發(fā)布時(shí)間：2019-01-17 10:14

【摘要】：目的:觀察紫杉醇對(duì)裸鼠體內(nèi)涎腺腺樣囊性癌移植瘤的抑制作用。方法:按常規(guī)將涎樣囊性癌ACC-M細(xì)胞接種于裸鼠皮下,2周后將荷瘤鼠隨機(jī)分為2組(n=8),對(duì)治療組鼠皮下注射紫杉醇,10μg/g,1次/d。對(duì)照組鼠給予等量生理鹽水。定期測(cè)量腫瘤體積,治療2周后處死,測(cè)量腫瘤重量,病理學(xué)觀察,流式細(xì)胞術(shù)檢測(cè)各組凋亡率。免疫組化法檢測(cè)血管內(nèi)皮生長(zhǎng)因子(VEGF)在腫瘤細(xì)胞中的表達(dá)。結(jié)果:治療2周后紫杉醇治療組抑瘤率42.74%,治療組和對(duì)照組腫瘤細(xì)胞凋亡率分別為(24.3±2.1)%和(9.2±1.9)%;VEGF蛋白表達(dá)平均吸光度值(MOD)分別為0.322±0.033和0.388±0.029(P0.01)。結(jié)論:紫杉醇能夠抑制ACC-M細(xì)胞在裸鼠體內(nèi)的增殖,促進(jìn)其凋亡,抑制VEGF的表達(dá)。
[Abstract]:Aim: to observe the inhibitory effect of paclitaxel on adenoid cystic carcinoma of salivary gland in nude mice. Methods: ACC-M cells of salivary cystic carcinoma were subcutaneously inoculated into nude mice. After 2 weeks, tumor bearing mice were randomly divided into two groups (n = 8). Paclitaxel was injected subcutaneously into the treatment group with 10 渭 g / g paclitaxel once a day. Rats in the control group were given the same amount of normal saline. Tumor volume was measured regularly and killed after 2 weeks of treatment. Tumor weight, pathological observation and apoptosis rate were measured by flow cytometry. The expression of vascular endothelial growth factor (VEGF) in tumor cells was detected by immunohistochemistry. Results: after 2 weeks of treatment, the tumor inhibition rate of paclitaxel group was 42.74%, and the apoptosis rate of tumor cells in treatment group and control group was (24.3 鹵2.1)% and (9.2 鹵1.9)%, respectively. The average absorbance of VEGF protein expression was 0.322 鹵0.033 and 0.388 鹵0.029, respectively (P0.01). Conclusion: paclitaxel can inhibit the proliferation of ACC-M cells in nude mice, promote their apoptosis and inhibit the expression of VEGF.
【作者單位】： 昆明醫(yī)科大學(xué)附屬口腔醫(yī)院口腔頜面外科;
【基金】：云南省科技廳-昆明醫(yī)科大學(xué)聯(lián)合專項(xiàng)基金(編號(hào):2011FB211,2011FB163) 云南省高層次衛(wèi)生技術(shù)人才專項(xiàng)基金(編號(hào):D-201233)
【分類號(hào)】：R739.8

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