【摘要】：舌鱗狀細(xì)胞癌是高度轉(zhuǎn)移性并具有鱗狀細(xì)胞分化潛能的上皮性腫瘤。癌細(xì)胞通常會(huì)向周圍神經(jīng)血管等正常組織浸潤(rùn),也會(huì)發(fā)生頸部淋巴結(jié)的轉(zhuǎn)移,淋巴結(jié)轉(zhuǎn)移多數(shù)情況下在病變發(fā)生的同側(cè)區(qū)域,但是當(dāng)病變組織位于中線處或者病變已經(jīng)累及至中線時(shí),腫瘤細(xì)胞則有向?qū)?cè)區(qū)域或者雙側(cè)轉(zhuǎn)移的趨勢(shì)。臨床上根據(jù)腫瘤大小,部位及轉(zhuǎn)移擴(kuò)散范圍確定TNM分期以明確治療方案,目前多主張采用手術(shù)聯(lián)合放化療的綜合序列治療的方式,目前學(xué)者們正積極地向個(gè)性化基因靶向治療的方向進(jìn)行研究。 本研究以慢病毒為載體通過RNAi技術(shù)轉(zhuǎn)染COX-2基因穩(wěn)定沉默后對(duì)Tca8113細(xì)胞株的成瘤性及其侵襲轉(zhuǎn)移能力的影響為目的,初探COX-2基因沉默對(duì)人舌鱗狀細(xì)胞癌作用的可能機(jī)制。選取穩(wěn)定傳代的活細(xì)胞分別設(shè)置COX-2基因沉默組(pLKO.1shCOX-2)、空載體組(Tca8113scr)和未處理組(Tca8113)并對(duì)其進(jìn)行常規(guī)細(xì)胞培養(yǎng),采用Western-blot驗(yàn)證COX-2蛋白的沉默效率；采用細(xì)胞克隆形成實(shí)驗(yàn)檢測(cè)成瘤能力的變化；通過Transwell小室的侵襲實(shí)驗(yàn)和遷移實(shí)驗(yàn)檢測(cè)COX-2基因沉默后侵襲轉(zhuǎn)移能力的變化。 通過以上實(shí)驗(yàn)研究主要得到了以下結(jié)論： (1)慢病毒轉(zhuǎn)染的Tca8113細(xì)胞株COX-2蛋白表達(dá)明顯降低； (2)COX-2基因沉默后可以有效地抑制Tca8113細(xì)胞株的成瘤情況； (3)COX-2基因沉默后降低了Tca8113細(xì)胞株的侵襲轉(zhuǎn)移能力。
[Abstract]:Tongue squamous cell carcinoma is a highly metastatic epithelial tumor with squamous cell differentiation potential. Cancer cells usually infiltrate into normal tissues such as peripheral nerve and blood vessels, and neck lymph nodes metastasize, most often in the ipsilateral region of the lesion. However, when the lesion tissue is located at the midline or the lesion has been involved in the midline, the tumor cells tend to metastasize to the contralateral region or to both sides. According to the tumor size, location and the range of metastasis and diffusion, TNM staging was determined to determine the treatment plan. At present, it is advocated to adopt the comprehensive sequence therapy of surgery combined with radiotherapy and chemotherapy. At present, scholars are actively conducting research in the direction of individualized gene targeted therapy. The aim of this study was to explore the possible mechanism of COX-2 gene silencing on human tongue squamous cell carcinoma (TSCC) in order to investigate the effect of lentivirus transfection of COX-2 gene on tumorigenesis and invasion and metastasis of Tca8113 cell line by RNAi technique. COX-2 gene silencing group (pLKO.1shCOX-2), empty vector group (Tca8113scr) and untreated group (Tca8113) were selected and cultured by routine cell culture. Western-blot was used to verify the silencing efficiency of COX-2 protein. Cell clone formation assay was used to detect the change of tumorigenic ability, and the invasion and metastasis ability of COX-2 gene after silencing was detected by Transwell chamber invasion test and migration assay. The main conclusions were as follows: (1) the expression of COX-2 protein in lentivirus transfected Tca8113 cell line was significantly decreased; (2) COX-2 gene silencing could effectively inhibit the tumorigenesis of Tca8113 cell line. (3) COX-2 gene silencing decreased the invasion and metastasis ability of Tca8113 cell line.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R739.86

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