【摘要】：舌鱗狀細胞癌是高度轉移性并具有鱗狀細胞分化潛能的上皮性腫瘤。癌細胞通常會向周圍神經血管等正常組織浸潤,也會發(fā)生頸部淋巴結的轉移,淋巴結轉移多數(shù)情況下在病變發(fā)生的同側區(qū)域,但是當病變組織位于中線處或者病變已經累及至中線時,腫瘤細胞則有向對側區(qū)域或者雙側轉移的趨勢。臨床上根據(jù)腫瘤大小,部位及轉移擴散范圍確定TNM分期以明確治療方案,目前多主張采用手術聯(lián)合放化療的綜合序列治療的方式,目前學者們正積極地向個性化基因靶向治療的方向進行研究。 本研究以慢病毒為載體通過RNAi技術轉染COX-2基因穩(wěn)定沉默后對Tca8113細胞株的成瘤性及其侵襲轉移能力的影響為目的,初探COX-2基因沉默對人舌鱗狀細胞癌作用的可能機制。選取穩(wěn)定傳代的活細胞分別設置COX-2基因沉默組(pLKO.1shCOX-2)、空載體組(Tca8113scr)和未處理組(Tca8113)并對其進行常規(guī)細胞培養(yǎng),采用Western-blot驗證COX-2蛋白的沉默效率；采用細胞克隆形成實驗檢測成瘤能力的變化；通過Transwell小室的侵襲實驗和遷移實驗檢測COX-2基因沉默后侵襲轉移能力的變化。 通過以上實驗研究主要得到了以下結論： (1)慢病毒轉染的Tca8113細胞株COX-2蛋白表達明顯降低； (2)COX-2基因沉默后可以有效地抑制Tca8113細胞株的成瘤情況； (3)COX-2基因沉默后降低了Tca8113細胞株的侵襲轉移能力。
[Abstract]:Tongue squamous cell carcinoma is a highly metastatic epithelial tumor with squamous cell differentiation potential. Cancer cells usually infiltrate into normal tissues such as peripheral nerve and blood vessels, and neck lymph nodes metastasize, most often in the ipsilateral region of the lesion. However, when the lesion tissue is located at the midline or the lesion has been involved in the midline, the tumor cells tend to metastasize to the contralateral region or to both sides. According to the tumor size, location and the range of metastasis and diffusion, TNM staging was determined to determine the treatment plan. At present, it is advocated to adopt the comprehensive sequence therapy of surgery combined with radiotherapy and chemotherapy. At present, scholars are actively conducting research in the direction of individualized gene targeted therapy. The aim of this study was to explore the possible mechanism of COX-2 gene silencing on human tongue squamous cell carcinoma (TSCC) in order to investigate the effect of lentivirus transfection of COX-2 gene on tumorigenesis and invasion and metastasis of Tca8113 cell line by RNAi technique. COX-2 gene silencing group (pLKO.1shCOX-2), empty vector group (Tca8113scr) and untreated group (Tca8113) were selected and cultured by routine cell culture. Western-blot was used to verify the silencing efficiency of COX-2 protein. Cell clone formation assay was used to detect the change of tumorigenic ability, and the invasion and metastasis ability of COX-2 gene after silencing was detected by Transwell chamber invasion test and migration assay. The main conclusions were as follows: (1) the expression of COX-2 protein in lentivirus transfected Tca8113 cell line was significantly decreased; (2) COX-2 gene silencing could effectively inhibit the tumorigenesis of Tca8113 cell line. (3) COX-2 gene silencing decreased the invasion and metastasis ability of Tca8113 cell line.
【學位授予單位】：蘭州大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R739.86

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