【摘要】：[目的]研究氟保護(hù)漆Duraphat對(duì)碳酸飲料引起恒牙釉質(zhì)脫礦后再礦化的影響及作用,為碳酸飲料導(dǎo)致恒牙釉質(zhì)脫礦的治療提供實(shí)驗(yàn)依據(jù)和臨床參考。[方法]1.碳酸飲料對(duì)恒牙釉質(zhì)脫礦作用的體外研究：選擇12歲-25歲因正畸減數(shù)拔除的前磨牙15顆,每顆離體牙制備2個(gè)釉質(zhì)切片,2個(gè)釉質(zhì)切片隨機(jī)分入A、B組,并編號(hào),每組15個(gè)樣本,分別用可口可樂(lè)(A組)和去離子水(B組)處理釉質(zhì)切片10min,取出再置于人工唾液處理1h,如此每天循環(huán)4次,共7天,整個(gè)實(shí)驗(yàn)過(guò)程在37℃恒溫箱中完成后,采用掃描電鏡觀察釉質(zhì)表面形態(tài)變化,X線能譜分析恒牙釉質(zhì)表面的鈣、磷水平并分析比較其變化。2.氟保護(hù)漆Duraphat對(duì)恒牙釉質(zhì)脫礦后再礦化影響的體外研究：選擇12歲-25歲因正畸減數(shù)拔除的前磨牙15顆,每顆離體牙制備3個(gè)釉質(zhì)切片,3個(gè)釉質(zhì)切片隨機(jī)分入A、B、C組,并編號(hào),A組為Duraphat組,B組為2%NaF組,C組為空白對(duì)照組,每組15個(gè)樣本。3組樣本均進(jìn)行可口可樂(lè)處理10min,取出再置于人工唾液處理1h,如此每天循環(huán)4次,共7天,A組樣本涂布Duraphat, B組樣本涂布2%NaF,C組未處理,將3組樣本置于人工唾液中7天,整個(gè)過(guò)程在37℃恒溫箱中完成,采用掃描電鏡觀察恒釉質(zhì)表面形態(tài)變化,X線能譜分析釉質(zhì)表面的鈣、磷水平并分析比較其變化。[結(jié)果]1.碳酸飲料對(duì)恒牙釉質(zhì)脫礦作用的體外研究：掃描電鏡顯示可口可樂(lè)組恒牙釉質(zhì)表面粗糙,呈蜂窩狀外觀；去離子水組恒牙釉質(zhì)表面平坦。X線能譜分析恒牙釉質(zhì)表面鈣、磷水平結(jié)果顯示可口可樂(lè)組鈣：41.47±3.73,磷：18.17±0.77,去離子水組鈣：45.16±3.67,磷：18.67±0.44,鈣、磷水平兩組間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。2.氟保護(hù)漆Duraphat對(duì)恒牙釉質(zhì)脫礦后再礦化影響的體外研究：掃描電鏡顯示Duraphat組釉質(zhì)表面見大面積沉積物,2%NaF組釉質(zhì)表面見細(xì)小的沉積物,人工唾液組釉質(zhì)表面見少許沉積物。X線能譜分析3組恒牙釉質(zhì)表面鈣、磷水平結(jié)果顯示Duraphat組鈣：39.72±3.94,磷：18.24±0.47；2%NaF組鈣：39.54±2.40,磷：17.70±0.71；空白對(duì)照組鈣：36.85±2.87,磷：17.70±0.71。鈣、磷水平Duraphat組和空白對(duì)照組間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05),鈣水平2%NaF組和空白對(duì)照組間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05),磷水平2%NaF組和空白對(duì)照組間比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),鈣、磷水平Duraphat組和2%NaF組兩組之間比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。[結(jié)論]1.本實(shí)驗(yàn)條件下碳酸飲料對(duì)恒牙釉質(zhì)具有明顯的脫礦作用。2.本實(shí)驗(yàn)條件下氟保護(hù)漆Duraphat可促進(jìn)碳酸飲料引起恒牙釉質(zhì)脫礦的再礦化。
[Abstract]:[objective] to study the effect of fluorine protective paint (Duraphat) on demineralization of permanent enamel induced by carbonated beverage, and to provide experimental basis and clinical reference for the treatment of permanent enamel demineralization caused by carbonated beverage. [methods] 1. In vitro study on demineralization of permanent enamel by carbonated beverage: 15 premolars aged from 12 to 25 years old were selected for extraction due to orthodontic meiosis. Two enamel sections were prepared from each tooth, and 2 enamel slices were randomly divided into AHB group and numbered. 15 samples of each group were treated with Coca Cola (group A) and deionized water (group B) for 10 minutes and then treated with artificial saliva for 1 hour. After the whole experiment was completed in 37 鈩，

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