【摘要】：目的·分離牙齦卟啉單胞菌(Pg)脂多糖(LPS)及莢膜多糖(CPS),分析兩者體外誘導(dǎo)人單核細(xì)胞系THP-1細(xì)胞分泌IL-8的能力。方法·采用凝膠過濾色譜法分離已獲得的Pg ATCC33277及SJD4多糖混合物,并作用于THP-1細(xì)胞。采用ELISA法檢測THP-1細(xì)胞在不同菌株LPS和CPS的不同濃度及不同作用時間下,其上清液中IL-8的含量。結(jié)果·成功分離獲得ATCC33277及SJD4的LPS及CPS,其相對分子質(zhì)量分別為20 000~50 000及150 000~500 000;分離所得LPS及CPS均具有體外誘導(dǎo)THP-1細(xì)胞分泌IL-8的能力,且存在時間-劑量效應(yīng);在0.1μg/m L濃度下,模式菌株ATCC33277的LPS誘導(dǎo)能力均顯著高于其CPS(均P0.05),而臨床菌株SJD4在作用24 h時其CPS的誘導(dǎo)能力顯著高于LPS(P0.05)。結(jié)論·CPS作為Pg多糖提取物的組成成分,可單獨體外誘導(dǎo)THP-1細(xì)胞分泌IL-8,且存在時間-劑量效應(yīng)。
[Abstract]:Objective to isolate (Pg) lipopolysaccharide (LPS) and capsular polysaccharide (CPS),) from Porphyromonas gingivalis and analyze their ability to induce IL-8 secretion by human monocyte THP-1 cells in vitro. Methods the mixture of Pg ATCC33277 and SJD4 polysaccharides was separated by gel filtration chromatography and acted on THP-1 cells. The content of IL-8 in supernatant of THP-1 cells was determined by ELISA assay under different concentration and time of LPS and CPS. Results the relative molecular weight of LPS and CPS, of ATCC33277 and SJD4 were 20 000 ~ 50 000 and 150 000 ~ 500 000, respectively. Both LPS and CPS had the ability of inducing THP-1 cells to secrete IL-8 in vitro and had a time dose effect. At the concentration of 0.1 渭 g / mL, the LPS induction ability of the model strain ATCC33277 was significantly higher than that of its CPS (P0.05), while that of clinical strain SJD4 was significantly higher than that of LPS at 24 h (P0.05). Conclusion CPS, as a component of Pg polysaccharide extract, can induce IL-8, secretion of THP-1 cells in vitro in a time dose dependent manner.
【作者單位】： 上海交通大學(xué)醫(yī)學(xué)院附屬第九人民醫(yī)院牙周病科上海市口腔醫(yī)學(xué)重點實驗室;
【基金】：上海市科學(xué)技術(shù)委員會基礎(chǔ)研究重點項目(12JC1407502);上海市科學(xué)技術(shù)委員會醫(yī)學(xué)重點項目(13411951300) 上海高校高峰高原學(xué)科建設(shè)項目~~
【分類號】：R781.42

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