【摘要】：目的展示乳牙列、混合牙列、和恒牙列的健康樣本與高齲樣本唾液菌群的細(xì)菌圖譜,并對(duì)菌群的物種豐度、多樣性與群落結(jié)構(gòu)進(jìn)行比較分析,認(rèn)識(shí)齲病發(fā)生與發(fā)展的菌群失調(diào)過程,從而指導(dǎo)建立不同時(shí)期齲病風(fēng)險(xiǎn)預(yù)測模型,同時(shí)為齲病的個(gè)體化預(yù)防、治療和風(fēng)險(xiǎn)評(píng)估提供理論基礎(chǔ)。方法選擇150名4-16歲的廣州中小學(xué)生,并收集齲病和健康學(xué)生的唾液樣品,提取基因組DNA,擴(kuò)增16S r RNA高變區(qū)片段,進(jìn)行454高通量測序,利用統(tǒng)計(jì)學(xué)方法分析不同組別之間菌群的差異。結(jié)果本研究發(fā)現(xiàn):(1)通過對(duì)口腔唾液菌種多樣性分析發(fā)現(xiàn):健康和高齲組的物種豐度指數(shù)及物種多樣性指數(shù)均存在顯著差異(p0.001),高齲組的菌種多樣性高于健康組;三種牙列間菌群物種豐度和多樣性均存在顯著差異,其中乳牙列組菌群的多樣性指數(shù)均與高于混合牙列組與恒牙列組,而混合牙列組與恒牙列組間無顯著差異(p0.05);(2)通過對(duì)口腔菌種群落結(jié)構(gòu)的比較發(fā)現(xiàn):健康人之間的群落結(jié)構(gòu)更為相似保守,而高齲人群的群落結(jié)構(gòu)則更為分散(p0.01);對(duì)三種牙列期菌群結(jié)構(gòu)進(jìn)行比較,發(fā)現(xiàn)乳牙列期兒童人群口腔菌群結(jié)構(gòu)更為分散,而混合牙列和恒牙列結(jié)構(gòu)更為相似保守,且恒牙列的群落結(jié)構(gòu)保守性顯著高于混合牙列;(3)通過對(duì)不同分類水平的口腔唾液細(xì)菌的圖譜進(jìn)行比較:在不同牙列期未發(fā)現(xiàn)齲特異性細(xì)菌種類,但均可檢測到在高齲人群和健康人群中,發(fā)現(xiàn)相對(duì)豐度存在顯著差別分布的細(xì)菌種類,如放線菌(Actinobacteria),變形菌(Proteobacteria),TM7,擬桿菌(Bacteroidetes),梭桿菌(Fusobacteria),螺旋體(Spirochaetes)等。結(jié)論:1.口腔疾病狀態(tài)和宿主年齡均在一定程度上影響唾液菌群的組成與結(jié)構(gòu)。2.未發(fā)現(xiàn)與齲病相關(guān)的特異性細(xì)菌的存在,但可以發(fā)現(xiàn)相對(duì)豐度存在顯著差異的菌種,推測齲病的發(fā)生可能是源于群落失調(diào)所致。
[Abstract]:Objective to show the bacterial profiles of salivary flora in healthy and high caries samples of deciduous dentition, mixed dentition and permanent dentition, and to compare the species abundance, diversity and community structure of the flora. In order to guide the establishment of dental caries risk prediction models in different periods, it can provide a theoretical basis for individualized prevention, treatment and risk assessment of caries. Methods 150 primary and middle school students aged 4 to 16 years old in Guangzhou were selected, and saliva samples of dental caries and healthy students were collected. The 16s r RNA hypervariable region was amplified by genomic DNA, and 454 high throughput sequencing was carried out. The differences between different groups of bacteria were analyzed by statistical method. Results the results showed that: (1) there were significant differences in species abundance index and species diversity index between healthy and high caries groups (p0.001), and the species diversity in high caries group was higher than that in healthy group. There were significant differences in species abundance and diversity among the three dentition groups, and the diversity index of the primary dentition group was higher than that of the mixed dentition group and the permanent dentition group, and the diversity index of the deciduous dentition group was higher than that of the mixed dentition group and the permanent dentition group. However, there was no significant difference between the mixed dentition group and the permanent dentition group (p0. 05); (2). By comparing the community structure of oral bacteria species, it was found that the community structure of healthy people was more similar and conservative. The community structure of the high caries population was more dispersed (p0.01), and compared with the three dentition groups, the oral microflora structure of the primary dentition children was more dispersed, while the mixed dentition and permanent dentition were similar and conservative. The conserved community structure of permanent dentition was significantly higher than that of mixed dentition. (3) by comparing the profiles of oral saliva bacteria at different classification levels, no caries-specific bacteria were found in different dentition. However, there were significant differences in the distribution of relative abundance between the high caries population and the healthy population, such as actinomycetes (Actinobacteria), mutant (Proteobacteria) TM7, Clostridium (Bacteroidetes), (Fusobacteria), spirochetes (Spirochaetes) and so on. Conclusion 1. Oral disease state and host age affect the composition and structure of saliva flora to some extent. No specific bacteria related to caries were found, but there were significant differences in relative abundance, which suggested that the occurrence of caries might be caused by community imbalance.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R788.1

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