阿霉素聯(lián)合二甲雙胍應用對舌癌CAL27細胞增殖及凋亡的影響
發(fā)布時間：2018-11-16 10:47
【摘要】：目的:研究阿霉素(ADM)與二甲雙胍(MET)單獨應用及二者聯(lián)合用藥對體外培養(yǎng)的舌癌CAL27細胞增殖以及凋亡的影響。方法:1.依次采用MET藥物濃度5mmol/L、10mmol/L、20mmol/L、40mmol/L、80mmol/L、160mmol/L。ADM藥物濃度0.02mg/L、0.04mg/L、0.08mg/L、0.16mg/L、0.32mg/L。分別對體外培養(yǎng)的舌癌CAL27細胞作用24h、48h、72h后,用CCK-8法檢測各用藥組的吸光度值,算出細胞的抑制率,利用SPSS 17.0軟件計算出兩組藥物單獨使用時抑制50%細胞生長的濃度IC50;而聯(lián)合用藥組則采取單獨用藥時所得的ADM組IC50+MET組IC50處理CAL27細胞48h后上酶標儀檢測吸光度值,計算出聯(lián)合用藥組的細胞抑制率;2.根據(jù)CCK-8實驗組數(shù)據(jù),選取48h MET半抑制濃度10mmol/L,ADM半抑制濃度0.05mg/L及二者聯(lián)合作用于CAL27細胞后,用流式細胞術檢測各實驗組細胞凋亡的情況;3.采用q RT-PCR技術檢測MET 10mmol/L,ADM 0.05mg/L及MET 10mmol/L+ADM 0.05mg/L聯(lián)合應用48h后舌癌CAL27細胞的相關凋亡基因Bax及Bcl-2 m RNA相對表達的情況。結(jié)果:1.CCK-8數(shù)據(jù)顯示MET與ADM單獨作用24h、48h、72h后,均對舌癌CAL27細胞的增殖產(chǎn)生抑制作用,并且在一定濃度范圍內(nèi)呈劑量與時間的依賴性,二者聯(lián)合應用時比單獨用藥對CAL27細胞的增殖抑制作用更加明顯。2.流式細胞術結(jié)果顯示經(jīng)MET 10mmol/L+ADM 0.05mg/L聯(lián)合用藥后比單獨藥物組更能促進細胞的凋亡。3.q RT-PCR顯示MET及ADM二者合用時舌癌CAL27細胞的抗細胞凋亡基因Bcl-2的相對表達水平與單獨用藥組相比下調(diào)趨勢更明顯,而促細胞凋亡基因Bax m RNA的相對表達與單獨用藥組相比上調(diào)趨勢更明顯。結(jié)論:二甲雙胍作為降血糖藥物與阿霉素作用相似,單獨使用時具有對舌癌CAL27細胞體外增殖的抑制作用。當二者聯(lián)合應用時,對舌癌CAL27細胞的增殖抑制及促進凋亡的效果比單獨用藥更為顯著。
[Abstract]:Aim: to study the effects of adriamycin (ADM) and metformin (MET) on the proliferation and apoptosis of tongue cancer CAL27 cells cultured in vitro. Methods: 1. The drug concentration of MET was 5 mmol / L 10 mmol / L and 20 mmol / L = 40 mmol / L = 80 mmol / L = 160 mmol / L = 0.02 mg / L 0.04 mg / L 0.08 mg / L = 0.16 mg / L = 0.32 mg / L. After cultured tongue cancer CAL27 cells were treated for 24 h or 48 h for 72 h, the absorbance of each drug group was measured by CCK-8 method, and the inhibition rate of the cells was calculated. SPSS 17.0 software was used to calculate the concentration of IC50; that inhibited 50% cell growth in two groups of drugs alone. However, in the combined treatment group, the CAL27 cells in the IC50 MET group were treated with IC50 for 48 hours, and the absorbance value was measured by enzyme labeling instrument, and the cell inhibition rate of the combined treatment group was calculated. 2. According to the data of CCK-8 experimental group, 10 mmol / L MET semi-inhibitory concentration (0.05mg/L) of 48 h and their combination were used to detect the apoptosis of CAL27 cells by flow cytometry. 3. Q RT-PCR technique was used to detect the relative expression of Bax and Bcl-2 m RNA in CAL27 cells of tongue cancer treated with MET 10 mmol / L 0.05mg/L and MET 10mmol/L ADM 0.05mg/L for 48 h. Results: 1.CCK-8 data showed that the proliferation of tongue cancer CAL27 cells was inhibited by MET and ADM alone for 24 h or 48 h or 72 h, and in a dose-dependent and time-dependent manner. The inhibitory effect of the two drugs on the proliferation of CAL27 cells was more obvious than that of the drug alone. 2. 2. The results of flow cytometry showed that MET 10mmol/L ADM 0.05mg/L combined with drugs could promote cell apoptosis more than that of single drug group. 3. Q RT-PCR showed antiapoptotic genes in CAL27 cells of tongue cancer combined with MET and ADM. The relative expression level of Bcl-2 was more down-regulated than that of the control group. The relative expression of apoptosis-promoting gene Bax m RNA was significantly higher than that of the control group. Conclusion: metformin, as a hypoglycemic drug, can inhibit the proliferation of tongue cancer CAL27 cells in vitro. When combined, the effect of inhibiting proliferation and promoting apoptosis in CAL27 cells of tongue cancer was more significant than that of drug alone.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R739.86
本文編號：2335302
[Abstract]:Aim: to study the effects of adriamycin (ADM) and metformin (MET) on the proliferation and apoptosis of tongue cancer CAL27 cells cultured in vitro. Methods: 1. The drug concentration of MET was 5 mmol / L 10 mmol / L and 20 mmol / L = 40 mmol / L = 80 mmol / L = 160 mmol / L = 0.02 mg / L 0.04 mg / L 0.08 mg / L = 0.16 mg / L = 0.32 mg / L. After cultured tongue cancer CAL27 cells were treated for 24 h or 48 h for 72 h, the absorbance of each drug group was measured by CCK-8 method, and the inhibition rate of the cells was calculated. SPSS 17.0 software was used to calculate the concentration of IC50; that inhibited 50% cell growth in two groups of drugs alone. However, in the combined treatment group, the CAL27 cells in the IC50 MET group were treated with IC50 for 48 hours, and the absorbance value was measured by enzyme labeling instrument, and the cell inhibition rate of the combined treatment group was calculated. 2. According to the data of CCK-8 experimental group, 10 mmol / L MET semi-inhibitory concentration (0.05mg/L) of 48 h and their combination were used to detect the apoptosis of CAL27 cells by flow cytometry. 3. Q RT-PCR technique was used to detect the relative expression of Bax and Bcl-2 m RNA in CAL27 cells of tongue cancer treated with MET 10 mmol / L 0.05mg/L and MET 10mmol/L ADM 0.05mg/L for 48 h. Results: 1.CCK-8 data showed that the proliferation of tongue cancer CAL27 cells was inhibited by MET and ADM alone for 24 h or 48 h or 72 h, and in a dose-dependent and time-dependent manner. The inhibitory effect of the two drugs on the proliferation of CAL27 cells was more obvious than that of the drug alone. 2. 2. The results of flow cytometry showed that MET 10mmol/L ADM 0.05mg/L combined with drugs could promote cell apoptosis more than that of single drug group. 3. Q RT-PCR showed antiapoptotic genes in CAL27 cells of tongue cancer combined with MET and ADM. The relative expression level of Bcl-2 was more down-regulated than that of the control group. The relative expression of apoptosis-promoting gene Bax m RNA was significantly higher than that of the control group. Conclusion: metformin, as a hypoglycemic drug, can inhibit the proliferation of tongue cancer CAL27 cells in vitro. When combined, the effect of inhibiting proliferation and promoting apoptosis in CAL27 cells of tongue cancer was more significant than that of drug alone.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R739.86
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