【摘要】：目的：調(diào)查致齲相關(guān)變形鏈球菌(Mutans Streptococci, MS)在喀什封閉維吾爾族不同齲敏感兒童口腔菌斑中基因型分布狀況,初探其與低齡嬰幼兒齲(EarlyChildhood Caries, ECC)相關(guān)性,為兒童齲病病因?qū)W研究提供依據(jù)。方法：以24～71個月齡維吾爾族封閉人群兒童為研究對象,選符合取樣標(biāo)準(zhǔn)的32例重度嬰幼兒齲患兒(S-ECC組),32例無齲兒童(CF組),收集口腔菌斑樣本行選擇性細(xì)菌培養(yǎng),根據(jù)形態(tài)學(xué)特征分離并分子生物學(xué)方法進(jìn)一步鑒定變異鏈球菌(Streptococcus mutans,Sm)及遠(yuǎn)緣鏈球菌(Streptococcus sobrinus, Ss),利用隨機(jī)引物聚合酶鏈反應(yīng)(Arbitraryprimed-PCR, AP-PCR)對兩種人類致齲相關(guān)變異鏈球菌屬臨床分離株基因組DNA行基因型分析。結(jié)果：兩組兒童Sm檢出率為82.8%(53/64), Ss檢出率為29.7%(19/64), Sm及Ss檢出率在兩組間差異無統(tǒng)計學(xué)意義(P0.05)。SECC及CF組個體Sm基因型分別為1～6種及1～5種,Ss基因型為1～3種。S-ECC組中攜帶一種以上Sm基因型的個體占75%,攜帶一種以上Ss基因型的個體占15.7%；CF組攜帶一種以上Sm基因型的個體占59.4%,攜帶一種以上Ss基因型的個體占12.5%。S-ECC變形鏈球菌基因多態(tài)性顯著高于CF組(P0.05)。Sm基因型數(shù)與dmft呈正相關(guān)(r=0.439, P0.05)。Logistic回歸分析Sm及Ss基因多態(tài)性與不同齲敏感性關(guān)系,發(fā)現(xiàn)Sm基因多態(tài)性對疾病的發(fā)生影響是有統(tǒng)計學(xué)意義的(B=0.4040, P0.05=。結(jié)論：Sm, Ss在封閉維吾爾族不同齲敏感兒童菌斑檢出率無明顯差異,Sm, Ss臨床菌株間存在基因多態(tài)性且SECC組基因型數(shù)顯著高于CF組,個體攜帶Sm基因型種類數(shù)與其致齲性之間存在相關(guān)性而與攜帶Ss基因型數(shù)無明顯相關(guān)性。個體攜帶Sm基因型越多,可能會增加患齲危險。
[Abstract]:Objective: to investigate the genotypic distribution of cariogenic streptococcus mutans (Mutans Streptococci, MS) in oral plaque of Uygur children with different caries sensitivity in Kashi, and to explore its correlation with (EarlyChildhood Caries, ECC) in young children with caries. To provide the basis for the study of the etiology of caries in children. Methods: a total of 32 children with severe caries (S-ECC group) and 32 children without caries (CF group) were enrolled in this study. Oral plaque samples were collected for selective bacterial culture and further identification of Streptococcus mutans (Streptococcus mutans,Sm) and Streptococcus distalis (Streptococcus sobrinus, Ss),) by molecular biological methods. Genomic DNA of two clinical isolates of Streptococcus mutans were genotyped by random primer polymerase chain reaction (Arbitraryprimed-PCR, AP-PCR). Results: the detection rate of Sm in the two groups was 82.8% (53 / 64), Ss was 29.7%). There was no significant difference in the detection rate of Sm and Ss between the two groups (P0.05) in the). SECC and CF groups, there were 6 Sm genotypes and 5 Sm genotypes, respectively. In S-ECC group, 75 individuals carried more than one Sm genotype, and 15.7individuals carrying more than one Ss genotype. The percentage of individuals with more than one Sm genotype in CF group was 59.4%. The genetic polymorphisms of Streptococcus mutans in 12.5%.S-ECC with more than one Ss genotype were significantly higher than those in CF group (P0.05). There was a positive correlation between the number of). Sm genotypes and dmft (r = 0.439, P < 0.05). P0.05). Logistic regression analysis showed that the polymorphism of Sm and Ss gene was associated with different caries susceptibility, and the influence of Sm gene polymorphism on the occurrence of the disease was statistically significant (BX 0.4040, P0.05 0. 05). Conclusion there was no significant difference in the detection rate of plaque in Uygur children with different caries sensitivity by: Sm, Ss. There was no significant difference in gene polymorphism among clinical strains of, Sm, Ss and the number of genotypes in SECC group was significantly higher than that in CF group. There was a correlation between the number of genotypes carrying Sm and caries, but not with the number of genotypes carrying Ss. The more individuals carry Sm genotypes, the higher the risk of caries may be.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R788.1

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本文編號：2352307