【摘要】：目的:探討純鈦種植體經(jīng)多巴胺修飾后加載環(huán)型RGD多肽復(fù)合涂層對成骨細(xì)胞生物行為的影響,比較不同純鈦表面處理后體外生物相容性的異同。方法:借助微弧氧化技術(shù)和化學(xué)偶聯(lián)技術(shù)在純鈦表面構(gòu)建陶瓷氧化膜-聚多巴胺-環(huán)RGD活性涂層,本實(shí)驗(yàn)分為三組,微弧氧化組為M組;微弧氧化加載多巴胺組為P組;微弧氧化加載多巴胺接枝環(huán)肽組為R組。采用掃描電鏡(SEM)觀察鈦片表面形貌;電子能譜(XPS)分析三組鈦片表面元素組成成分;常規(guī)條件下培養(yǎng)成骨細(xì)胞,CCK-8檢測成骨細(xì)胞在不同組別、不同時間點(diǎn)的黏附及增殖能力;熒光顯微鏡觀察成骨細(xì)胞24小時后在三組涂層表面的細(xì)胞形態(tài)。結(jié)果:掃描電鏡顯示表面形貌的改變,能譜分析顯示RGD環(huán)肽涂層加載成功,CCK8檢測結(jié)果顯示細(xì)胞的增殖及粘附,P0.05有統(tǒng)計(jì)學(xué)意義,且R組P組M組,熒光顯微鏡觀察R組、P組均較M組更利于細(xì)胞增殖和黏附,但R組更利于細(xì)胞鋪展,且細(xì)胞間聯(lián)系緊密。結(jié)論:三組實(shí)驗(yàn)中P組和R組都能有效促進(jìn)種植體的骨結(jié)合,其中R組效果更佳,對于種植體的涂層改進(jìn)有一定的臨床應(yīng)用意義。
[Abstract]:Aim: to investigate the effects of dopamine modified RGD polypeptide composite coating on the biological behavior of osteoblasts and compare the biocompatibility of titanium implants with different titanium surface treatments in vitro. Methods: ceramic oxide film polydopamine cyclic RGD active coating was constructed on the surface of pure titanium by means of microarc oxidation and chemical coupling technique. The experiment was divided into three groups: group M (group M), group P (group B) loaded by micro-arc oxidation (group P); The dopamine grafted cyclic peptide group was treated with microarc oxidation. Scanning electron microscope (SEM) was used to observe the surface morphology of titanium sheet, and (XPS) was used to analyze the composition of elements on the surface of three groups of titanium sheets. Osteoblasts were cultured under conventional conditions. CCK-8 was used to detect the adhesion and proliferation of osteoblasts in different groups and at different time points. The morphology of osteoblasts on the surface of the three groups was observed by fluorescence microscope after 24 hours. Results: scanning electron microscope showed the change of surface morphology, energy dispersive analysis showed that RGD cyclic peptide coating was loaded successfully, CCK8 test showed cell proliferation and adhesion, P 0.05, and group R P M, fluorescence microscope observation R group. Group P was more conducive to cell proliferation and adhesion than group M, but group R was more conducive to cell spreading and close relationship between cells. Conclusion: both group P and group R can promote the osseointegration of implants effectively in the three groups, and the effect of group R is better than that of group R, which has a certain clinical significance for the improvement of implant coating.
【學(xué)位授予單位】：佳木斯大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R783.1

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