【摘要】：目的:應(yīng)用動(dòng)物模型探討血卟啉單甲醚(hematoporphyrinmonomethyl ether,HMME)介導(dǎo)的熒光光譜法輔助診斷早期口腔癌的可行性。方法:21只金黃地鼠隨機(jī)分成3組(對(duì)照組、5周組、9周組),每組7只,各組動(dòng)物由DMBA誘導(dǎo)雙側(cè)頰囊相應(yīng)時(shí)間后,按10ml/kg經(jīng)腹腔注射20mg/L HMME溶液,用光譜診斷裝置分別記錄自體及HMME熒光,應(yīng)用Origin7.0軟件計(jì)算熒光比值K值,檢測(cè)位點(diǎn)經(jīng)病理切片HE染色定性.計(jì)算各組K值均值,SPSS10.0統(tǒng)計(jì)分析。結(jié)果:動(dòng)物實(shí)驗(yàn)結(jié)果經(jīng)方差分析,3組K值均值相比有統(tǒng)計(jì)學(xué)意義(P0.01)。結(jié)論:HMME介導(dǎo)的熒光光譜法能夠區(qū)分正常、異常增生和癌變組織,對(duì)口腔早期癌的診斷具有重要參考價(jià)值。
[Abstract]:Objective: to investigate the feasibility of hematoporphyrin monomethyl ether (hematoporphyrinmonomethyl ether,HMME)-mediated fluorescence spectrometry in the diagnosis of early oral cancer. Methods: Twenty-one golden hamsters were randomly divided into three groups (control group, 5-week group, 9-week group), 7 in each group. After inducing bilateral cheek pouch by DMBA, the rats in each group were intraperitoneally injected with 20mg/L HMME solution according to 10ml/kg. Autofluorescence and HMME fluorescence were recorded by spectral diagnostic device. The fluorescence ratio K value was calculated by Origin7.0 software. The site was determined by HE staining in pathological sections. The average K value and SPSS10.0 statistical analysis were calculated. Results: the results of animal experiments were statistically significant (P0.01). Conclusion: HMME-mediated fluorescence spectrometry can distinguish normal, abnormal hyperplasia and cancerous tissue, and has important reference value for early diagnosis of oral cancer.
【作者單位】： 哈爾濱醫(yī)科大學(xué)附屬第一醫(yī)院口腔預(yù)防保健科;哈爾濱醫(yī)科大學(xué)附屬第一醫(yī)院口腔頜面外科;
【基金】：哈醫(yī)大一院科研基金(項(xiàng)目編號(hào):2014B19)
【分類號(hào)】：R739.8

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 孟憲瑞;劉進(jìn)忠;;口腔癌的早期診斷[J];國(guó)際口腔醫(yī)學(xué)雜志;2008年03期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 黃乃艷;馬忠順;王穎;曾晶;邱海霞;唐曉英;顧瑛;;Influence of laser power density on damage of comb by photodynamic therapy—simulation and validation of mathematical models[J];Chinese Optics Letters;2014年03期

2 黃紅杰;平飛云;胡濟(jì)安;趙士芳;;Notch1和表皮生長(zhǎng)因子受體在舌鱗癌及癌前病變中的表達(dá)[J];華西口腔醫(yī)學(xué)雜志;2009年06期

3 黃玉珊;黃玉萍;李升;李春男;;口腔癌早期篩查方法的系統(tǒng)評(píng)價(jià)證據(jù)[J];井岡山大學(xué)學(xué)報(bào)(自然科學(xué)版);2011年02期

4 時(shí)偉紅;李，

本文編號(hào)：2312095