【摘要】：目的:對(duì)比基質(zhì)細(xì)胞衍生因子1(stromal cell-derived factor-1,SDF-1)和粒細(xì)胞集落刺激因子(granulocyte colony-stimulating factor,G-CSF)對(duì)人牙髓干細(xì)胞(dental pulp stem cell,DPSC)的體外增殖、遷移和成牙本質(zhì)能力的影響。方法:分別在培養(yǎng)基中加入100μg/L SDF-1或100μg/L G-CSF,采用細(xì)胞計(jì)數(shù)試劑盒(cell counting kit-8,CCK-8)和集落形成實(shí)驗(yàn)(colony-forming unit,CFU)檢測(cè)SDF-1和G-CSF對(duì)DPSC增殖的影響;采用劃痕實(shí)驗(yàn)和Transwell遷移實(shí)驗(yàn)檢測(cè)兩者對(duì)DPSC遷移能力的影響;對(duì)DPSC進(jìn)行成牙本質(zhì)誘導(dǎo),通過堿性磷酸酶(alkaline phosphatase,ALP)染色、測(cè)定ALP活性、茜素紅染色和real-time RT-PCR檢測(cè)成牙本質(zhì)相關(guān)基因的表達(dá),以檢測(cè)兩者對(duì)DPSC體外成牙本質(zhì)能力的影響。結(jié)果:SDF-1和G-CSF能夠輕度提高DPSC的增殖及集落形成能力,但差異無統(tǒng)計(jì)學(xué)意義。加入SDF-1或G-CSF的實(shí)驗(yàn)組劃痕匯合速率明顯高于對(duì)照組(P0.01),但兩種因子間差異無統(tǒng)計(jì)學(xué)意義。Transwell遷移實(shí)驗(yàn)中,對(duì)照組每視野的遷移細(xì)胞數(shù)量為(5.0±1.4)個(gè),SDF-1組每視野的遷移細(xì)胞數(shù)量為(24.3±6.8)個(gè),G-CSF組每視野的遷移細(xì)胞數(shù)量為(11.8±3.3)個(gè),各組間差異有統(tǒng)計(jì)學(xué)意義(P0.05)。經(jīng)成牙本質(zhì)誘導(dǎo)后,實(shí)驗(yàn)組細(xì)胞ALP染色加深,ALP活性上升,礦化結(jié)節(jié)形成數(shù)量增加,成牙本質(zhì)相關(guān)基因的表達(dá)均顯著高于對(duì)照組。結(jié)論:SDF-1對(duì)DPSC的增殖能力影響不顯著,但能明顯提高DPSC的遷移能力和成牙本質(zhì)分化能力,效果優(yōu)于G-CSF。
[Abstract]:Aim: to compare the proliferation of human dental pulp stem cells (dental pulp stem cell,DPSC) by stromal cell derived factor 1 (stromal cell-derived factor-1,SDF-1) and granulocyte colony stimulating factor (granulocyte colony-stimulating factor,G-CSF) in vitro. Effects of migration and dentin formation. Methods: 100 渭 g / L SDF-1 or 100 渭 g / L G-CSF were added to the medium, respectively. The cell count kit (cell counting kit-8,CCK-8) and colony forming assay (colony-forming unit,) were used. CFU) was used to detect the effect of SDF-1 and G-CSF on the proliferation of DPSC. The effects of scratch test and Transwell migration test on the migration ability of DPSC were investigated. DPSC was induced by dentin formation. The activity of ALP was determined by alkaline phosphatase (alkaline phosphatase,ALP) staining. The expression of dentin related genes was detected by alizarin red staining and real-time RT-PCR. To detect the effect of both on dentin formation ability of DPSC in vitro. Results: SDF-1 and G-CSF could slightly improve the proliferation and colony formation of DPSC, but the difference was not statistically significant. The rate of scratch convergence in the experimental group added with SDF-1 or G-CSF was significantly higher than that in the control group (P0.01), but there was no significant difference between the two factors. In the Transwell migration experiment, the number of migration cells per field in the control group was (5.0 鹵1.4). The number of migration cells per field was (24.3 鹵6.8) in SDF-1 group and (11.8 鹵3.3) in G-CSF group (P0.05). After dentin induction, ALP staining deepened, ALP activity increased, the number of mineralized nodules increased, and the expression of dentin related genes in the experimental group was significantly higher than that in the control group. Conclusion: SDF-1 has no significant effect on the proliferation of DPSC, but it can obviously improve the migration ability and dentin differentiation ability of DPSC, and the effect is better than that of G-CSF.
【作者單位】： 北京大學(xué)口腔醫(yī)學(xué)院·口腔醫(yī)院兒童口腔科;
【基金】：國家自然科學(xué)基金(81170928) 北京大學(xué)臨床醫(yī)院合作專項(xiàng)(2013-4-01)資助~~
【分類號(hào)】：R781

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王嘉德,R.Hume;酸和堿性物質(zhì)與牙本質(zhì)滲透性之間相互作用的研究[J];北京醫(yī)科大學(xué)學(xué)報(bào);1990年01期

2 孟令強(qiáng);平雅坤;;遺傳性牙本質(zhì)生成不全一家族報(bào)告[J];現(xiàn)代口腔醫(yī)學(xué)雜志;1992年04期

3 段建民;對(duì)牙本質(zhì)橋作為活髓保存成功標(biāo)志的辯證認(rèn)識(shí)[J];牙體牙髓牙周病學(xué)雜志;1996年02期

4 王雙運(yùn),聶解初,賓麗華,胡安元,趙海興;部分脫抗原牙本質(zhì)移植后光鏡電鏡觀察[J];臨床口腔醫(yī)學(xué)雜志;2000年04期

5 趙高峰;黃向東;汪昌寧;宋亞玲;邊專;;牙本質(zhì)發(fā)育異常家系調(diào)查和表型分析[J];口腔醫(yī)學(xué)研究;2006年04期

6 王浩洋;崔英霞;黃宇烽;;牙本質(zhì)發(fā)育異常和牙本質(zhì)發(fā)育不全分子遺傳學(xué)的研究進(jìn)展[J];中國優(yōu)生與遺傳雜志;2009年01期

7 胡鑫瑜;王浩洋;夏毅;;牙本質(zhì)唾液酸焦磷酸蛋白和牙本質(zhì)[J];中國優(yōu)生與遺傳雜志;2010年12期

8 蔣維中;介紹一種新的牙本質(zhì)掃描電鏡樣品的制備和觀察方法[J];牙體牙髓牙周病學(xué)雜志;1995年02期

9 李海清;牛玉梅;線恒澤;;氯化鈉溶液對(duì)牙本質(zhì)再礦化作用的影響[J];口腔醫(yī)學(xué)研究;2006年02期

10 楊東梅;李玉晶;宿穎;孫異臨;;宿主來源的基質(zhì)金屬蛋白酶對(duì)根部牙本質(zhì)膠原的降解作用[J];中華口腔醫(yī)學(xué)雜志;2006年05期

相關(guān)會(huì)議論文 前10條

1 梁國斌;李彥;;均衡樣本剩余牙本質(zhì)厚度組間差異的實(shí)驗(yàn)探討[A];第六次全國口腔修復(fù)學(xué)學(xué)術(shù)會(huì)議論文摘要匯編[C];2009年

2 張彥博;李彥;;水儲(chǔ)存對(duì)牙本質(zhì)樹脂粘結(jié)耐久性的影響[A];第六次全國口腔修復(fù)學(xué)學(xué)術(shù)會(huì)議論文摘要匯編[C];2009年

3 崔英霞;侯燕寧;王云華;郝麗君;拜紅霞;李芳秋;黃宇烽;;1個(gè)牙本質(zhì)生長不全Ⅱ型家系疾病基因的連鎖分析[A];第十屆全軍檢驗(yàn)醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2005年

4 李秋紅;王如;;兩種粘結(jié)系統(tǒng)對(duì)牙本質(zhì)處理獲得混合層不脫鈣磨片觀察[A];中華口腔醫(yī)學(xué)會(huì)全科口腔醫(yī)學(xué)專業(yè)委員會(huì)第一次學(xué)術(shù)年會(huì)會(huì)議論文集[C];2009年

5 胡曉莉;彭延文;Sum Cheepeng;凌均h，

本文編號(hào)：2391801