【摘要】：細(xì)菌感染是牙髓炎的主要病因,常由于各種原因的牙體硬組織破壞,細(xì)菌入侵牙髓而誘發(fā)感染。G-菌是牙髓炎的主要致病菌,胞壁中的LPS是其主要毒力因子,同宿主其他部位一樣,牙髓炎癥是宿主對病原體的免疫反應(yīng),牙髓組織破壞和修復(fù)之間的關(guān)系決定了牙髓組織的活力及其炎癥程度。在宿主免疫反應(yīng)中,免疫細(xì)胞及非免疫細(xì)胞能夠表達(dá)模式識別受體(PPR)來識別并結(jié)合病原體,進(jìn)而誘導(dǎo)炎癥反應(yīng)。DC-SIGN是模式識別受體C型凝集素中的成員,它既能激活初始免疫應(yīng)答,又能抑制免疫反應(yīng),具有正負(fù)免疫調(diào)節(jié)作用,是近年來研究的熱點(diǎn)。DC-SIGN主要分布在樹突狀細(xì)胞和巨噬細(xì)胞中,兼具細(xì)胞黏附受體和模式識別受體功能,在特異性及非特異性免疫反應(yīng)中都發(fā)揮作用。目前關(guān)于DC-SIGN在人炎性牙髓組織中的作用未見報(bào)道,其在牙髓疾病中的發(fā)生、發(fā)展的作用機(jī)理也尚不清楚。本實(shí)驗(yàn)應(yīng)用免疫組織化學(xué)檢測法初步探討DC-SIGN在人牙髓炎癥過程中的表達(dá)及作用。實(shí)驗(yàn)?zāi)康?應(yīng)用免疫組織化學(xué)的方法,觀察DC-SIGN在正常及急、慢性人牙髓組織中的表達(dá)和定位情況,并對其結(jié)果進(jìn)行統(tǒng)計(jì)學(xué)分析,初步探討DC-SIGN在人牙髓炎中的作用機(jī)制。實(shí)驗(yàn)方法:收集于吉林大學(xué)口腔醫(yī)院就診患者的新鮮牙髓組織,經(jīng)固定、包埋、切片、HE染色,應(yīng)用光學(xué)顯微鏡觀察牙髓組織病理學(xué)變化,并結(jié)合患者的臨床表現(xiàn),作為牙髓組織分組依據(jù),將組織標(biāo)本分為三組。其中,正常牙髓組鏡下表現(xiàn)為成纖維細(xì)胞、未分化間充質(zhì)細(xì)胞、毛細(xì)血管及膠原纖維;急性炎癥牙髓組鏡下表現(xiàn)為血管擴(kuò)張充血,中性粒細(xì)胞廣泛浸潤,局部組織液化壞死;慢性炎癥牙髓組鏡下表現(xiàn)為血管擴(kuò)張充血,組織水腫,淋巴細(xì)胞、漿細(xì)胞、巨噬細(xì)胞、中性粒細(xì)胞等慢性炎癥細(xì)胞浸潤。免疫組織化學(xué)方法觀察DC-SIGN在三組標(biāo)本中的定位和表達(dá)情況。使用顯微照相系統(tǒng)和Image-pro-plus圖像分析軟件對各組DC-SIGN陽性部位進(jìn)行平均光密度測定,進(jìn)行統(tǒng)計(jì)學(xué)處理,評價(jià)三組牙髓組織中DC-SIGN的定位和表達(dá)情況。結(jié)果:HE染色觀察到急慢性炎癥牙髓組中血管擴(kuò)張充血,炎性細(xì)胞廣泛浸潤。免疫組織化學(xué)法觀察到,三組牙髓組織中均有DC-SIGN表達(dá),但不同組別其平均光密度值不同,DC-SIGN在正常牙髓組織中表達(dá)少,在炎癥性牙髓組織中表達(dá)增多。結(jié)論:DC-SIGN在人炎癥性牙髓組織中有表達(dá),并參與牙髓炎的發(fā)生、發(fā)展過程。
[Abstract]:Bacterial infection is the main cause of pulpitis, which is often induced by the destruction of hard tissue of dental body and the invasion of dental pulp by bacteria. G- bacteria is the main pathogen of pulpitis, and LPS in the cell wall is the main virulence factor. Just like other parts of the host, dental pulp inflammation is the host's immune response to pathogens. The relationship between the destruction and repair of dental pulp tissue determines the activity of dental pulp tissue and the degree of inflammation. In the host immune response, immune cells and non-immune cells can express pattern recognition receptor (PPR) to recognize and bind pathogens, and then induce inflammatory response. DC-SIGN is a member of pattern recognition receptor C lectin. It not only activates the initial immune response, but also inhibits the immune response. It has both positive and negative immunomodulatory effects, and is a hot topic in recent years. DC-SIGN mainly distributes in dendritic cells and macrophages, and has the functions of cell adhesion receptor and pattern recognition receptor. Play a role in both specific and nonspecific immune responses. At present, the role of DC-SIGN in human inflammatory dental pulp tissue has not been reported, and the mechanism of its occurrence and development in dental pulp disease is still unclear. The expression and role of DC-SIGN in human dental pulp inflammation were studied by immunohistochemical method. Objective: to observe the expression and localization of DC-SIGN in normal, acute and chronic human dental pulp by immunohistochemical method, and to analyze the results statistically, and to explore the mechanism of DC-SIGN in human pulpitis. Methods: the fresh dental pulp tissue was collected from the patients in Jilin University Stomatology Hospital. The pathological changes of dental pulp were observed by optical microscope after fixation, embedding, sectioning, HE staining, and combined with the clinical manifestations of the patients. As the basis of pulp tissue grouping, tissue specimens were divided into three groups. Among them, fibroblasts, undifferentiated mesenchymal cells, capillaries and collagen fibers were observed under microscope in the normal dental pulp group, and vasodontic hyperemia, extensive infiltration of neutrophils and local tissue liquefaction and necrosis were observed in the acute inflammatory dental pulp group. The chronic inflammatory pulp group showed vasodontic hyperemia, tissue edema, infiltration of lymphocytes, plasma cells, macrophages, neutrophils and other chronic inflammatory cells. Immunohistochemical method was used to observe the localization and expression of DC-SIGN in the three groups. The mean optical density of the positive parts of DC-SIGN in each group was measured by microphotography system and Image-pro-plus image analysis software. The location and expression of DC-SIGN in three groups of dental pulp tissues were evaluated by statistical analysis. Results: vascular dilation and hyperemia and extensive infiltration of inflammatory cells were observed in acute and chronic inflammatory pulp group by HE staining. The expression of DC-SIGN was observed by immunohistochemical method in the three groups, but the average optical density was different in different groups. The expression of DC-SIGN was less in the normal dental pulp tissue and increased in the inflammatory pulp tissue. Conclusion: DC-SIGN is expressed in human inflammatory pulp tissue and is involved in the occurrence and development of pulpitis.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R781

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