【摘要】：目的:觀察咬合干擾后不同時(shí)間大鼠咬肌能量代謝產(chǎn)物腺嘌呤核苷三磷酸(adenosine triphosphate,ATP)、腺嘌呤核苷二磷酸(adenosine diphosphate,ADP)、次黃嘌呤核苷酸(inosine monophosphate,IMP)、磷酸肌酸、肌酸、乳酸及p H水平的變化,分析咬合干擾對(duì)咀嚼肌能量代謝的影響。方法:選用雄性Sprague-Dawley大鼠(220~250 g)50只,隨機(jī)分為實(shí)驗(yàn)組(40只)和對(duì)照組(10只),實(shí)驗(yàn)組于右上第一磨牙粘固0.4 mm厚金屬冠建立咬合干擾,并分別維持3、7、10、14 d(每個(gè)時(shí)間點(diǎn)各10只),對(duì)照組不施加咬合干擾。各組大鼠全麻下取雙側(cè)咬肌組織,其中5只大鼠樣本加入0.4 mol/L高氯酸(10 m L/g)充分勻漿,離心、過(guò)濾后采用高效液相色譜分析ATP、ADP、IMP、磷酸肌酸、肌酸及乳酸含量,另外5只大鼠樣本加入含5 mmol/L碘醋酸鈉的勻漿液(10 m L/g),充分勻漿后在37℃恒溫水浴環(huán)境中利用p H計(jì)測(cè)試p H值。結(jié)果:與對(duì)照組相比,大鼠雙側(cè)咬肌ATP含量在咬合干擾3 d[右側(cè):(5.36±0.13)μmol/g,左側(cè):(5.77±0.25)μmol/g]升高(P0.05),7、10和14 d沒(méi)有顯著改變;大鼠雙側(cè)咬肌IMP[右側(cè):(0.21±0.03)μmol/g,左側(cè):(0.19±0.03)μmol/g]、肌酸[右側(cè):(24.76±2.94)μmol/g,左側(cè):(27.75±2.23)μmol/g]含量在咬合干擾7 d升高(P0.05),3、10和14 d沒(méi)有顯著改變;大鼠雙側(cè)咬肌磷酸肌酸含量在咬合干擾7、10和14 d降低[右側(cè)分別為:(10.70±0.71)μmol/g、(11.57±0.52)μmol/g、(10.74±1.39)μmol/g,左側(cè)分別為:(10.05±0.57)μmol/g、(10.75±1.12)μmol/g、(10.61±1.15)μmol/g,P0.05],3 d沒(méi)有顯著改變;大鼠雙側(cè)咬肌ADP、乳酸含量及p H水平在咬合干擾后各時(shí)間點(diǎn)均沒(méi)有顯著改變(P0.05)。結(jié)論:咬合干擾導(dǎo)致大鼠咀嚼肌能量代謝產(chǎn)物含量改變,可能與咬合干擾誘發(fā)咀嚼肌疼痛、功能紊亂、肌纖維構(gòu)筑改變等病理過(guò)程相關(guān)。
[Abstract]:Objective: to observe the energy metabolites of masseter muscle of rats at different time after occlusal interference: adenosine triphosphate (adenosine triphosphate,ATP), adenosine diphosphate (adenosine diphosphate,ADP), Hypoxanthine nucleotide (inosine monophosphate,IMP), creatine phosphate (creatine phosphate). The changes of lactic acid and pH levels and the effects of occlusal interference on energy metabolism of masticatory muscles were analyzed. Methods: 50 male Sprague-Dawley rats (220 250g) were randomly divided into two groups: experimental group (40 rats) and control group (10 rats). The occlusion interference was established in the right first molar with 0.4 mm thick metal crown. Ten minutes and 14 days (10 rats in each time point), the control group did not exert occlusion interference. Bilateral masseter muscle tissues were taken from rats in each group under general anesthesia. The samples of 5 rats were added with 0.4 mol/L perchloric acid (10 mL / g) to fully homogenate, centrifuged, filtered, and then analyzed by high performance liquid chromatography (HPLC) for creatine phosphate. The contents of creatine and lactic acid in the other 5 rats were added to the homogenate solution containing 5 mmol/L sodium iodide acetate (10 mL / g), and then pH was measured by pH meter in 37 鈩，

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