口腔扁平苔蘚患者唾液中差異蛋白的檢測及ABCG2在口腔扁平苔蘚癌變過程中的表達研究
發(fā)布時間：2018-10-25 12:03
【摘要】：第一部分口腔扁平苔蘚患者唾液中差異蛋白的檢測目的:口腔扁平苔蘚(Oral Lichen Planus,OLP)是一種較為常見且發(fā)病機制尚不明確的口腔黏膜疾病,該病頑固難治易復發(fā)且有一定的惡變傾向。本研究對病理證實為口腔扁平苔蘚的患者與健康對照組唾液中的差異蛋白進行研究,以及分析化濕行瘀清熱方治療口腔扁平苔蘚不同類型患者前后唾液中差異蛋白的表達情況。探討口腔扁平苔蘚的發(fā)病機制及化濕行瘀清熱方對口腔扁平苔蘚的治療效果及作用機制。方法:1采用酶聯(lián)免疫吸附實驗(ELISA)方法對口腔扁平苔蘚患者(20例)和健康志愿者(20例)唾液中Ig M、sAA、ZAG的含量進行測定,分析IgM、sAA、ZAG在唾液中的表達情況。2采用采用ELISA方法對口腔扁平苔蘚糜爛型(10例)和非糜爛型(10例)患者唾液中IgM、sAA、ZAG的含量進行測定,分析Ig M、sAA、ZAG在不同類型OLP患者唾液中的表達情況。3采用ELISA方法對化濕行瘀清熱方治療口腔扁平苔蘚患者(20例)前后唾液中IgM、sAA、ZAG的含量進行測定,分析化濕行瘀清熱方對OLP相關(guān)因子表達的影響及作用機制。4對化濕行瘀清熱方治療OLP不同類型患者(糜爛型與非糜爛型各50例)臨床療效進行觀測,并進行統(tǒng)計學分析。結(jié)果:1口腔扁平苔蘚患者唾液中IgM、sAA、ZAG的表達較對照組健康人唾液中IgM、sAA、ZAG表達水平高,差異有統(tǒng)計學意義(P0.05);2口腔扁平苔蘚糜爛型與非糜爛型患者唾液中IgM、sAA的表達水平無明顯差異�？谇槐馄教μ\糜爛型患者唾液中ZAG的表達較非糜爛型患者唾液中ZAG的表達高,差異有統(tǒng)計學意義(P0.05);3口腔扁平苔蘚患者經(jīng)化濕行瘀清熱方治療后唾液中Ig M、sAA、ZAG的表達水平較治療前患者唾液中IgM、sAA、ZAG表達水平降低,差異有統(tǒng)計學意義(P0.05);4化濕行瘀清熱方治療口腔扁平苔蘚不同類型患者均有良好的臨床效果,但糜爛型組與非糜爛型組無統(tǒng)計學差異。第二部分ABCG2在口腔扁平苔蘚癌變過程中的表達研究目的:研究ABCG2在口腔扁平苔蘚(Oral Lichen Planus,OLP)及口腔扁平苔蘚相關(guān)鱗狀細胞癌(Oral Lichen Planus Related Oral Squamous Cell Carcinoma,OLP-OSCC)組織中的表達情況,并行統(tǒng)計學分析。探討ABCG2是否可以作為預測OLP癌變的早期檢測指標,以期為OSCC患者的臨床診斷提供新的標志物。方法:本實驗采用免疫組化方法檢測30例OLP病變組織、8例OLP-OSCC病變組織中ABCG2蛋白表達情況,并比較ABCG2在OLP組織和OLP-OSCC組織中表達水平的差異。結(jié)果:ABCG2在OLP及OLP-OSCC組織中表現(xiàn)為高表達,細胞質(zhì)黃染,OLP組中的表達水平較OLP-OSCC組低(Z=-4.103,P=0.000)。結(jié)論:1 OLP患者唾液中IgM、s AA、ZAG可能參與OLP的發(fā)病過程,有望成為OLP的差異蛋白。2 ZAG在OLP糜爛型中較非糜爛型高表達,可能參與了OLP的發(fā)展,甚至惡化過程。3化濕行瘀清熱方治療OLP不同類型患者臨床效果顯著。4 ABCG2高表達與OLP的惡變有一定的相關(guān)性,可能積極參與了OLP惡變過程。
[Abstract]:Objective: oral lichen planus (Oral Lichen Planus,OLP) is a common oral mucosal disease with unclear pathogenesis. In this study, the differential proteins in saliva of patients with oral lichen planus proved by pathology and healthy control group were studied, and the expression of differentially expressed proteins in saliva of different types of patients with oral lichen planus before and after treatment with Huashingxingyu Qingre recipe was analyzed. To explore the pathogenesis of oral lichen planus (OLP) and the therapeutic effect and mechanism of Huishingxingyu Qingrefang (HXYQR) on oral lichen planus (OLP). Methods: 1Enzyme-linked immunosorbent assay (ELISA) was used to determine the content of Ig mapsAAZAG in saliva of 20 patients with oral lichen planus (OLP) and 20 healthy volunteers. The expression of IgM,sAA,ZAG in saliva was analyzed. (2) the content of IgM,sAA,ZAG in saliva of patients with oral lichen planus (10 cases) and non-erosive type (10 cases) was determined by ELISA method. To analyze the expression of Ig mecsAAAZAG in saliva of different types of OLP patients. (3) to measure the content of IgM,sAA,ZAG in saliva of 20 patients with oral lichen planus before and after treatment by ELISA method. The effect and mechanism of Huazhingxingyu Qingrefang on the expression of OLP related factors were analyzed. 4 the clinical efficacy of Huazhingxingyu Qingrefang in treating different types of OLP patients (50 cases of erosive type and 50 cases of non-erosive type) was observed and statistically analyzed. Results: 1the expression of IgM,sAA,ZAG in saliva of patients with oral lichen planus was significantly higher than that of healthy controls (P0.05), and the expression of IgM,sAA in saliva of patients with erosive and non-erosive oral lichen planus had no significant difference. The expression of ZAG in saliva of patients with oral lichen planus was higher than that of non-erosive patients. The difference was statistically significant (P0.05); 3 the expression level of Ig mens-AAAZAG in saliva of patients with oral lichen planus was lower than that of patients with oral lichen planus before treatment, and the expression level of IgM,sAA,ZAG in saliva of patients with oral lichen planus was lower than that of patients with oral lichen planus before treatment. The difference was statistically significant (P0.05); 4 the treatment of different types of oral lichen planus patients had good clinical effect, but there was no statistical difference between erosive group and non-erosive group. The second part of the study on the expression of ABCG2 in oral lichen planus carcinogenesis objective: to study the expression of ABCG2 in oral lichen planus (Oral Lichen Planus,OLP) and oral lichen planus associated squamous cell carcinoma (Oral Lichen Planus Related Oral Squamous Cell Carcinoma,OLP-OSCC) tissues and statistical analysis. To explore whether ABCG2 can be used as an early marker for predicting the canceration of OLP, and to provide a new marker for clinical diagnosis of OSCC patients. Methods: immunohistochemical method was used to detect the expression of ABCG2 protein in 30 cases of OLP and 8 cases of OLP-OSCC, and to compare the expression level of ABCG2 in OLP and OLP-OSCC. Results: the expression of ABCG2 in OLP and OLP-OSCC tissues was high and the cytoplasm was yellow stained. The expression level of ABCG2 in OLP group was lower than that in OLP-OSCC group (ZP0. 000). Conclusion: 1 IgM,s AA,ZAG in saliva of OLP patients may be involved in the pathogenesis of OLP and may be the differential protein of OLP. 2 the expression of ZAG in OLP erosive type is higher than that in non-erosive type, and it may be involved in the development of OLP. The clinical effect of Huazhingyu Qingrefang on different types of OLP patients was remarkable. 4 the high expression of ABCG2 was related to the malignancy of OLP, which may be actively involved in the process of OLP malignancy.
【學位授予單位】：河北醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R781.5
[Abstract]:Objective: oral lichen planus (Oral Lichen Planus,OLP) is a common oral mucosal disease with unclear pathogenesis. In this study, the differential proteins in saliva of patients with oral lichen planus proved by pathology and healthy control group were studied, and the expression of differentially expressed proteins in saliva of different types of patients with oral lichen planus before and after treatment with Huashingxingyu Qingre recipe was analyzed. To explore the pathogenesis of oral lichen planus (OLP) and the therapeutic effect and mechanism of Huishingxingyu Qingrefang (HXYQR) on oral lichen planus (OLP). Methods: 1Enzyme-linked immunosorbent assay (ELISA) was used to determine the content of Ig mapsAAZAG in saliva of 20 patients with oral lichen planus (OLP) and 20 healthy volunteers. The expression of IgM,sAA,ZAG in saliva was analyzed. (2) the content of IgM,sAA,ZAG in saliva of patients with oral lichen planus (10 cases) and non-erosive type (10 cases) was determined by ELISA method. To analyze the expression of Ig mecsAAAZAG in saliva of different types of OLP patients. (3) to measure the content of IgM,sAA,ZAG in saliva of 20 patients with oral lichen planus before and after treatment by ELISA method. The effect and mechanism of Huazhingxingyu Qingrefang on the expression of OLP related factors were analyzed. 4 the clinical efficacy of Huazhingxingyu Qingrefang in treating different types of OLP patients (50 cases of erosive type and 50 cases of non-erosive type) was observed and statistically analyzed. Results: 1the expression of IgM,sAA,ZAG in saliva of patients with oral lichen planus was significantly higher than that of healthy controls (P0.05), and the expression of IgM,sAA in saliva of patients with erosive and non-erosive oral lichen planus had no significant difference. The expression of ZAG in saliva of patients with oral lichen planus was higher than that of non-erosive patients. The difference was statistically significant (P0.05); 3 the expression level of Ig mens-AAAZAG in saliva of patients with oral lichen planus was lower than that of patients with oral lichen planus before treatment, and the expression level of IgM,sAA,ZAG in saliva of patients with oral lichen planus was lower than that of patients with oral lichen planus before treatment. The difference was statistically significant (P0.05); 4 the treatment of different types of oral lichen planus patients had good clinical effect, but there was no statistical difference between erosive group and non-erosive group. The second part of the study on the expression of ABCG2 in oral lichen planus carcinogenesis objective: to study the expression of ABCG2 in oral lichen planus (Oral Lichen Planus,OLP) and oral lichen planus associated squamous cell carcinoma (Oral Lichen Planus Related Oral Squamous Cell Carcinoma,OLP-OSCC) tissues and statistical analysis. To explore whether ABCG2 can be used as an early marker for predicting the canceration of OLP, and to provide a new marker for clinical diagnosis of OSCC patients. Methods: immunohistochemical method was used to detect the expression of ABCG2 protein in 30 cases of OLP and 8 cases of OLP-OSCC, and to compare the expression level of ABCG2 in OLP and OLP-OSCC. Results: the expression of ABCG2 in OLP and OLP-OSCC tissues was high and the cytoplasm was yellow stained. The expression level of ABCG2 in OLP group was lower than that in OLP-OSCC group (ZP0. 000). Conclusion: 1 IgM,s AA,ZAG in saliva of OLP patients may be involved in the pathogenesis of OLP and may be the differential protein of OLP. 2 the expression of ZAG in OLP erosive type is higher than that in non-erosive type, and it may be involved in the development of OLP. The clinical effect of Huazhingyu Qingrefang on different types of OLP patients was remarkable. 4 the high expression of ABCG2 was related to the malignancy of OLP, which may be actively involved in the process of OLP malignancy.
【學位授予單位】：河北醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R781.5
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