【摘要】：研究目的:通過建立SD大鼠上頜第一磨牙無髓根管模型并刺激損傷根尖周組織出血,觀察在刺激損傷根尖周后根管內(nèi)的組織變化特點(diǎn)、基質(zhì)細(xì)胞衍生因子1(stromal cell derived factor-1,SDF-1)在根管內(nèi)表達(dá)情況及損傷前后血清中SDF-1濃度變化。探討牙髓血管再生中SDF-1的作用。研究方法:6-8周齡SPF級(jí)別SD雄性大鼠64只,體重180-220g。建立左側(cè)上頜第一磨牙無髓根管模型后對(duì)實(shí)驗(yàn)動(dòng)物進(jìn)行根尖周刺激損傷,誘導(dǎo)根尖出血(56只)。對(duì)損傷組刺激近中根根尖損傷出血并按照根尖周損傷后不同的時(shí)間點(diǎn)隨機(jī)分為損傷12h、1d、2d、4d、7d、10d、14d共7組,麻醉后處死動(dòng)物并采集血液及上頜骨,酶聯(lián)免疫吸附試驗(yàn)(enzyme linked immunosorbent assay,ELISA)檢測(cè)血清中SDF-1含量。上頜骨固定、脫鈣后行組織化學(xué)染色法觀察在根尖周損傷后根管內(nèi)組織的變化情況及SDF-1在根管內(nèi)組織的表達(dá)特點(diǎn)。研究結(jié)果:進(jìn)入根管的根尖血液發(fā)生凝血,在損傷后1d、2d及4d,根管內(nèi)組織表現(xiàn)形式為纖維蛋白包裹血凝塊及血細(xì)胞。組織損傷后期,根管內(nèi)血凝塊溶解及炎細(xì)胞減少,組織之間及組織與牙本質(zhì)之間連接緊密,損傷后14天組織中出現(xiàn)新生血管結(jié)構(gòu),組織與根管壁之間出現(xiàn)細(xì)胞結(jié)構(gòu)。免疫組織化學(xué)染色顯示,創(chuàng)傷后根管內(nèi)組織的血凝塊及炎性細(xì)胞表達(dá)SDF-1,隨著時(shí)間增加,血凝塊及炎性細(xì)胞減少,SDF-1表達(dá)減弱。ELISA檢測(cè)顯示,根尖周損傷后,12h組及4 d組血清中SDF-1濃度升高(P0.05),差異有統(tǒng)計(jì)學(xué)意義,其余時(shí)間段結(jié)果與損傷前相比無顯著性變化。研究結(jié)論:1.根尖誘導(dǎo)出血后,根管內(nèi)牙髓血管再生的過程及損傷后SDF-1在根管內(nèi)組織的表達(dá)與血凝塊和炎細(xì)胞狀態(tài)相關(guān)。2.根尖周損傷后12h及4d,血清中SDF-1濃度升高,但在整個(gè)牙髓血管再生過程未能持續(xù),通過刺激根尖周損傷趨化骨髓內(nèi)干細(xì)胞通過血循環(huán)歸巢的證據(jù)需進(jìn)一步實(shí)驗(yàn)才能證實(shí)。
[Abstract]:Objective: to observe the changes of tissue and stromal cell derived factor 1 (stromal cell derived factor-1,) in the root canal of maxillary first molar induced by SD by stimulating periapical hemorrhage. SDF-1) expression in root canal and the change of serum SDF-1 concentration before and after injury. To investigate the role of SDF-1 in dental pulp vascular regeneration. Methods: 64 SPF grade SD male rats of 6-8 weeks old, weight 180-220 g. The left maxillary first molar model of unmyelinated root canal was established and the periapical injury was carried out in the experimental animals (56 rats). The injury group was randomly divided into 7 groups according to different time points after periapical injury. The animals were killed and blood and maxillary bone were collected after anesthesia. Enzyme linked immunosorbent assay (enzyme linked immunosorbent assay,ELISA) was used to detect the content of SDF-1 in serum. The changes of root canal tissue and the expression of SDF-1 in root canal were observed by histochemical staining after maxillary fixation and decalcification. The results showed that the blood clotting in the root tip of the root canal showed fibrin in the blood clots and blood cells in the root canal at 2 and 4 days after the injury. In the late stage of tissue injury, hemagglutination and inflammatory cells in the root canal decreased, and the connection between tissue and dentin was close. On the 14th day after injury, neovascularization appeared in the tissue and cell structure appeared between the tissue and the root canal wall. Immunohistochemical staining showed that the expression of SDF-1, in blood clots and inflammatory cells in root canal after trauma increased with time, blood clots and inflammatory cells decreased, and SDF-1 expression decreased. ELISA detection showed that after periapical injury, the expression of SDF-1, was decreased. The serum SDF-1 concentration in 12h group and 4-day group was significantly higher than that before injury (P0.05). Conclusion: 1. The process of pulp vascular regeneration in root canal and the expression of SDF-1 in root canal were related to blood clots and inflammatory cells after apical hemorrhage. 2. The concentration of SDF-1 in serum increased at 12 h and 4 d after periapical injury, but it could not be sustained in the whole process of pulp vascular regeneration. The evidence of blood circulation homing by stimulating periapical injury to chemotaxis of bone marrow stem cells could not be confirmed by further experiments.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R781

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