2014年05期

9 劉長(zhǎng)庚;羅啟賢;凌天牖;莫業(yè)躍;程自力;黃生高;莫暉;;燈盞花對(duì)成骨細(xì)胞及破骨前體細(xì)胞OPG/RANKL/RANK表達(dá)的影響[J];中國(guó)中西醫(yī)結(jié)合雜志;2013年12期

10 崔斌;孫天勝;;腫瘤壞死因子和部分白介素對(duì)骨折影響的研究進(jìn)展[J];中華臨床醫(yī)師雜志(電子版);2013年21期

，

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