APN-1在糖尿病牙周炎小鼠牙齦組織的表達(dá)
發(fā)布時(shí)間：2018-10-22 08:11
【摘要】：研究發(fā)現(xiàn),低脂聯(lián)素血癥與T2DM發(fā)展過程中的高胰島素血癥和胰島素抵抗有關(guān)。故有學(xué)者提出,脂聯(lián)素也可作為探討患者將來發(fā)生糖尿病的一個(gè)獨(dú)立因子。在牙周炎和糖尿病的發(fā)生發(fā)展機(jī)制過程中,炎癥反應(yīng)失調(diào)和加劇是關(guān)鍵,有研究報(bào)道,對(duì)伴有糖尿病牙周炎患者進(jìn)行牙周干預(yù)治療后,患者血清脂聯(lián)素水平上升,牙周及全身狀況得到改善,有可能減少糖尿病及其并發(fā)癥的發(fā)生和發(fā)展。然而,糖尿病伴牙周炎患者的局部炎癥狀況及其參與機(jī)體胰島素抵抗的發(fā)生發(fā)展是否均與脂聯(lián)素水平變化有關(guān),這些問題都尚未得到解決。通過動(dòng)物實(shí)驗(yàn),建立伴有糖尿病的牙周炎動(dòng)物模型,研究脂聯(lián)素與血糖水平及炎癥狀態(tài)是否具有相關(guān)性,為研究糖尿病與牙周炎的相關(guān)性及日益增多的糖尿病伴有牙周炎患者的臨床治療及預(yù)防提供一些理論依據(jù)。 目的：通過檢測(cè)健康、糖尿病及糖尿病伴牙周炎昆明系小鼠牙齦組織中脂聯(lián)素基因及蛋白含量的變化,檢測(cè)三組鼠的血漿中脂聯(lián)素含量,探討脂聯(lián)素含量與血糖水平及牙周炎癥狀態(tài)是否具有相關(guān)性。 方法：45只3周齡的昆明系小鼠按不同造模方式分為三組： A組對(duì)照組(15只),標(biāo)準(zhǔn)顆粒飼料喂養(yǎng)。 B組糖尿病組(15只)。 15只健康鼠通過四氧嘧啶(Alloxan)注射法4周建立小鼠糖尿病模型(12mg/100g體重來誘導(dǎo))。 C組糖尿病+牙周炎模型(15只)。 15只健康鼠先與B組同時(shí)建糖尿病模型,糖尿病組造模成功后左、右上頜第一磨牙及第二磨牙牙頸部絲線結(jié)扎聯(lián)合高糖喂養(yǎng)6周建立小鼠糖尿病+牙周炎模型。 通過測(cè)檢AB兩組小鼠不同時(shí)間的血糖、空腹胰島素水平(Fins)及胰島素抵抗指數(shù),并與空白對(duì)照組對(duì)比來確認(rèn)小鼠糖尿病組造模成功。通過牙周組織病理組織學(xué)檢查及CBCT掃描觀察牙槽骨喪失程度判斷小鼠糖尿病+牙周炎模型造模成功。 分別取三組小鼠的牙齦組織,用RT-PCR、western blot檢測(cè)牙齦組織中脂聯(lián)素基因及蛋白含量的變化,并于鼠處死時(shí)取三組鼠的血漿,通過ELISA方法測(cè)量血漿中脂聯(lián)素含量進(jìn)行對(duì)比和統(tǒng)計(jì)學(xué)分析。 結(jié)果： 1.本實(shí)驗(yàn)發(fā)現(xiàn),B組造模后20天,小鼠血糖、空腹胰島素水平(Fins)及胰島素抵抗指數(shù)大于正常值,與空白對(duì)照組相比差異具有統(tǒng)計(jì)學(xué)意義(P0.01),小鼠糖尿病組造模成功。 2.牙齦組織病理組織學(xué)檢測(cè)顯示糖尿病伴牙周炎組小鼠牙齦炎癥表現(xiàn)最明顯、在單純糖尿病組部分小鼠可見牙齦組織輕度炎癥、健康組小鼠牙齦未見明顯炎癥；CBCT掃描結(jié)果顯示糖尿病伴牙周炎組小鼠牙槽骨喪失程度最明顯,糖尿病組小鼠次之,健康組小鼠未見明顯骨喪失。小鼠糖尿病+牙周炎組造模成功。 3.RT-PCR、western blot檢測(cè)小鼠牙齦組織中脂聯(lián)素基因及蛋白表達(dá)水平在三組間比較均有差異,存在統(tǒng)計(jì)學(xué)意義(P0.05)。在健康組表達(dá)水平最強(qiáng),在糖尿病組、糖尿病伴牙周炎組小鼠中表達(dá)水平依次減弱。 4. ELISA結(jié)果顯示APN蛋白在健康組小鼠血漿中表達(dá)水平最強(qiáng),在糖尿病組、糖尿病伴牙周炎組小鼠血漿中表達(dá)水平依次減弱;組間比較均有差異,存在統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論： 1.可通過四氧嘧啶(Alloxan)注射法有效建立小鼠糖尿病模型。可通過牙周局部結(jié)扎聯(lián)合高糖喂養(yǎng)有效建立小鼠糖尿病伴牙周炎模型。 2.糖尿病伴牙周炎組小鼠與糖尿病組小鼠、健康組小鼠相比,牙齦炎癥及骨喪失程度最明顯,脂聯(lián)素基因和蛋白含量在小鼠牙齦組織中表達(dá)顯著降低。提示糖尿病可能通過降低脂聯(lián)素水平促進(jìn)牙齦局部炎癥的發(fā)生、發(fā)展；與此同時(shí),牙周炎可能協(xié)同降低脂聯(lián)素水平,促進(jìn)糖尿病的發(fā)展。 3.糖尿病后牙齦組織及血漿中脂聯(lián)素表達(dá)降低可能是牙周炎發(fā)病及加重的重要因素之一,APN可能在糖尿病和牙周炎相互影響關(guān)系中起著一定作用,是糖尿病的保護(hù)性因子,且有一定的抗炎作用。為臨床治療和預(yù)防牙周炎提供理論依據(jù)。
[Abstract]:It has been found that hypolipoproteinemia is associated with hyperinsulinemia and insulin resistance in the progression of T2DM. Therefore, it is suggested that adiponectin can also be used as an independent factor to explore the future occurrence of diabetes in patients. In the pathogenesis of periodontitis and diabetes, inflammatory response and intensification are key, and studies have been conducted to improve serum adiponectin levels, periodontal and systemic conditions after periodontal intervention in patients with diabetic periodontitis. It is possible to reduce the occurrence and development of diabetes and its complications. However, the local inflammatory conditions of diabetic patients with periodontitis and their involvement in the development of body insulin resistance are related to changes in adiponectin levels, which have not been resolved. animal model of periodontitis with diabetes is established by animal experiment to study whether adiponectin is relevant to blood sugar level and inflammation state, To study the correlation between diabetes mellitus and periodontitis and the increasing number of diabetes patients with periodontitis, some theoretical bases are provided. Objective: To investigate the changes of adiponectin gene and protein content in gingival tissues of Kunming mice with periodontitis and periodontitis. Methods: Forty-five-week-old Kunming mice were treated with different models. The formula is divided into three groups: A group control group (15 only), Standard pellet feed. Group B diabetes mellitus group (15 rats). 15 healthy rats established the model of diabetes mellitus (1) in 4 weeks by Alloxin injection method. 2mg/ 100g body weight). Group C diabetes + periodontitis model (15 rats). 15 healthy rats were first established with group B while the model of diabetes mellitus was constructed. The model of diabetic + periodontitis in mice was established by high sugar feeding for 6 weeks. The blood glucose, fasting insulin level (Fins) and insulin resistance index of two groups of mice were measured. and compared with the blank control group to confirm the success of the model of the diabetic group of the mice. The pathological histology examination of the periodontal tissue and the CBCT scanning observation teeth To determine the degree of loss of bone loss, the model of diabetic + periodontitis was established successfully. The gingival tissues of three groups of mice were taken respectively, and the changes of adiponectin gene and protein content in gingival tissues were detected by RT-PCR and Western blot. LIS The content of adiponectin in plasma was compared and analyzed by A method. Results: 1. The results showed that the blood glucose, fasting insulin level (Fins) and insulin resistance index of mice were more than normal in 20 days after group B modeling and blank Compared with the control group, the difference was statistically significant (P0.01), and the model of diabetic group was successful. There was no significant inflammation in the gum tissue in the healthy group of mice in the pure diabetic group, and the CBCT scan showed diabetes mellitus with periodontitis. The alveolar bone loss of the group was the most obvious, the sugar The mice showed no obvious bone loss in the group of urine disease group. The model of diabetic + periodontitis group was successful. RT-PCR and western blot were used to detect the gingival tissues of mice. There was a significant difference in the levels of adiponectin gene and protein expression among the three groups (P0.05). in diabetic group, that expression level of APN protein in healthy group of mice was the strongest, and the expression level of APN protein in healthy group was the strongest in diabetic group and diabetic group. periodontitis The levels of expression in plasma of group mice were decreased in turn; there was a difference between the groups, and there was statistical significance (P 0. 05). Conclusion: 1. Available through Alloxa n) The mice model of diabetes mellitus can be effectively established by injection method. The model of diabetic companion periodontitis can be effectively established by the combination of periodontal local ligation and high-sugar feeding. Compared with the group of mice and healthy mice, the degree of gingival inflammation and bone loss was most obvious, and the expression of adiponectin gene and protein in the gingival tissues of mice was significantly decreased. Decrease the level of adiponectin to promote local inflammation of the gum, develop; At the same time, periodontitis may work synergistically to reduce the level of adiponectin and promote the development of diabetes. One of the factors is that APN may be in diabetes and periodontal
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R587.2;R781.42
本文編號(hào)：2286607
[Abstract]:It has been found that hypolipoproteinemia is associated with hyperinsulinemia and insulin resistance in the progression of T2DM. Therefore, it is suggested that adiponectin can also be used as an independent factor to explore the future occurrence of diabetes in patients. In the pathogenesis of periodontitis and diabetes, inflammatory response and intensification are key, and studies have been conducted to improve serum adiponectin levels, periodontal and systemic conditions after periodontal intervention in patients with diabetic periodontitis. It is possible to reduce the occurrence and development of diabetes and its complications. However, the local inflammatory conditions of diabetic patients with periodontitis and their involvement in the development of body insulin resistance are related to changes in adiponectin levels, which have not been resolved. animal model of periodontitis with diabetes is established by animal experiment to study whether adiponectin is relevant to blood sugar level and inflammation state, To study the correlation between diabetes mellitus and periodontitis and the increasing number of diabetes patients with periodontitis, some theoretical bases are provided. Objective: To investigate the changes of adiponectin gene and protein content in gingival tissues of Kunming mice with periodontitis and periodontitis. Methods: Forty-five-week-old Kunming mice were treated with different models. The formula is divided into three groups: A group control group (15 only), Standard pellet feed. Group B diabetes mellitus group (15 rats). 15 healthy rats established the model of diabetes mellitus (1) in 4 weeks by Alloxin injection method. 2mg/ 100g body weight). Group C diabetes + periodontitis model (15 rats). 15 healthy rats were first established with group B while the model of diabetes mellitus was constructed. The model of diabetic + periodontitis in mice was established by high sugar feeding for 6 weeks. The blood glucose, fasting insulin level (Fins) and insulin resistance index of two groups of mice were measured. and compared with the blank control group to confirm the success of the model of the diabetic group of the mice. The pathological histology examination of the periodontal tissue and the CBCT scanning observation teeth To determine the degree of loss of bone loss, the model of diabetic + periodontitis was established successfully. The gingival tissues of three groups of mice were taken respectively, and the changes of adiponectin gene and protein content in gingival tissues were detected by RT-PCR and Western blot. LIS The content of adiponectin in plasma was compared and analyzed by A method. Results: 1. The results showed that the blood glucose, fasting insulin level (Fins) and insulin resistance index of mice were more than normal in 20 days after group B modeling and blank Compared with the control group, the difference was statistically significant (P0.01), and the model of diabetic group was successful. There was no significant inflammation in the gum tissue in the healthy group of mice in the pure diabetic group, and the CBCT scan showed diabetes mellitus with periodontitis. The alveolar bone loss of the group was the most obvious, the sugar The mice showed no obvious bone loss in the group of urine disease group. The model of diabetic + periodontitis group was successful. RT-PCR and western blot were used to detect the gingival tissues of mice. There was a significant difference in the levels of adiponectin gene and protein expression among the three groups (P0.05). in diabetic group, that expression level of APN protein in healthy group of mice was the strongest, and the expression level of APN protein in healthy group was the strongest in diabetic group and diabetic group. periodontitis The levels of expression in plasma of group mice were decreased in turn; there was a difference between the groups, and there was statistical significance (P 0. 05). Conclusion: 1. Available through Alloxa n) The mice model of diabetes mellitus can be effectively established by injection method. The model of diabetic companion periodontitis can be effectively established by the combination of periodontal local ligation and high-sugar feeding. Compared with the group of mice and healthy mice, the degree of gingival inflammation and bone loss was most obvious, and the expression of adiponectin gene and protein in the gingival tissues of mice was significantly decreased. Decrease the level of adiponectin to promote local inflammation of the gum, develop; At the same time, periodontitis may work synergistically to reduce the level of adiponectin and promote the development of diabetes. One of the factors is that APN may be in diabetes and periodontal
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R587.2;R781.42
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相關(guān)期刊論文 前2條
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2 釋棟;孟煥新;徐莉;張立;陳智濱;馮向輝;;牙周炎患者的血脂、血糖水平分析[J];中華口腔醫(yī)學(xué)雜志;2006年07期
，本文編號(hào)：2286607
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